pAb; Rabbit anti Human SMN/Gemin 1 Antibody; WB, ICC, IF; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab128362-10μl | 10μl | In stock | $59.90 | |
| Ab128362-50μl | 50μl | In stock | $169.90 | |
| Ab128362-100μl | 100μl | In stock | $269.90 | |
| Ab128362-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $1,899.90 |
pAb; Rabbit anti Human SMN/Gemin 1 Antibody; WB, ICC, IF; Unconjugated
| Product Name | SMN/Gemin 1 Antibody - Primary antibody, specific to SMN1, Rabbit IgG |
|---|---|
| Synonyms | Component of gems 1 | Gemin-1 | BCD541 antibody | Component of gems 1 antibody | Gemin 1 antibody | Gemin-1 antibody | OTTHUMP00000125198 antibody | OTTHUMP00000223567 antibody | OTTHUMP00000223568 antibody | OTTHUMP00000224066 antibody | OTTHUMP000002269 |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Azide Free, High performance, Lot by Lot |
| Host species | Rabbit |
| Specificity | SMN1 |
| Immunogen | Recombinant SMN expressed in E.coli (AA 2-294) |
| Positive Control | WB: HeLa, HEK293, Hep G2, A431 and Raji cell lysates. ICC/IF: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, High performance, Validated |
| Product Description | Rabbit anti Human SMN/Gemin 1 Antibody, Polyclonal, could be used for WB, ICC, IF and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified + Protein A purified |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
SMN/Gemin 1 Antibody (Ab128362) - Western Blot
All lanes: SMN/Gemin 1 Antibody (Ab128362) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 32 kDa
Observed band size: 36 kDa
Exposure time: 17.1 s
SMN/Gemin 1 Antibody (Ab128362) - IF/ICC
FITC staining on IF
Samples: Human HeLa Cells.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB |
0.01-2 µg/mL
|
| ICC/IF |
5-20 µg/mL
|
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0404451 | Certificate of Analysis | Apr 11, 2024 | Ab128362 |
| ZJ24F0404452 | Certificate of Analysis | Apr 11, 2024 | Ab128362 |
| ZJ24F0404453 | Certificate of Analysis | Apr 11, 2024 | Ab128362 |
| 1. Ratni H, Ebeling M, Baird J, Bendels S, Bylund J, Chen KS, Denk N, Feng Z, Green L, Guerard M et al.. (2018) Discovery of Risdiplam, a Selective Survival of Motor Neuron-2 ( SMN2) Gene Splicing Modifier for the Treatment of Spinal Muscular Atrophy (SMA).. J Med Chem, 61 (15): (6501-6517). [PMID:30044619] |
| 2. Jingbo Xiao,Juan J Marugan,Wei Zheng,Steve Titus,Noel Southall,Jonathan J Cherry,Matthew Evans,Elliot J Androphy,Christopher P Austin. (2011-08-09) Discovery, synthesis, and biological evaluation of novel SMN protein modulators.. Journal of medicinal chemistry, 54 ((18)): (6215-6233). [PMID:21819082] |
| 3. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330] |
| 4. Yanzhong Yang,Andrea Hadjikyriacou,Zheng Xia,Sitaram Gayatri,Daehoon Kim,Cecilia Zurita-Lopez,Ryan Kelly,Ailan Guo,Wei Li,Steven G Clarke,Mark T Bedford. (2015-03-05) PRMT9 is a type II methyltransferase that methylates the splicing factor SAP145.. Nature communications, 6 (6428-6428). [PMID:25737013] |
| 5. Bach M, Lehmann A, Brünnert D, Vanselow JT, Hartung A, Bargou RC, Holzgrabe U, Schlosser A, Chatterjee M.. (2017) Ugi Reaction-Derived α-Acyl Aminocarboxamides Bind to Phosphatidylinositol 3-Kinase-Related Kinases, Inhibit HSF1-Dependent Heat Shock Response, and Induce Apoptosis in Multiple Myeloma Cells.. J Med Chem, 60 (10): (4147-4160). [PMID:28453931] |
| 6. Rietz A, Li H, Quist KM, Cherry JJ, Lorson CL, Burnett BG, Kern NL, Calder AN, Fritsche M, Lusic H, Boaler PJ, Choi S, Xing X, Glicksman MA, Cuny GD, Androphy EJ, Hodgetts KJ.. (2017) Discovery of a Small Molecule Probe That Post-Translationally Stabilizes the Survival Motor Neuron Protein for the Treatment of Spinal Muscular Atrophy.. J Med Chem, 60 (11): (4594-4610). [PMID:28481536] |
