Purity>95% (SDS-PAGE&SEC); Endotoxin Level<1.0EU/mg; Human IgG1; CHO; ELISA, FACS, Functional assay, Animal Model; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab170552-100μg | 100μg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $49.90 | |
| Ab170552-1mg | 1mg | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $237.90 | |
| Ab170552-5mg | 5mg | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $567.90 | |
| Ab170552-10mg | 10mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $927.90 |
Purity>95% (SDS-PAGE&SEC); Endotoxin Level<1.0EU/mg; Human IgG1; CHO; ELISA, FACS, Functional assay, Animal Model; Unconjugated
| Product Name | Sotigalimab (anti-CD40) - Primary antibody, for ELISA,Functional Assay,Kinetics (BLI),Kinetics (SPR),Flow Cyt various applications, specific to CD40, >95%, high purity, Human IgG1 |
|---|---|
| Synonyms | Sotigalimab; sotiga; AI326936 antibody; B cell associated molecule CD40 antibody; B cell surface antigen CD40 antibody; B cell-associated molecule antibody; B-cell surface antigen CD40 antibody; Bp50 antibody; CD 40 antibody; CD40 antibody; CD40 antigen ( |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Low Endotoxin, Azide Free, Recombinant, ≥95%(SDS-PAGE&SEC), Lot by Lot |
| Specificity | CD40 |
| Application | ELISA,Functional Assay,Kinetics (BLI),Kinetics (SPR),Flow Cyt |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, Low Endotoxin, Recombinant, Validated |
| Action Type | AGONIST |
| Mechanism of action | AGONIST of Tumor necrosis factor receptor superfamily member 5 agonist |
| Product Description | Sotigalimab (anti-CD40), a CD40 agonistic monoclonal antibody, can be used for the research of metastatic pancreatic adenocarcinoma. Purity： >95% (SDS-PAGE&SEC) Endotoxin Level < 1.0EU/mg |
| Antibody Type | Primary antibody |
|---|---|
| Clonality | Recombinant |
| Isotype | Human IgG1 |
| Light Chain Type | kappa |
| SDS-PAGE | 144.54 kDa |
| Purification Method | Protein A purified |
| Purity | >95% |
| Source | CHO supernatant |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at -80°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at -80℃ for 24 months. Upon delivery aliquot. Avoid freeze/thaw cycle. |
| CAS | 2305607-45-6 |
Sotigalimab (anti-CD40) (Ab170552) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD40 with Sotigalimab (anti-CD40) (Ab170552) conjugated with biotin (right panel) compared with Human IgG (Ab170213) conjugated with biotin - Isotype Control (left panel) and detected with SA-PE (rp156250).
Sotigalimab (anti-CD40) (Ab170552) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD40 (red) with Sotigalimab (anti-CD40) (Ab170552) conjugated with biotin and detected with SA-PE (rp156250). Blue - Isotype control, human IgG (Ab170213) conjugated with biotin. Black - Unlabelled control, cells without incubation with primary antibody.
Sotigalimab (anti-CD40) (Ab170552) - Flow Cytometry
Flow Cytometry analysis of Raji cells labelling CD40 (red) with Sotigalimab (anti-CD40) (Ab170552). Goat Anti-Human IgG (PE) (Ab175838) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, human IgG (Ab170213). Black - Unlabelled control, cells without incubation with primary antibody.
