SID 3712249 (MiR-544 Inhibitor 1) is a miR-544 biogenesis inhibitor. SID 3712249 binds directly to the precursor miRNA. SID 3712249 blocks production of the mature microRNA and decreases miR-544, HIF-1α, and ATM transcripts. SID 3712249 can be used in the research of cancers, such as breast cancer
In Vitro
SID 3712249 (compound 1, 20 nM, 48 h) disrupts miR-544-mediated inhibition of BMI1 based on significant increases in the RFP/BFP ratio. SID 3712249 (20 nM, 48 h) inhibits precursor but not mature miR-544 binding to BMI1 or mTOR3’-UTR. SID 3712249 (20 nM, 48 h) results in accumulation of pre-miR-544 and a decrease in miR-544 levels in MCF10A cells. SID 3712249 (20 nM, 5 days) induces apoptosis in MDA-MB-231 and MCF- 7 cells and has no effect on cell survival in normoxic conditions. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Apoptosis AnalysisCell Line: MDA-MB-231, MCF-7 cells Concentration: 20 nM Incubation Time: 5 days Result: Induced apoptosis and has no effect on cell survival in normoxic conditions (flow cytometric analysis of Annexin V and PI stainning).
In Vivo
SID 3712249 (compound 1, 100 μL of 40 μM, intraperitoneal injection) inhibited tumor growth in MDA-MB-231-GFP-luc tumor model . SID 3712249 (20 nM, pre-treated GFP-labeled MDA-MB-231 cells, intraperitoneal injection) inhibited tumor growth in MDA-MB-231-GFP-luc tumor model . MCE has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model: MDA-MB-231-GFP-luc tumor model Dosage: 100 μL of 40 μM Administration: Intraperitoneal injection Result: Inhibited tumor growth (evidenced by live animal bioluminescent imaging) with no overt side effects. Decreased levels of miR-544, ATM, and HIF-1α and increased levels of mTOR (resected tumor samples).