| 7. Choi S, Calder AN, Miller EH, Anderson KP, Fiejtek DK, Rietz A, Li H, Cherry JJ, Quist KM, Xing X, Glicksman MA, Cuny GD, Lorson CL, Androphy EA, Hodgetts KJ.. (2017) Optimization of a series of heterocycles as survival motor neuron gene transcription enhancers.. Bioorg Med Chem Lett, 27 (23): (5144-5148). [PMID:29103974] |
| 8. van der Steege, G G and 9 more authors.. (1995) A provisional transcript map of the spinal muscular atrophy (SMA) critical region.. European journal of human genetics : EJHG, [PMID:7552146] |
| 9. Gennarelli, M M and 7 more authors.. (1995) Survival motor neuron gene transcript analysis in muscles from spinal muscular atrophy patients.. Biochemical and biophysical research communications, (4): [PMID:7639755] |
| 10. Lefebvre, S S and 9 more authors.. (1995) Identification and characterization of a spinal muscular atrophy-determining gene.. Cell, (13): [PMID:7813012] |
| 11. Liu, Q Q and Dreyfuss, G G.. (1996) A novel nuclear structure containing the survival of motor neurons protein.. The EMBO journal, (15): [PMID:8670859] |
| 12. Bürglen, L L and 7 more authors.. (1996) Structure and organization of the human survival motor neurone (SMN) gene.. Genomics, (15): [PMID:8838816] |
| 13. Talbot, K K and 6 more authors.. (1997) Missense mutation clustering in the survival motor neuron gene: a role for a conserved tyrosine and glycine rich region of the protein in RNA metabolism?. Human molecular genetics, [PMID:9147655] |
| 14. Hahnen, E E and 5 more authors.. (1997) Missense mutations in exon 6 of the survival motor neuron gene in patients with spinal muscular atrophy (SMA).. Human molecular genetics, [PMID:9158159] |
| 15. Coovert, D D DD and 9 more authors.. (1997) The survival motor neuron protein in spinal muscular atrophy.. Human molecular genetics, [PMID:9259265] |
| 16. Liu, Q Q, Fischer, U U, Wang, F F and Dreyfuss, G G.. (1997) The spinal muscular atrophy disease gene product, SMN, and its associated protein SIP1 are in a complex with spliceosomal snRNP proteins.. Cell, (19): [PMID:9323129] |
| 17. Chen, Q Q and 10 more authors.. (1998) Sequence of a 131-kb region of 5q13.1 containing the spinal muscular atrophy candidate genes SMN and NAIP.. Genomics, (15): [PMID:9503025] |
| 18. Parsons, D W DW and 5 more authors.. (1998) Intragenic telSMN mutations: frequency, distribution, evidence of a founder effect, and modification of the spinal muscular atrophy phenotype by cenSMN copy number.. American journal of human genetics, [PMID:9837824] |
| 19. Pellizzoni, L L, Kataoka, N N, Charroux, B B and Dreyfuss, G G.. (1998) A novel function for SMN, the spinal muscular atrophy disease gene product, in pre-mRNA splicing.. Cell, (25): [PMID:9845364] |
| 20. Wang, C H CH, Papendick, B D BD, Bruinsma, P P and Day, J K JK.. (1998) Identification of a novel missense mutation of the SMN(T) gene in two siblings with spinal muscular atrophy.. Neurogenetics, [PMID:10732802] |
| 21. Rochette, C F CF and 7 more authors.. (1997) Molecular diagnosis of non-deletion SMA patients using quantitative PCR of SMN exon 7.. Neurogenetics, [PMID:10732817] |
| 22. Selenko, P P and 5 more authors.. (2001) SMN tudor domain structure and its interaction with the Sm proteins.. Nature structural biology, [PMID:11135666] |
| 23. Gangwani, L L, Mikrut, M M, Theroux, S S, Sharma, M M and Davis, R J RJ.. (2001) Spinal muscular atrophy disrupts the interaction of ZPR1 with the SMN protein.. Nature cell biology, [PMID:11283611] |
