mAb (C3); Mouse anti Human Survivin Antibody; WB, IHC; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab129838-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $66.90 | |
| Ab129838-20μl | 20μl | In stock | $89.90 | |
| Ab129838-50μl | 50μl | In stock | $189.90 | |
| Ab129838-100μl | 100μl | In stock | $299.90 | |
| Ab129838-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,299.90 |
mAb (C3); Mouse anti Human Survivin Antibody; WB, IHC; Unconjugated
| Product Name | Survivin Mouse mAb - Primary antibody, specific to Survivin, Mouse IgG1 |
|---|---|
| Synonyms | API4 antibody; Apoptosis inhibitor 4 antibody; Apoptosis inhibitor survivin antibody; Apoptosis inhibitor4 antibody; Baculoviral IAP repeat containing 5 antibody; Baculoviral IAP repeat containing protein 5 antibody; Baculoviral IAP repeat-containing prot |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Azide Free, High performance, Lot by Lot |
| Host species | Mouse |
| Specificity | Survivin |
| Immunogen | Recombinant Survivin expressed in E.coli (AA 1-142) |
| Positive Control | WB: HEK293, A549 and Jurkat cell lysates. IHC: Human ovary, small intestine, lymph node, spleen and stomach tissues. |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, High Performance, Validated |
| Product Description | Mouse anti Human Survivin Antibody, Monoclonal (C3), could be used for WB, IHC and so on. Application WB: 0.01-2 µg/mL IHC: 5-30 μg/mL Protein Function Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May play a role in neoplasia. May counteract a default induction of apoptosis in G2/M phase. Inhibitor of caspase-3 and caspase-7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform. |
| Isotype | Mouse IgG1 |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A + Protein G purified |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Survivin Mouse mAb (Ab129838) - Western Blot
All lanes: Survivin Mouse mAb (Ab129838) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/30000 dilution
Predicted band size: 18 kDa
Observed band size: 17 kDa
Exposure time: 3.9 s
Survivin Mouse mAb (Ab129838) - IHC
DAB staining on IHC
Sample: Human Uterus Tissue
Primary Ab: 30 μg/mL Survivin Mouse mAb (Ab129838)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
Survivin Mouse mAb (Ab129838) - IHC
DAB staining on IHC
Sample: Human Ovary Tissue
Primary Ab: 30 μg/mL Survivin Mouse mAb (Ab129838)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
Survivin Mouse mAb (Ab129838) - IHC
DAB staining on IHC
Sample: Human Small intestine Tissue
Primary Ab: 30 μg/mL Survivin Mouse mAb (Ab129838)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
Survivin Mouse mAb (Ab129838) - IHC
DAB staining on IHC
Sample: Human Lymph node Tissue
Primary Ab: 30 μg/mL Survivin Mouse mAb (Ab129838)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
Survivin Mouse mAb (Ab129838) - IHC
DAB staining on IHC
Sample: Human Spleen Tissue
Primary Ab: 30 μg/mL Survivin Mouse mAb (Ab129838)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
Survivin Mouse mAb (Ab129838) - IHC
DAB staining on IHC
Sample: Human Stomach Tissue
Primary Ab: 30 μg/mL Survivin Mouse mAb (Ab129838)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| IHC | 5-30 µg/mL |
| WB |
0.01-2 µg/mL
|
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F1202056 | Certificate of Analysis | Dec 14, 2023 | Ab129838 |
| ZJ23F1202061 | Certificate of Analysis | Dec 14, 2023 | Ab129838 |