Sotigalimab (anti-CD40) (Ab170552) - SEC
The purity of Sotigalimab (anti-CD40) (Ab170552) is more than 95% verified by HPLC.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F1001492 | Certificate of Analysis | Nov 02, 2023 | Ab170552 |
| ZJ23F1001491 | Certificate of Analysis | Nov 02, 2023 | Ab170552 |
| 1. Chen J, Song Y, Bojadzic D, Tamayo-Garcia A, Landin AM, Blomberg BB, Buchwald P.. (2017) Small-Molecule Inhibitors of the CD40-CD40L Costimulatory Protein-Protein Interaction.. J Med Chem, 60 (21): (8906-8922). [PMID:29024591] |
| 2. Braesch-Andersen, S S and 5 more authors.. (1989) Biochemical characteristics and partial amino acid sequence of the receptor-like human B cell and carcinoma antigen CDw40.. Journal of immunology (Baltimore, Md. : 1950), (15): [PMID:2463309] |
| 3. Stamenkovic, I I, Clark, E A EA and Seed, B B.. (1989) A B-lymphocyte activation molecule related to the nerve growth factor receptor and induced by cytokines in carcinomas.. The EMBO journal, [PMID:2475341] |
| 4. Sato, T T, Irie, S S and Reed, J C JC.. (1995) A novel member of the TRAF family of putative signal transducing proteins binds to the cytosolic domain of CD40.. FEBS letters, (23): [PMID:7530216] |
| 5. Cheng, G G and 5 more authors.. (1995) Involvement of CRAF1, a relative of TRAF, in CD40 signaling.. Science (New York, N.Y.), (10): [PMID:7533327] |
| 6. Bajorath, J J and Aruffo, A A.. (1997) Construction and analysis of a detailed three-dimensional model of the ligand binding domain of the human B cell receptor CD40.. Proteins, [PMID:9037712] |
| 7. Kashiwada, M M and 8 more authors.. (1998) Tumor necrosis factor receptor-associated factor 6 (TRAF6) stimulates extracellular signal-regulated kinase (ERK) activity in CD40 signaling along a ras-independent pathway.. The Journal of experimental medicine, (19): [PMID:9432981] |
| 8. Mizushima, S S and 8 more authors.. (1998) Cloning and characterization of a cDNA encoding the human homolog of tumor necrosis factor receptor-associated factor 5 (TRAF5).. Gene, (30): [PMID:9511754] |
| 9. Singh, J J and 7 more authors.. (1998) The role of polar interactions in the molecular recognition of CD40L with its receptor CD40.. Protein science : a publication of the Protein Society, [PMID:9605317] |
| 10. Pullen, S S SS and 5 more authors.. (1998) CD40-tumor necrosis factor receptor-associated factor (TRAF) interactions: regulation of CD40 signaling through multiple TRAF binding sites and TRAF hetero-oligomerization.. Biochemistry, (25): [PMID:9718306] |
| 11. Ni, C Z CZ and 6 more authors.. (2000) Molecular basis for CD40 signaling mediated by TRAF3.. Proceedings of the National Academy of Sciences of the United States of America, (12): [PMID:10984535] |
| 12. Tone, M M, Tone, Y Y, Fairchild, P J PJ, Wykes, M M and Waldmann, H H.. (2001) Regulation of CD40 function by its isoforms generated through alternative splicing.. Proceedings of the National Academy of Sciences of the United States of America, (13): [PMID:11172023] |
| 13. Ferrari, S S and 15 more authors.. (2001) Mutations of CD40 gene cause an autosomal recessive form of immunodeficiency with hyper IgM.. Proceedings of the National Academy of Sciences of the United States of America, (23): [PMID:11675497] |
| 14. Khandekar, S S SS and 9 more authors.. (2001) Determination of carbohydrate structures N-linked to soluble CD154 and characterization of the interactions of CD40 with CD154 expressed in Pichia pastoris and Chinese hamster ovary cells.. Protein expression and purification, [PMID:11676606] |
| 15. Li, Chenglong C and 9 more authors.. (2002) Downstream regulator TANK binds to the CD40 recognition site on TRAF3.. Structure (London, England : 1993), [PMID:12005438] |
| 16. Tai, Yu-Tzu YT and 13 more authors.. (2004) Mechanisms by which SGN-40, a humanized anti-CD40 antibody, induces cytotoxicity in human multiple myeloma cells: clinical implications.. Cancer research, (15): [PMID:15087402] |
| 17. Ouadani, Hanen H and 14 more authors.. (2016) Novel and recurrent AID mutations underlie prevalent autosomal recessive form of HIGM in consanguineous patients.. Immunogenetics, [PMID:26545377] |
| 18. Takada, Yoko K; Yu, Jessica; Shimoda, Michiko and Takada, Yoshikazu.. (2019) Integrin Binding to the Trimeric Interface of CD40L Plays a Critical Role in CD40/CD40L Signaling.. Journal of immunology (Baltimore, Md. : 1950), (1): [PMID:31331973] |
