pAb; Rabbit anti Human Sumo 1 Antibody; WB, IHC; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab129741-10μl | 10μl | In stock | $66.90 | |
| Ab129741-50μl | 50μl | In stock | $189.90 | |
| Ab129741-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $289.90 | |
| Ab129741-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,699.90 |
pAb; Rabbit anti Human Sumo 1 Antibody; WB, IHC; Unconjugated
| Product Name | Sumo 1 Antibody - Primary antibody, specific to Sumo 1, Rabbit IgG |
|---|---|
| Synonyms | DAP1 antibody; GAP modifying protein 1 antibody; GAP-modifying protein 1 antibody; GMP 1 antibody; GMP1 antibody; OFC10 antibody; PIC 1 antibody; PIC1 antibody; SENP2 antibody; Sentrin 1 antibody; Sentrin antibody; Small ubiquitin related modifier 1 antib |
| Specifications & Purity | ExactAb™, Validated, 0.6 mg/mL |
| Host species | Rabbit |
| Specificity | Sumo 1 |
| Immunogen | A synthetic peptide derived from human Sumo 1 (AA 84-96) |
| Positive Control | WB: HeLa, MCF7, A549, NIH/3T3 and C6 cell lysates. IHC: Human breast carcinoma tissue. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Validated |
| Product Description | Rabbit anti Human Sumo 1 Antibody, Polyclonal, could be used for WB, IHC and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 Protein Function: Ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved for instance in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. May also regulate a network of genes involved in palate development. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 0.6 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Sumo 1 Antibody (Ab129741) - Western Blot
All lanes: Sumo 1 Antibody (Ab129741) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 12 kDa
Observed band size: 18, 82 kDa
Exposure time: 56.1 s
Sumo 1 Antibody (Ab129741) - IHC
Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma using Sumo 1 Antibody (Ab129741). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
| IHC | 1/50 - 1/100 |
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0202814 | Certificate of Analysis | Feb 21, 2024 | Ab129741 |
| ZJ24F0202813 | Certificate of Analysis | Feb 21, 2024 | Ab129741 |
| 1. Dai XQ, Kolic J, Marchi P, Sipione S, Macdonald PE. (2009) SUMOylation regulates Kv2.1 and modulates pancreatic beta-cell excitability.. J Cell Sci, 122 (Pt 6): (775-9). [PMID:19223394] |
| 2. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330] |
| 3. Yan P, Ritt DA, Zlotkowski K, Bokesch HR, Reinhold WC, Schneekloth JS, Morrison DK, Gustafson KR.. (2018) Macrophilones from the Marine Hydroid Macrorhynchia philippina Can Inhibit ERK Cascade Signaling.. J Nat Prod, 81 (7): (1666-1672). [PMID:29979591] |
| 4. Boddy, M N MN, Howe, K K, Etkin, L D LD, Solomon, E E and Freemont, P S PS.. (1996) PIC 1, a novel ubiquitin-like protein which interacts with the PML component of a multiprotein complex that is disrupted in acute promyelocytic leukaemia.. Oncogene, (5): [PMID:8806687] |
| 5. Shen, Z Z, Pardington-Purtymun, P E PE, Comeaux, J C JC, Moyzis, R K RK and Chen, D J DJ.. (1996) UBL1, a human ubiquitin-like protein associating with human RAD51/RAD52 proteins.. Genomics, (1): [PMID:8812453] |
| 6. Okura, T T and 7 more authors.. (1996) Protection against Fas/APO-1- and tumor necrosis factor-mediated cell death by a novel protein, sentrin.. Journal of immunology (Baltimore, Md. : 1950), (15): [PMID:8906799] |
| 7. Matunis, M J MJ, Coutavas, E E and Blobel, G G.. (1996) A novel ubiquitin-like modification modulates the partitioning of the Ran-GTPase-activating protein RanGAP1 between the cytosol and the nuclear pore complex.. The Journal of cell biology, [PMID:8978815] |