| ZJ23F1202060 | Certificate of Analysis | Dec 14, 2023 | Ab129838 |
| 1. S K Knauer,U-R Heinrich,C Bier,N Habtemichael,D Docter,K Helling,W J Mann,R H Stauber. (2011-03-03) An otoprotective role for the apoptosis inhibitor protein survivin.. Cell death & disease, 1 (e51-e51). [PMID:21364656] |
| 2. Somsundaram N Chettiar, James V Cooley, In-Hee Park, Deepak Bhasin, Arnab Chakravarti, Pui-Kai Li, Chenglong Li, Naduparambil Korah Jacob,. (2013-08-24) Design, synthesis and biological studies of survivin dimerization modulators that prolong mitotic cycle.. Bioorganic & medicinal chemistry letters, 23 ((19)): ( 5429-5433 ). [PMID:23968825] |
| 3. Abadi AH, Abouel-Ella DA, Lehmann J, Tinsley HN, Gary BD, Piazza GA, Abdel-Fattah MA.. (2010) Discovery of colon tumor cell growth inhibitory agents through a combinatorial approach.. Eur J Med Chem, 45 (1): (90-97). [PMID:19836860] |
| 4. Mandal PK, Gao F, Lu Z, Ren Z, Ramesh R, Birtwistle JS, Kaluarachchi KK, Chen X, Bast RC, Liao WS, McMurray JS.. (2011) Potent and selective phosphopeptide mimetic prodrugs targeted to the Src homology 2 (SH2) domain of signal transducer and activator of transcription 3.. J Med Chem, 54 (10): (3549-3563). [PMID:21486047] |
| 5. Carta D, Bortolozzi R, Hamel E, Basso G, Moro S, Viola G, Ferlin MG.. (2015) Novel 3-Substituted 7-Phenylpyrrolo[3,2-f]quinolin-9(6H)-ones as Single Entities with Multitarget Antiproliferative Activity.. J Med Chem, 58 (20): (7991-8010). [PMID:26418966] |
| 6. Wang Q, Arnst KE, Xue Y, Lei ZN, Ma D, Chen ZS, Miller DD, Li W.. (2018) Synthesis and biological evaluation of indole-based UC-112 analogs as potent and selective survivin inhibitors.. Eur J Med Chem, 149 (211-224). [PMID:29501942] |
| 7. Ambrosini, G G, Adida, C C and Altieri, D C DC.. (1997) A novel anti-apoptosis gene, survivin, expressed in cancer and lymphoma.. Nature medicine, [PMID:9256286] |
| 8. Li, F F and 6 more authors.. (1998) Control of apoptosis and mitotic spindle checkpoint by survivin.. Nature, (10): [PMID:9859993] |
| 9. Mahotka, C C, Wenzel, M M, Springer, E E, Gabbert, H E HE and Gerharz, C D CD.. (1999) Survivin-deltaEx3 and survivin-2B: two novel splice variants of the apoptosis inhibitor survivin with different antiapoptotic properties.. Cancer research, (15): [PMID:10626797] |
| 10. Verdecia, M A MA and 5 more authors.. (2000) Structure of the human anti-apoptotic protein survivin reveals a dimeric arrangement.. Nature structural biology, [PMID:10876248] |
| 11. Chantalat, L L and 5 more authors.. (2000) Crystal structure of human survivin reveals a bow tie-shaped dimer with two unusual alpha-helical extensions.. Molecular cell, [PMID:10949039] |
| 12. O'Connor, D S DS and 8 more authors.. (2000) Regulation of apoptosis at cell division by p34cdc2 phosphorylation of survivin.. Proceedings of the National Academy of Sciences of the United States of America, (21): [PMID:11069302] |
| 13. Uren, A G AG and 6 more authors.. (2000) Survivin and the inner centromere protein INCENP show similar cell-cycle localization and gene knockout phenotype.. Current biology : CB, (2): [PMID:11084331] |
| 14. Marusawa, Hiroyuki H and 6 more authors.. (2003) HBXIP functions as a cofactor of survivin in apoptosis suppression.. The EMBO journal, (2): [PMID:12773388] |
| 15. Wheatley, Sally P SP, Henzing, Alexander J AJ, Dodson, Helen H, Khaled, Walid W and Earnshaw, William C WC.. (2004) Aurora-B phosphorylation in vitro identifies a residue of survivin that is essential for its localization and binding to inner centromere protein (INCENP) in vivo.. The Journal of biological chemistry, (13): [PMID:14610074] |
