Small RNA Ladder

Item Number

S750554

• Specifications & Purity: 10-50nt, 9 bands
• Stability And Storage: Store at -80℃, valid for at least 1 year.
• Storage Temp: Store at -80°C
• Shipped In: Ice chest + Ice pads

Product Description

Aladdin's Small RNA Ladder (10-50nt, 9 bands) consists of nine single-stranded RNA oligonucleotides ranging from 10 to 50nt in length. Both the 5' and the 3' ends of each oligonucleotide are hydroxyl groups, enabling it to be used as a small RNA size marker during the conventional single-stranded small RNA electrophoresis analysis and detection.The size of these nine RNA oligonucleotides in the ladder is evenly distributed to facilitate small RNA size approximation.The ladder is an ideal choice for using 10%-20% polyacrylamide gel containing urea (7M, 7.5M, or 8M) or in 3% agarose gel to analyze small RNA molecules ranging from 10-50nt. A good staining result can be obtained by Na-red (an EB upgraded product, 2000X) (Figure 1). The 30nt band is brighter, making the ladder bands easier to be differentiated.The ladder is prepared in DEPC water containing 50% deionized formamide. This product is sufficient for 50 loadings when 2μl of ladder is loaded per well.Figure 1

Precautions：

RNAase is ubiquitous in surroundings. Please take extreme care to avoid RNase contamination during the use of Small RNA Ladder. Wear a mask always during the operation. After melting, keep it on ice and make aliquots to avoid freeze-thaw cycles. Close the lid immediately after use to minimize the exposure of Small RNA Ladder to air.This product is recommended for small RNA size approximation, not for accurate size determination, because small RNA molecules may contain some compositions that affect their mobility during electrophoresis.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.

Instructions for Use：

1. Prepare urea-PAGE gel with appropriate concentrations of polyacrylamide and urea, or 3% agarose gel for electrophoresis. We recommend the Urea-PAGE Gel Preparation Kit (, R0218S) for the preparation of urea-PAGE gels.2. For denatured urea-PAGE, mix 2μl ladder and 2μl of 2X RNA Loading Buffer (, R0215) well, boil at 95℃ for 1 minute, and immediately put it on ice. Load 4μl of mixture onto a urea-PAGE gel for electrophoresis.3. For agarose gel electrophoresis, mix 2μl of ladder and 2μl of 2X RNA Loading Buffer (, R0215) well, and load the 4μl mixture onto agarose gel directly for electrophoresis.4. After electrophoresis, transfer the gel into a clean container with an appropriate size, wash the gel with DEPC water for 1-2 minutes, stain the gel with 30ml of nucleic acid staining solution for 15 minutes at room temperature on a shaker at 25rpm, wash with DEPC water 2-3 times (2-5 minutes each time), and then observe the staining result by a gel imaging system. We recommend using the NA-Red (EB upgraded product, 2000X) for RNA gel staining and using DEPC water for the dilution.Related Products:产品编号产品名称包装R0206-100μlSmall RNA Ladder (10-50nt, 9 bands)100μlR0215-1ml2X RNA Loading Buffer1mlR0218SUrea-PAGE Preparation Kit for RNA