Streptavidin and Conjugates include streptavidin in a purified form, conjugated to enzymes or fluorophores or activated for crosslinking to sulfhydryl or carbonyl groups. These purified and conjugated forms of recombinant streptavidin protein enable detection of biotinylated antibodies and other probes in a variety of standard assay methods, including Western blotting, ELISA, immunohistochemistry and fluorescence imaging. Purified streptavidin and the streptavidin-HRP conjugates (peroxidase-conjugated streptavidin) are supplied lyophilized in phosphate-based buffers for immediate reconstitution with water. Alkaline Phosphatase conjugates are supplied as a liquid in a Tris-based buffer. Applications for enzyme-labeled (HRP, AP) streptavidin: Enzyme immunoassays (EIA), including enzyme-linked immunsorbent assays (ELISA) with biotinylated antibodies Cell and tissue staining (for light microscopy) Western blotting (WB) and other membrane-based biotin detection Applications for fluorescent (FITC, TRITC or Texas Red* Fluor-labeled) streptavidin: Immunofluorescent assays (IFA) Tissue staining (for fluorescence microscopy) Blot immunostaining Cell staining (incl. fluorescent activated cell sorting)
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