| 8. Mahajan, R R, Delphin, C C, Guan, T T, Gerace, L L and Melchior, F F.. (1997) A small ubiquitin-related polypeptide involved in targeting RanGAP1 to nuclear pore complex protein RanBP2.. Cell, (10): [PMID:9019411] |
| 9. Lapenta, V V and 5 more authors.. (1997) SMT3A, a human homologue of the S. cerevisiae SMT3 gene, maps to chromosome 21qter and defines a novel gene family.. Genomics, (1): [PMID:9119407] |
| 10. Kamitani, T T, Nguyen, H P HP and Yeh, E T ET.. (1997) Preferential modification of nuclear proteins by a novel ubiquitin-like molecule.. The Journal of biological chemistry, (30): [PMID:9162015] |
| 11. Bayer, P P and 6 more authors.. (1998) Structure determination of the small ubiquitin-related modifier SUMO-1.. Journal of molecular biology, (10): [PMID:9654451] |
| 12. Everett, R D RD, Lomonte, P P, Sternsdorf, T T, van Driel, R R and Orr, A A.. (1999) Cell cycle regulation of PML modification and ND10 composition.. Journal of cell science, [PMID:10574707] |
| 13. Minty, A A, Dumont, X X, Kaghad, M M and Caput, D D.. (2000) Covalent modification of p73alpha by SUMO-1. Two-hybrid screening with p73 identifies novel SUMO-1-interacting proteins and a SUMO-1 interaction motif.. The Journal of biological chemistry, (17): [PMID:10961991] |
| 14. Su, Hong-Lin HL and Li, Steven S-L SS.. (2002) Molecular features of human ubiquitin-like SUMO genes and their encoded proteins.. Gene, (21): [PMID:12383504] |
| 15. Engelhardt, Othmar G OG and 5 more authors.. (2003) The homeodomain-interacting kinase PKM (HIPK-2) modifies ND10 through both its kinase domain and a SUMO-1 interaction motif and alters the posttranslational modification of PML.. Experimental cell research, (1): [PMID:12565818] |
| 16. Tatham, Michael H MH and 8 more authors.. (2003) Role of an N-terminal site of Ubc9 in SUMO-1, -2, and -3 binding and conjugation.. Biochemistry, (26): [PMID:12924945] |
| 17. Reverter, David D and Lima, Christopher D CD.. (2004) A basis for SUMO protease specificity provided by analysis of human Senp2 and a Senp2-SUMO complex.. Structure (London, England : 1993), [PMID:15296745] |
| 18. Xu, Zheng Z and Au, Shannon W N SW.. (2005) Mapping residues of SUMO precursors essential in differential maturation by SUMO-specific protease, SENP1.. The Biochemical journal, (1): [PMID:15487983] |
| 19. Tatham, Michael H MH and 5 more authors.. (2005) Unique binding interactions among Ubc9, SUMO and RanBP2 reveal a mechanism for SUMO paralog selection.. Nature structural & molecular biology, [PMID:15608651] |
| 20. Lois, Luisa Maria LM and Lima, Christopher D CD.. (2005) Structures of the SUMO E1 provide mechanistic insights into SUMO activation and E2 recruitment to E1.. The EMBO journal, (9): [PMID:15660128] |
| 21. Pichler, Andrea A and 8 more authors.. (2005) SUMO modification of the ubiquitin-conjugating enzyme E2-25K.. Nature structural & molecular biology, [PMID:15723079] |
| 22. Hillier, Ladeana W LW and 121 more authors.. (2005) Generation and annotation of the DNA sequences of human chromosomes 2 and 4.. Nature, (7): [PMID:15815621] |
| 23. Reverter, David D and Lima, Christopher D CD.. (2005) Insights into E3 ligase activity revealed by a SUMO-RanGAP1-Ubc9-Nup358 complex.. Nature, (2): [PMID:15931224] |
| 24. Baba, Daichi D and 9 more authors.. (2005) Crystal structure of thymine DNA glycosylase conjugated to SUMO-1.. Nature, (16): [PMID:15959518] |
| 25. Song, Jing J, Zhang, Ziming Z, Hu, Weidong W and Chen, Yuan Y.. (2005) Small ubiquitin-like modifier (SUMO) recognition of a SUMO binding motif: a reversal of the bound orientation.. The Journal of biological chemistry, (2): [PMID:16204249] |
