Super page gelsteinredtm nucleic acid dye, 10000 × In water
Product Description
Super Page GelstainRed nucleic acid dye is a non-toxic, non-mutagenic red nucleic acid dye that specifically stains Acrylamide gel DNA. The 254 nm ultraviolet transmission instrument equipped with EB filter can be used for imaging. Super Page GelstainRed nucleic acid dyes that bind to DNA can be effectively removed using commercial DNA gel extraction kits, or using phenol / chloroform extraction, ethanol precipitation, etc. The sensitivity of dsDNA staining with Super Page GelstainRed nucleic acid dye was 6-8 times higher than that of RNA.
Instruction： 1. Dyeing DNA in Polyamide Gel ( 1 ) Acrylamide gel electrophoresis experiment of DNA was carried out according to the routine procedure. ( 2 ) Dilute Super Page GelstainRed nucleic acid dye with deionized water, 10,000 × in water mother liquor, configure 1 × gel staining solution, such as adding 5 μL of dye to 50 mL of deionized water. ( 3 ) The PAGE gel was carefully placed in a container containing Super Page GelstainRedTM staining solution and incubated at room temperature for 30 min. For gels containing 3.5 ~ 10 % acrylamide, the dyeing time is usually between 30 min and 1 h, and extends with the increase of acrylamide content. The dyeing solution can be reused 1-2 times. ( 4 ) A 254 nm ultraviolet transmission spectrometer equipped with an EB filter was used for imaging. 2.staining DNA in agarose gel The Super Page GelstainRed nucleic acid dye can also stain the DNA of agarose gel in addition to the DNA staining of Acrylamide gel. The use of Super Page GelstainRed nucleic acid dyes should be post-dyed to obtain better results, and pre-dyeing or direct mixing with DNA samples should not be used.
Matters needing attention：
1. please centrifuge the product to the bottom of the tube immediately before use, and then conduct subsequent experiments.
2. for your safety and health, please wear experimental clothes and disposable gloves.
Scope of application：
Nucleic acid sample staining in nucleic acid gel electrophoresis
