Uelandy serum free cell crYOpreservation solution is a ready to use product without serum and animal derived protein. Therefore, it not only reduces the pollution risk of all kinds of bacteria, viruses, mycoplasma, etc., and ensures the safety of frozen cells, but also reduces the impact of exogenous proteins on the normal growth and differentiation of cells. In addition, without the need for programmed cooling, cells can be stored at -80 ℃ for a long time, which is suitable for the crYOpreservation of most mammalian cells.
Matters needing attention：
1. before use, ensure that the cell cryopreservation solution is completely melted and gently mixed. The thawed cell cryopreservation solution is stored at 4 º C and is effective within three months. 2. this product contains phenol red indicator. Long time exposure will lead to pH change and precipitation. Therefore, please try to shorten the exposure time during use. After use, close the cover tightly and store it. If necessary, it can be properly subpackaged for use. If a small amount of precipitate appears after using for a period of time, it has no effect on cell cryopreservation after testing. To remove the precipitate, centrifuge the supernatant, 0.22 μ M pore size filter filtration and other methods. 3. please ensure that the cells are in good condition before freezing, and the proportion of viable cells should be greater than 90%. 4. this product contains DMSO, and some cells are sensitive to DMSO. It is recommended to conduct pre experiment. 5. for some precious cells, it is recommended to use the conventional freezing solution containing FBS for comparison test at the same time, and then conduct formal freezing after confirming the performance. 6. after subpackaging, the frozen cells should be moved into the -80 º C ultra-low temperature refrigerator as soon as possible to reduce the storage time outside. 7. for your safety and health, please wear experimental clothes and disposable gloves.