| 26. Xu, Zheng Z and 5 more authors.. (2006) Crystal structure of the SENP1 mutant C603S-SUMO complex reveals the hydrolytic mechanism of SUMO-specific protease.. The Biochemical journal, (15): [PMID:16712526] |
| 27. Um, Ji Won JW and Chung, Kwang Chul KC.. (2006) Functional modulation of parkin through physical interaction with SUMO-1.. Journal of neuroscience research, (15): [PMID:16955485] |
| 28. Alkuraya, Fowzan S FS and 5 more authors.. (2006) SUMO1 haploinsufficiency leads to cleft lip and palate.. Science (New York, N.Y.), (22): [PMID:16990542] |
| 29. Shen, Linnan L and 5 more authors.. (2006) SUMO protease SENP1 induces isomerization of the scissile peptide bond.. Nature structural & molecular biology, [PMID:17099698] |
| 30. Carbia-Nagashima, Alberto A and 7 more authors.. (2007) RSUME, a small RWD-containing protein, enhances SUMO conjugation and stabilizes HIF-1alpha during hypoxia.. Cell, (19): [PMID:17956732] |
| 31. Tatham, Michael H MH and 7 more authors.. (2008) RNF4 is a poly-SUMO-specific E3 ubiquitin ligase required for arsenic-induced PML degradation.. Nature cell biology, [PMID:18408734] |
| 32. Meulmeester, Erik E, Kunze, Marion M, Hsiao, He Hsuan HH, Urlaub, Henning H and Melchior, Frauke F.. (2008) Mechanism and consequences for paralog-specific sumoylation of ubiquitin-specific protease 25.. Molecular cell, (6): [PMID:18538659] |
| 33. Daub, Henrik H and 9 more authors.. (2008) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle.. Molecular cell, (8): [PMID:18691976] |
| 34. Matic, Ivan I, Macek, Boris B, Hilger, Maximiliane M, Walther, Tobias C TC and Mann, Matthias M.. (2008) Phosphorylation of SUMO-1 occurs in vivo and is conserved through evolution.. Journal of proteome research, [PMID:18707152] |
| 35. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692] |
| 36. Hong, Yongde Y and 9 more authors.. (2011) SUMOylation of DEC1 protein regulates its transcriptional activity and enhances its stability.. PloS one, [PMID:21829689] |
| 37. Cong, Lin L, Pakala, Suresh B SB, Ohshiro, Kazufumi K, Li, Da-Qiang DQ and Kumar, Rakesh R.. (2011) SUMOylation and SUMO-interacting motif (SIM) of metastasis tumor antigen 1 (MTA1) synergistically regulate its transcriptional repressor function.. The Journal of biological chemistry, (23): [PMID:21965678] |
| 38. Li, Renfeng R and 6 more authors.. (2012) SUMO binding by the Epstein-Barr virus protein kinase BGLF4 is crucial for BGLF4 function.. Journal of virology, [PMID:22398289] |
| 39. Rabellino, Andrea A and 10 more authors.. (2012) The SUMO E3-ligase PIAS1 regulates the tumor suppressor PML and its oncogenic counterpart PML-RARA.. Cancer research, (1): [PMID:22406621] |
| 40. Sun, Huaiyu H and Hunter, Tony T.. (2012) Poly-small ubiquitin-like modifier (PolySUMO)-binding proteins identified through a string search.. The Journal of biological chemistry, (7): [PMID:23086935] |
| 41. Sun, Xiaodong X, Li, Jie J, Dong, Frederick N FN and Dong, Jin-Tang JT.. (2014) Characterization of nuclear localization and SUMOylation of the ATBF1 transcription factor in epithelial cells.. PloS one, [PMID:24651376] |
| 42. Hendriks, Ivo A; Treffers, Louise W; Verlaan-de Vries, Matty; Olsen, Jesper V and Vertegaal, Alfred C O.. (2015) SUMO-2 Orchestrates Chromatin Modifiers in Response to DNA Damage.. Cell reports, (17): [PMID:25772364] |
| 43. Yamashita, Daisuke D, Moriuchi, Takanobu T, Osumi, Takashi T and Hirose, Fumiko F.. (2016) Transcription Factor hDREF Is a Novel SUMO E3 Ligase of Mi2α.. The Journal of biological chemistry, (27): [PMID:27068747] |
