TWEEN® 20 - Suitable for molecular biology, viscous liquids, high purity , CAS No.9005-64-5

Item Number
T434505
Grouped product items
SKUSizeAvailabilityPrice Qty
T434505-50ml
50ml
In stock
$200.90
T434505-100ml
100ml
In stock
$366.90

Basic Description

SynonymsPOE (6) sorbitol monolaurate | Tween(R) 20 | 2-{2-[3,4-bis(2-hydroxyethoxy)oxolan-2-yl]-2-(2-hydroxyethoxy)ethoxy}ethyl dodecanoate | SCHEMBL118527 | Tween 40 (TN) | 1052273-76-3 | 2-(2-(3,4-bis(2-hydroxyethoxy)tetrahydrofuran-2-yl)-2-(2-hydroxyethoxy)eth
Specifications & Purityfor molecular biology, viscous liquids
Legal InformationTWEEN is a registered trademark of Croda International PLC
Gradefor molecular biology
Note注意: TWEEN 20在高压灭菌下可能会不稳定,特别是在含有金属阳离子的缓冲溶液中。不推荐在没有进行属性改变测试的情况下进行高压灭菌。TWEEN 20对热较为敏感并在暴露于升高的温度下时会变暗。据报道,聚山梨醇酯与碱、重金属盐、酚和单宁酸不相容。它们还可能会降低多种保存剂的活性。聚山梨醇酯的水溶液在储存期间会经历自动氧化,以及由光、温度升高和硫酸铜所催化带来的改变。
Product Description

ECO TWEEN® 20, also known as Polyoxyethylenesorbitan monolaurate, is a bio-based, nonionic detergent manufactured from a natural fatty acid. This surfactant, derived from natural sources, provides various functional advantages, such as detergency, emulsification, and wetting. Its versatility makes it well-suited for a variety of applications in biochemical and biological research, with common uses including cell lysis, protein extraction, nuclei isolation and cell fractionation. We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Green Chemistry. This product is a biobased surfactant and is aligned with the 7th principle of Green Chemistry "Use of Renewable Feedstocks" and the 10th principle "Design for Degradation".
TWEEN 20 is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached.

Mechanisms of Action

Mechanism of ActionAction Typetarget IDTarget NameTarget TypeTarget OrganismBinding Site NameReferences

Names and Identifiers

IUPAC Name 2-[2-[3,4-bis(2-hydroxyethoxy)oxolan-2-yl]-2-(2-hydroxyethoxy)ethoxy]ethyl dodecanoate
INCHI InChI=1S/C26H50O10/c1-2-3-4-5-6-7-8-9-10-11-24(30)34-19-18-31-20-22(32-15-12-27)26-25(35-17-14-29)23(21-36-26)33-16-13-28/h22-23,25-29H,2-21H2,1H3
InChi Key HMFKFHLTUCJZJO-UHFFFAOYSA-N
Canonical SMILES CCCCCCCCCCCC(=O)OCCOCC(C1C(C(CO1)OCCO)OCCO)OCCO
Isomeric SMILES CCCCCCCCCCCC(=O)OCCOCC(C1C(C(CO1)OCCO)OCCO)OCCO
WGK Germany 1
RTECS TR7400000
PubChem CID 443314

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

5 results found

Lot NumberCertificate TypeDateItem
C2425038Certificate of AnalysisMar 29, 2024 T434505
F23161117Certificate of AnalysisJun 20, 2023 T434505
F23161120Certificate of AnalysisJun 20, 2023 T434505
C2310592Certificate of AnalysisMar 23, 2023 T434505
C2310605Certificate of AnalysisMar 23, 2023 T434505

Chemical and Physical Properties

Refractive Index1.4685-1.4715
Flash Point(°C)>149℃
Boil Point(°C)100°C
Melt Point(°C)-14 °C

Safety and Hazards(GHS)