| 24. Kurihara, N N, Menaa, C C, Maeda, H H, Haile, D J DJ and Reddy, S V SV.. (2001) Osteoclast-stimulating factor interacts with the spinal muscular atrophy gene product to stimulate osteoclast formation.. The Journal of biological chemistry, (2): [PMID:11551898] |
| 25. Mourelatos, Z Z, Abel, L L, Yong, J J, Kataoka, N N and Dreyfuss, G G.. (2001) SMN interacts with a novel family of hnRNP and spliceosomal proteins.. The EMBO journal, (1): [PMID:11574476] |
| 26. Hebert, M D MD, Szymczyk, P W PW, Shpargel, K B KB and Matera, A G AG.. (2001) Coilin forms the bridge between Cajal bodies and SMN, the spinal muscular atrophy protein.. Genes & development, (15): [PMID:11641277] |
| 27. Baccon, Jennifer J, Pellizzoni, Livio L, Rappsilber, Juri J, Mann, Matthias M and Dreyfuss, Gideon G.. (2002) Identification and characterization of Gemin7, a novel component of the survival of motor neuron complex.. The Journal of biological chemistry, (30): [PMID:12065586] |
| 28. Paushkin, Sergey S, Gubitz, Amélie K AK, Massenet, Séverine S and Dreyfuss, Gideon G.. (2002) The SMN complex, an assemblyosome of ribonucleoproteins.. Current opinion in cell biology, [PMID:12067652] |
| 29. Narayanan, Usha U, Ospina, Jason K JK, Frey, Mark R MR, Hebert, Michael D MD and Matera, A Gregory AG.. (2002) SMN, the spinal muscular atrophy protein, forms a pre-import snRNP complex with snurportin1 and importin beta.. Human molecular genetics, (15): [PMID:12095920] |
| 30. Pillai, Ramesh S RS and 6 more authors.. (2003) Unique Sm core structure of U7 snRNPs: assembly by a specialized SMN complex and the role of a new component, Lsm11, in histone RNA processing.. Genes & development, (15): [PMID:12975319] |
| 31. Hua, Yimin Y and Zhou, Jianhua J.. (2004) Rpp20 interacts with SMN and is re-distributed into SMN granules in response to stress.. Biochemical and biophysical research communications, (30): [PMID:14715275] |
| 32. Cuscó, I I, Barceló, M Jesus MJ, del Río, E E, Baiget, M M and Tizzano, E F EF.. (2004) Detection of novel mutations in the SMN Tudor domain in type I SMA patients.. Neurology, (13): [PMID:15249625] |
| 33. Schmutz, Jeremy J and 75 more authors.. (2004) The DNA sequence and comparative analysis of human chromosome 5.. Nature, (16): [PMID:15372022] |
| 34. Sun, Y Y and 5 more authors.. (2005) Molecular and functional analysis of intragenic SMN1 mutations in patients with spinal muscular atrophy.. Human mutation, [PMID:15580564] |
| 35. Azzouz, Teldja N TN and 7 more authors.. (2005) Toward an assembly line for U7 snRNPs: interactions of U7-specific Lsm proteins with PRMT5 and SMN complexes.. The Journal of biological chemistry, (14): [PMID:16087681] |
| 36. van Koningsbruggen, Silvana S and 6 more authors.. (2007) FRG1P-mediated aggregation of proteins involved in pre-mRNA processing.. Chromosoma, [PMID:17103222] |
| 37. Otter, Simon S and 5 more authors.. (2007) A comprehensive interaction map of the human survival of motor neuron (SMN) complex.. The Journal of biological chemistry, (23): [PMID:17178713] |
| 38. Piazzon, Nathalie N and 5 more authors.. (2008) In vitro and in cellulo evidences for association of the survival of motor neuron complex with the fragile X mental retardation protein.. The Journal of biological chemistry, (29): [PMID:18093976] |
| 39. Daub, Henrik H and 9 more authors.. (2008) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle.. Molecular cell, (8): [PMID:18691976] |
| 40. Chari, Ashwin A and 8 more authors.. (2008) An assembly chaperone collaborates with the SMN complex to generate spliceosomal SnRNPs.. Cell, (31): [PMID:18984161] |
