SuperStain - 10,000× in Water, high purity

Item Number

S665827

• Specifications & Purity: 10,000× in Water
• Storage Temp: Protected from light,Room temperature
• Shipped In: Normal

Product Description

Product content

Component	S665827-500μl
SuperStain (10,000× in Water)	500 μl

Product Introduction

SuperStain is a biological macromolecule that cannot penetrate cell membranes to enter cells. Compared with the strong mutagenicity of EB, it is a safe and non-toxic nucleic acid dye.SuperStain has the same spectral characteristics with EB, no need to change the filter and observation device, it can be detected by normal UV excitation, combining the advantages of non-toxicity, high sensitivity and high stability, and it can be stained by gel staining or bubble staining, which makes it very convenient and flexible to use.

Self-contained reagents

agarose

TAE/TBE Electrophoresis Buffer

Pre-experiment Preparation and Important Notes

When dyeing with the soak dyeing method, the amount of dye is high, and a single use of the dye solution can be reused about 3 times.3 x Dye solution can be prepared in large quantities and stored at room temperature, protected from light.

If you consistently see dispersed or poorly separated bands, it is recommended that you use a bubble stain to confirm that the problem is not dye related. If the problem persists after staining, the problem is not dye-related, so try: choosing the right agarose concentration; choosing a longer gel; prolonging the gel time to ensure that the edges are clear; and decreasing the amount of DNA sampled (the recommended amount is 50-200ng/lane).

Operation steps

1. Gel dyeing method

Add SuperStain (10,000×in Water) to the agarose solution to a final concentration of 1× (5 μl of dye if the agarose solution is 50 ml) and mix thoroughly. After the gel has solidified, electrophoresis and image acquisition are performed in the usual way. Note: Due to its good thermal stability, SuperStain can be added directly to hot agarose solution without waiting for the solution to cool. Shake, oscillate or turn to ensure the dye is well mixed. Optionally, SuperStain can be added to agarose powder and electrophoresis buffer and then heated in a microwave or other commonly used means to prepare an agarose gel.

2. Dyeing method

1) Configure a dye-free agarose gel and perform electrophoresis according to conventional methods.

2) Dilute SuperStain (10,000×in Water) about 3,300 times into 0.1 M NaCl with H2O to make a 3× staining solution, e.g., add 15 μl SuperStain (10,000×in Water) and 5 ml of 1 M NaCl to 45 ml of H2O.

3) Carefully place the gel into a suitable container and slowly add sufficient amount of 3× staining solution to submerge the gel. Oscillate the staining at room temperature for about 30 min. The optimal staining time varies slightly depending on the thickness of the gel as well as the concentration of agarose. Image acquisition was performed according to conventional methods.

The graph on the left shows the electrophoresis of Super DNA Marker in an agarose gel containing SuperStain dye, with sample volumes on the main strip from left to right (150ng, 75ng).