SDS-PAGE Sample Loading Buffer

Item Number

S750307

• Product Name: SDS-PAGE Sample Loading Buffer
• Specifications & Purity: 2X

Product Description

SDS-PAGE Sample Loading Buffer, 2X is a modified 2x concentrated protein loading buffer with bromophenol blue dye. It can be routinely used for the preparation and loading of protein samples onto a gel for SDS-PAGE analysis.

Precautions：

1.This product contains a small amount of DTT with a slightly irritating odor, but does not contain the highly toxic mercaptoethanol.2. This product must be completely thawed and mixed well prior to use.3. This product is for R&D only. Not for drug, household, or other uses.4.For your safety and health, please wear a lab coat and disposable gloves during the operation.

Instructions for Use：

1. Dissolve SDS-PAGE Sample Loading Buffer (2X) at room temperature or in a water bath not exceeding 37ºC. Place this product at room temperature immediately after dissolution to avoid prolonged storage in water bath.2. Mix protein samples and Sample Loading Buffer (2X) at a ratio of 1:1. For example, mix 1µl of protein sample with 1µl of Sample Loading Buffer (2X).3. Heat at 100ºC for 3-5 min to fully denature proteins.Note: If the starting material is large and the genomic DNA content is high, the mixture may be very sticky after boiling. In this case, boil for another 5-10 minutes or after addition of more 1X sample loading buffer. The thorough boiling will release proteins bound to genomic DNA and break genomic DNA so that the mixture will be not too sticky for sample loading.4. After cooling to room temperature, centrifuge briefly at room temperature, then load the supernatant onto a SDS-PAGE gel. 5. Stop electrophoresis when the bromophenol blue reaches the bottom of the gel.