This cell line is used in studies of apoptosis and neuroprotection as an in vitro model of human neuronal cells.
Specifications & Purity
Mycoplasma free
Images
SK-N-SH Whole Cell Lysate (cl185705) - Western Blot All lanes: beta Actin Mouse mAb (Ab155822) at 1/2000 dilution Lane 1: A549 Whole Cell Lysate (cl155906) Lane 2: HeLa Whole Cell Lysate (cl155894) Lane 3: LNCaP Whole Cell Lysate (cl155902) Lane 4: SK-N-SH Whole Cell Lysate (cl185705) Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution Predicted band size: 42 kDa Observed band size: 41 kDa Exposure time: 21.1 s
SK-N-SH Whole Cell Lysate (cl185705) - Western Blot All lanes: Recombinant PCNA Antibody (Ab120681) at 1/2000 dilution Lane 1: A549 Whole Cell Lysate (cl155906) Lane 2: HeLa Whole Cell Lysate (cl155894) Lane 3: LNCaP Whole Cell Lysate (cl155902) Lane 4: SK-N-SH Whole Cell Lysate (cl185705) Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution Predicted band size: 29 kDa Observed band size: 31 kDa Exposure time: 113.4 s
SK-N-SH Whole Cell Lysate (cl185705) - Western Blot All lanes: COX IV Mouse mAb (Ab097414) at 1/2000 dilution Lane 1: A549 Whole Cell Lysate (cl155906) Lane 2: HeLa Whole Cell Lysate (cl155894) Lane 3: LNCaP Whole Cell Lysate (cl155902) Lane 4: SK-N-SH Whole Cell Lysate (cl185705) Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution Predicted band size: 20 kDa Observed band size: 17 kDa Exposure time: 22.9 s
Product Specifications
Form
Lyophilized
Reconstitution
Recommended reconstitute at 1-2 mg/mL in ddH₂O. Use at an assay dependent concentration. Ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.Lyophilized contents may appear as either a translucent film or a white pow
Storage Temp
Store at -80°C
Shipped In
Ice chest + Ice pads
Stability And Storage
Shipped on Dry Ice. Lyophilizate is recommended stored at -80℃. After reconstitution, recommended aliquot and avoid repeated freeze-thaw, store at -20℃ for short-term storage or -80℃ for long-term storage.