WGK Germany 1
RTECS TR7400000

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143. Yuting Gao, Yan Wu, Pengcheng Huang, Fang-Ying Wu.  (2022)  Carbon Dot-Encapsulated Plasmonic Core-Satellite Nanoprobes for Sensitive Detection of Cancer Biomarkers via Dual-Mode Colorimetric and Fluorometric Immunoassay.  ACS Applied Nano Materials,  (8): (11539–11548).  [PMID:] [10.1021/acsanm.2c02554]
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145. Lei Zhao, Ziyun Ye, Qilu Xue, Langlang Yi, Guoqian Li, Bo Hu, Ruichan Lv, Jie Tian.  (2022)  A plug-and-play 3D hydrodynamic focusing Raman platform for label-free and dynamic single microparticle detection.  SENSORS AND ACTUATORS B-CHEMICAL,  369  (132273).  [PMID:] [10.1016/j.snb.2022.132273]
146. Yuanyuan Liu, Bangyan Liu, Lian Xia, Hongyan Yu, Qiaodie Wang, Yuangen Wu.  (2022)  Cationic polyelectrolyte as powerful capture molecule in aptamer-based chromatographic strip for rapid visual detection of paraquat residue in agricultural products.  SENSORS AND ACTUATORS B-CHEMICAL,  368  (132237).  [PMID:] [10.1016/j.snb.2022.132237]
147. Shusheng Wu, Yuye Yin, Longfei Du.  (2022)  FUS aggregation following ischemic stroke favors brain astrocyte activation through inducing excessive autophagy.  EXPERIMENTAL NEUROLOGY,  355  (114144).  [PMID:35718207] [10.1016/j.expneurol.2022.114144]
148. Chunyong Song, Yufeng Lin, Pengzhi Hong, Huanming Liu, Chunxia Zhou.  (2022)  Compare with different vegetable oils on the quality of the Nemipterus virgatus surimi gel.  Food Science & Nutrition,  10  (9): (2935-2946).  [PMID:36171767] [10.1002/fsn3.2889]
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150. Liyu Jiang, Yan Zeng, Leilei Ai, Hao Yan, Xiaochun Yang, Peihua Luo, Bo Yang, Zhifei Xu, Qiaojun He.  (2022)  Decreased HMGB1 expression contributed to cutaneous toxicity caused by lapatinib.  BIOCHEMICAL PHARMACOLOGY,  201  (115105).  [PMID:35617997] [10.1016/j.bcp.2022.115105]
151. Lu Zheng, Hua Wang, Peng Zuo, Yueling Liu, Huiying Xu, Bang-Ce Ye.  (2022)  Rapid On-Chip Isolation of Cancer-Associated Exosomes and Combined Analysis of Exosomes and Exosomal Proteins.  ANALYTICAL CHEMISTRY,  94  (21): (7703–7712).  [PMID:35575685] [10.1021/acs.analchem.2c01187]
152. Yupeng Guan, Chengyuan Xing, Tongyu Tong, Xinyi Zhang, Jun Li, Huikun Chen, Junfeng Zhu, Yang Kang, Jun Pang.  (2022)  Smart dual responsive nanocarriers with reactive oxygen species amplification assisted synergistic chemotherapy against prostate cancer.  JOURNAL OF COLLOID AND INTERFACE SCIENCE,  622  (789).  [PMID:35544966] [10.1016/j.jcis.2022.04.167]
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155. Li Ji, Leheng Zhang, Huiyi Yang, Shan Liang, Junkang Pan, Yikui Zou, Shijia Li, Qinglan Li, Suqing Zhao.  (2022)  Versatile Au@Ru nanocomposites for the rapid detection of Salmonella typhimurium and photothermal sterilization.  JOURNAL OF COLLOID AND INTERFACE SCIENCE,  621  (489).  [PMID:35483180] [10.1016/j.jcis.2022.04.028]
156. Xiaojuan Han, Yuan Liu, Yan Dai, Tianshu Xu, Qinghui Hu, Xiaolan Yi, Liangyou Rui, Gang Hu, Jun Hu.  (2022)  Neuronal SH2B1 attenuates apoptosis in an MPTP mouse model of Parkinson's disease via promoting PLIN4 degradation.  Redox Biology,  52  (102308).  [PMID:35390677] [10.1016/j.redox.2022.102308]
157. Jiaxin Chen, Chuanai Cao, Dongxue Yuan, Xiufang Xia, Qian Liu, Baohua Kong.  (2022)  Impact of different ionic strengths on protein-lipid co-oxidation in whey protein isolate-stabilized oil-in-water emulsions.  FOOD CHEMISTRY,  385  (132700).  [PMID:35305436] [10.1016/j.foodchem.2022.132700]