| 41. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332] |
| 42. Mahmoudi, Salah S and 7 more authors.. (2010) WRAP53 is essential for Cajal body formation and for targeting the survival of motor neuron complex to Cajal bodies.. PLoS biology, (2): [PMID:21072240] |
| 43. Hubers, Lisa L and 5 more authors.. (2011) HuD interacts with survival motor neuron protein and can rescue spinal muscular atrophy-like neuronal defects.. Human molecular genetics, (1): [PMID:21088113] |
| 44. Fallini, Claudia C and 6 more authors.. (2011) The survival of motor neuron (SMN) protein interacts with the mRNA-binding protein HuD and regulates localization of poly(A) mRNA in primary motor neuron axons.. The Journal of neuroscience : the official journal of the Society for Neuroscience, (9): [PMID:21389246] |
| 45. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692] |
| 46. Wu, Chia-Yen and 9 more authors.. (2011) Identification of the phosphorylation sites in the survival motor neuron protein by protein kinase A.. Biochimica et biophysica acta, [PMID:21609790] |
| 47. Skourti-Stathaki, Konstantina K, Proudfoot, Nicholas J NJ and Gromak, Natalia N.. (2011) Human senataxin resolves RNA/DNA hybrids formed at transcriptional pause sites to promote Xrn2-dependent termination.. Molecular cell, (24): [PMID:21700224] |
| 48. Zhang, Rundong R and 6 more authors.. (2011) Structure of a key intermediate of the SMN complex reveals Gemin2's crucial function in snRNP assembly.. Cell, (5): [PMID:21816274] |
| 49. Tripsianes, Konstantinos K and 7 more authors.. (2011) Structural basis for dimethylarginine recognition by the Tudor domains of human SMN and SPF30 proteins.. Nature structural & molecular biology, (20): [PMID:22101937] |
| 50. Bienvenut, Willy V WV and 6 more authors.. (2012) Comparative large scale characterization of plant versus mammal proteins reveals similar and idiosyncratic N-α-acetylation features.. Molecular & cellular proteomics : MCP, [PMID:22223895] |
| 51. Sarachan, Kathryn L KL and 8 more authors.. (2012) Solution structure of the core SMN-Gemin2 complex.. The Biochemical journal, (1): [PMID:22607171] |
| 52. Van Damme, Petra P and 15 more authors.. (2012) N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.. Proceedings of the National Academy of Sciences of the United States of America, (31): [PMID:22814378] |
| 53. Martin, Renee R, Gupta, Kushol K, Ninan, Nisha S NS, Perry, Kay K and Van Duyne, Gregory D GD.. (2012) The survival motor neuron protein forms soluble glycine zipper oligomers.. Structure (London, England : 1993), (7): [PMID:23022347] |
| 54. Lotti, Francesco and 10 more authors.. (2012) An SMN-dependent U12 splicing event essential for motor circuit function.. Cell, (12): [PMID:23063131] |
| 55. Gupta, Kushol K and 5 more authors.. (2015) Oligomeric Properties of Survival Motor Neuron·Gemin2 Complexes.. The Journal of biological chemistry, (14): [PMID:26092730] |
| 56. Zhao, Dorothy Yanling DY and 14 more authors.. (2016) SMN and symmetric arginine dimethylation of RNA polymerase II C-terminal domain control termination.. Nature, (7): [PMID:26700805] |
| 57. Hao le, Thi T and 6 more authors.. (2017) HuD and the Survival Motor Neuron Protein Interact in Motoneurons and Are Essential for Motoneuron Development, Function, and mRNA Regulation.. The Journal of neuroscience : the official journal of the Society for Neuroscience, (29): [PMID:29061699] |
| 58. Yi, Hongfei and 6 more authors.. (2020) Negative cooperativity between Gemin2 and RNA provides insights into RNA selection and the SMN complex's release in snRNP assembly.. Nucleic acids research, (24): [PMID:31799625] |