| 16. Badran, Adel A and 6 more authors.. (2004) Identification of a novel splice variant of the human anti-apoptopsis gene survivin.. Biochemical and biophysical research communications, (13): [PMID:14741722] |
| 17. Gassmann, Reto R and 8 more authors.. (2004) Borealin: a novel chromosomal passenger required for stability of the bipolar mitotic spindle.. The Journal of cell biology, (19): [PMID:15249581] |
| 18. Samuel, Temesgen T and 5 more authors.. (2005) cIAP1 Localizes to the nuclear compartment and modulates the cell cycle.. Cancer research, (1): [PMID:15665297] |
| 19. Vader, Gerben G, Kauw, Jos J W JJ, Medema, René H RH and Lens, Susanne M A SM.. (2006) Survivin mediates targeting of the chromosomal passenger complex to the centromere and midbody.. EMBO reports, [PMID:16239925] |
| 20. Noton, Elizabeth A EA and 6 more authors.. (2006) Molecular analysis of survivin isoforms: evidence that alternatively spliced variants do not play a role in mitosis.. The Journal of biological chemistry, (13): [PMID:16291752] |
| 21. Vong, Queenie P QP, Cao, Kan K, Li, Hoi Y HY, Iglesias, Pablo A PA and Zheng, Yixian Y.. (2005) Chromosome alignment and segregation regulated by ubiquitination of survivin.. Science (New York, N.Y.), (2): [PMID:16322459] |
| 22. Zheng, Wenyun W and 5 more authors.. (2005) Molecular cloning and bioinformatics analysis of a novel spliced variant of survivin from human breast cancer cells.. DNA sequence : the journal of DNA sequencing and mapping, [PMID:16329164] |
| 23. Wheatley, Sally P SP and McNeish, Iain A IA.. (2005) Survivin: a protein with dual roles in mitosis and apoptosis.. International review of cytology, [PMID:16344111] |
| 24. Chang, Junn-Liang JL and 7 more authors.. (2006) Borealin/Dasra B is a cell cycle-regulated chromosomal passenger protein and its nuclear accumulation is linked to poor prognosis for human gastric cancer.. Experimental cell research, (15): [PMID:16427043] |
| 25. Lens, Susanne M A SM and 5 more authors.. (2006) Uncoupling the central spindle-associated function of the chromosomal passenger complex from its role at centromeres.. Molecular biology of the cell, [PMID:16436504] |
| 26. Zody, Michael C MC and 73 more authors.. (2006) DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage.. Nature, (20): [PMID:16625196] |
| 27. Cao, Lihuan L and 7 more authors.. (2006) Survivin mutant (Surv-DD70, 71AA) disrupts the interaction of Survivin with Aurora B and causes multinucleation in HeLa cells.. Biochemical and biophysical research communications, (28): [PMID:16762323] |
| 28. Faitar, Silviu L SL, Sossey-Alaoui, Khalid K, Ranalli, Tamara A TA and Cowell, John K JK.. (2006) EVI5 protein associates with the INCENP-aurora B kinase-survivin chromosomal passenger complex and is involved in the completion of cytokinesis.. Experimental cell research, (15): [PMID:16764853] |
| 29. Jeyaprakash, A Arockia AA and 5 more authors.. (2007) Structure of a Survivin-Borealin-INCENP core complex reveals how chromosomal passengers travel together.. Cell, (19): [PMID:17956729] |
| 30. Gagarina, Viktoria V, Carlberg, Alyssa L AL, Pereira-Mouries, Lucilia L and Hall, David J DJ.. (2008) Cartilage oligomeric matrix protein protects cells against death by elevating members of the IAP family of survival proteins.. The Journal of biological chemistry, (4): [PMID:17993464] |