158. Yuan Shuai, Zhou Mingyong, Peng Tao, Li Qiang, Jiang Fengze.  (2022)  An investigation of chaotic mixing behavior in a planar microfluidic mixer.  PHYSICS OF FLUIDS,  34  (3):   [PMID:] [10.1063/5.0082831]
159. Liu Jing-yi, Wu Shi-zhao, Shen Zhu, Gao Jing, Hu Xin-quan, Li Guo-hua.  (2022)  Enhanced anticorrosion property of epoxy resin membrane by nano-organic montmorillonite.  Journal of Coatings Technology and Research,  19  (4): (1087-1100).  [PMID:] [10.1007/s11998-021-00587-6]
160. Pengcheng Wu, Feng Xue, Wanchao Zuo, Jun Yang, Xinmei Liu, Hui Jiang, Jianjun Dai, Yanmin Ju.  (2022)  A Universal Bacterial Catcher Au–PMBA-Nanocrab-Based Lateral Flow Immunoassay for Rapid Pathogens Detection.  ANALYTICAL CHEMISTRY,  94  (10): (4277–4285).  [PMID:35244383] [10.1021/acs.analchem.1c04909]
161. Shan Liang, Li Ji, Yingying Zhong, Tiantian Wang, Huiyi Yang, Qing-Lan Li, Xiangguang Li, Suqing Zhao.  (2022)  Fluorescence immunoassay for the targeted determination of trace Listeria monocytogenes based on immunomagnetic separation and CdZnTe quantum dot indication.  Analytical Methods,  14  (11): (1124-1133).  [PMID:35212322] [10.1039/D1AY02106K]
162. Lijiao Ao, Tao Liao, Liang Huang, Shan Lin, Kui Xu, Jiangtao Ma, Shaorong Qiu, Xiangyu Wang, Qiqing Zhang.  (2022)  Sensitive and simultaneous detection of multi-index lung cancer biomarkers by an NIR-Ⅱ fluorescence lateral-flow immunoassay platform.  CHEMICAL ENGINEERING JOURNAL,  436  (135204).  [PMID:] [10.1016/j.cej.2022.135204]
163. Chunyong Song, Yufeng Lin, Pengzhi Hong, Huanming Liu, Chunxia Zhou.  (2022)  Low-Content Pre-Emulsified Safflower Seed Oil Enhances the Quality and Flavor of the Nemipterus Virgatus Surimi Gel.  Gels,  (2): (106).  [PMID:35200487] [10.3390/gels8020106]
164. Zhanwei Liang, Tao Peng, Xueshima Jiao, Yang Zhao, Jie Xie, You Jiang, Bo Meng, Xiang Fang, Xiaoping Yu, Xinhua Dai.  (2022)  Latex Microsphere-Based Bicolor Immunochromatography for Qualitative Detection of Neutralizing Antibody against SARS-CoV-2.  Biosensors-Basel,  12  (2): (103).  [PMID:35200362] [10.3390/bios12020103]
165. Piao Mingyue, Du Hongxue, Sun Yuwei, Teng Honghui.  (2022)  Self-regeneration hybrid hydrogel for bisphenol a adsorption in water.  ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH,  29  (28): (43169-43178).  [PMID:35092593] [10.1007/s11356-022-18833-8]
166. Chenyang Zhao, Yujia Liu, Hongwei Zhu, Junjie Feng, Huiyun Jiang, Fei An, Yan Jin, Wei Xu, Zhe Yang, Bing Sun.  (2022)  Hydrophobically modified Pd membrane for the efficient purification of hydrogen in light alcohols steam reforming process.  JOURNAL OF MEMBRANE SCIENCE,  647  (120326).  [PMID:] [10.1016/j.memsci.2022.120326]
167. Yanling Zhao, Yanfei Wen, Xing Hu, Bing Zhang.  (2022)  A Colorimetric Immunoassay Based on g-C3N4@Fe3O4 Nanocomposite for Detection of Carcinoembryonic Antigen.  Journal of Analytical Methods in Chemistry,  2022  (6966470).  [PMID:35127195] [10.1155/2022/6966470]
168. Chuanqi Wang, Manting Wang, Peng Chen, Jiexin Wang, Yuan Le.  (2022)  Dasatinib Nanoemulsion and Nanocrystal for Enhanced Oral Drug Delivery.  Pharmaceutics,  14  (1): (197).  [PMID:35057093] [10.3390/pharmaceutics14010197]
169. Guo Shuai, Wei Chaoxian, Zhu Yaxian, Zhang Yong.  (2022)  How surfactants affect the depuration of polycyclic aromatic hydrocarbons adsorbed on the mangrove leaf surfaces: insight from an in situ method.  ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH,  29  (21): (31413-31425).  [PMID:35001285] [10.1007/s11356-021-18469-0]