| 31. Pohl, Christian C and Jentsch, Stefan S.. (2008) Final stages of cytokinesis and midbody ring formation are controlled by BRUCE.. Cell, (7): [PMID:18329369] |
| 32. Xia, Fang F, Canovas, Pedro M PM, Guadagno, Thomas M TM and Altieri, Dario C DC.. (2008) A survivin-ran complex regulates spindle formation in tumor cells.. Molecular and cellular biology, [PMID:18591255] |
| 33. Daub, Henrik H and 9 more authors.. (2008) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle.. Molecular cell, (8): [PMID:18691976] |
| 34. Bourhis, Eric E, Lingel, Andreas A, Phung, Qui Q, Fairbrother, Wayne J WJ and Cochran, Andrea G AG.. (2009) Phosphorylation of a borealin dimerization domain is required for proper chromosome segregation.. Biochemistry, (28): [PMID:19530738] |
| 35. Habtemichael, Negusse N and 10 more authors.. (2010) Expression analysis suggests a potential cytoprotective role of Birc5 in the inner ear.. Molecular and cellular neurosciences, [PMID:20627126] |
| 36. Wang, Haijuan H and 8 more authors.. (2010) Acetylation directs survivin nuclear localization to repress STAT3 oncogenic activity.. The Journal of biological chemistry, (12): [PMID:20826784] |
| 37. Yamagishi, Yuya Y, Honda, Takashi T, Tanno, Yuji Y and Watanabe, Yoshinori Y.. (2010) Two histone marks establish the inner centromere and chromosome bi-orientation.. Science (New York, N.Y.), (8): [PMID:20929775] |
| 38. Platica, Micsunica M and 5 more authors.. (2011) PAR, a protein involved in the cell cycle, is functionally related to chromosomal passenger proteins.. International journal of oncology, [PMID:21225229] |
| 39. Barrett, Rachel M A RM, Colnaghi, Rita R and Wheatley, Sally P SP.. (2011) Threonine 48 in the BIR domain of survivin is critical to its mitotic and anti-apoptotic activities and can be phosphorylated by CK2 in vitro.. Cell cycle (Georgetown, Tex.), (1): [PMID:21252625] |
| 40. Pavlyukov, Marat S MS and 5 more authors.. (2011) Survivin monomer plays an essential role in apoptosis regulation.. The Journal of biological chemistry, (1): [PMID:21536684] |
| 41. Li, Zhijun Z and 6 more authors.. (2014) CUL9 mediates the functions of the 3M complex and ubiquitylates survivin to maintain genome integrity.. Molecular cell, (5): [PMID:24793696] |
| 42. Park, Mi Hee MH, Kim, Soo Yeon SY, Kim, Young Ju YJ and Chung, Young-Ho YH.. (2014) ALS2CR7 (CDK15) attenuates TRAIL induced apoptosis by inducing phosphorylation of survivin Thr34.. Biochemical and biophysical research communications, (18): [PMID:24866247] |
| 43. Liu, Yuan Y and 8 more authors.. (2015) The Proapoptotic F-box Protein Fbxl7 Regulates Mitochondrial Function by Mediating the Ubiquitylation and Proteasomal Degradation of Survivin.. The Journal of biological chemistry, (8): [PMID:25778398] |
| 44. Sasai, Kaori K, Katayama, Hiroshi H, Hawke, David H DH and Sen, Subrata S.. (2016) Aurora-C Interactions with Survivin and INCENP Reveal Shared and Distinct Features Compared with Aurora-B Chromosome Passenger Protein Complex.. PloS one, [PMID:27332895] |
| 45. Dheekollu, Jayaraju and 7 more authors.. (2017) Carcinoma-risk variant of EBNA1 deregulates Epstein-Barr Virus episomal latency.. Oncotarget, (31): [PMID:28077791] |
| 46. Kamran, M M and 8 more authors.. (2017) Aurora kinase A regulates Survivin stability through targeting FBXL7 in gastric cancer drug resistance and prognosis.. Oncogenesis, (20): [PMID:28218735] |