170. Liya Zeng, Zhe Wang.  (2022)  Development of an environmentally friendly nanoemulsion based on Ginkgo biloba exocarp extracts to control Plutella xylostella.  Micro & Nano Letters,  17  (2): (42-48).  [PMID:] [10.1049/mna2.12104]
171. Tao Peng, Xueshima Jiao, Zhanwei Liang, Hongwei Zhao, Yang Zhao, Jie Xie, You Jiang, Xiaoping Yu, Xiang Fang, Xinhua Dai.  (2022)  Lateral Flow Immunoassay Coupled with Copper Enhancement for Rapid and Sensitive SARS-CoV-2 Nucleocapsid Protein Detection.  Biosensors-Basel,  12  (1): (13).  [PMID:35049641] [10.3390/bios12010013]
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174. Liye Wang, Jiangxiu Niu, Pengyuan Wei, Yilei Feng, Mingyue Ding, Chaojun He, Yongjie Ma, Yaolei Zhu, Jia Li, Lingli Huang, Xiya Zhang.  (2022)  Rapid determination of 2,4-diaminopyrimidine residues through sample pretreatment using immunomagnetic bead purification along with HPLC–UV.  FOOD CHEMISTRY,  376  (131835).  [PMID:34980530] [10.1016/j.foodchem.2021.131835]
175. Liya Zeng, Yongchang Liu, Zhihui Yuan, Zhe Wang.  (2021)  Formation and Physical Stability of Zanthoxylum bungeanum Essential Oil Based Nanoemulsions Co-Stabilized with Tea Saponin and Synthetic Surfactant.  MOLECULES,  26  (24): (7464).  [PMID:34946544] [10.3390/molecules26247464]
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177. Lv Xianqing, Yang Guangxu, Gong Zhenggang, Cheng Xin, Shuai Li, Huang Liulian, Chen Lihui, Luo Xiaolin, Liu Jing.  (2021)  Using poly(N-Vinylcaprolactam) to Improve the Enzymatic Hydrolysis Efficiency of Phenylsulfonic Acid-Pretreated Bamboo.  Frontiers in Bioengineering and Biotechnology,    [PMID:34917604] [10.3389/fbioe.2021.804456]
178. Yuyang Zhou, Xiaochun Wu, Jin Zhang, Zhenxing Wang.  (2022)  In situ formation of tannic (TA)-aminopropyltriethoxysilane (APTES) nanospheres on inner and outer surface of polypropylene membrane toward enhanced dye removal capacity.  CHEMICAL ENGINEERING JOURNAL,  433  (133843).  [PMID:] [10.1016/j.cej.2021.133843]
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183. Juanjuan Huang, Xianfeng Chen, Aihua Xie.  (2021)  Formononetin ameliorates IL‑13‑induced inflammation and mucus formation in human nasal epithelial cells by activating the SIRT1/Nrf2 signaling pathway.  Molecular Medicine Reports,  24  (6): (1-9).  [PMID:34590155] [10.3892/mmr.2021.12472]
184. Xianlong Zhang, Guoliang Li, Jianghua Liu, Zhuoqun Su.  (2021)  Bio-inspired Nanoenzyme Synthesis and Its Application in A Portable Immunoassay for Food Allergy Proteins.  JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,  69  (49): (14751–14760).  [PMID:34523915] [10.1021/acs.jafc.1c04309]
185. Yinrui Guo, Hongya Guo, Lingyan Qiu, Yuanfei Fu, Xiangxiang Zhu, Haiting Zhang, Jian Wang, Diling Chen.  (2021)  Appetite Suppression and Interleukin 17 Receptor Signaling Activation of Colonic Mycobiota Dysbiosis Induced by High Temperature and High Humidity Conditions.  Frontiers in Cellular and Infection Microbiology,  11  (657807).  [PMID:34568080] [10.3389/fcimb.2021.657807]
186. Chenchen Ge, Juan Feng, Jiaming Zhang, Kai Hu, Dou Wang, Ling Zha, Xuejuan Hu, Rongsong Li.  (2022)  Aptamer/antibody sandwich method for digital detection of SARS-CoV2 nucleocapsid protein.  TALANTA,  236  (122847).  [PMID:34635237] [10.1016/j.talanta.2021.122847]
187. Wang Meng, Liu Haoyu, Zhang Xu, Zhao Wenbo, Lin Xiaoyan, Zhang Fei, Li Danyang, Xu Chengpeng, Xie Fei, Wu Zhen, Yang Qibing, Li Xiangzhi.  (2021)  Lack of MOF Decreases Susceptibility to Hypoxia and Promotes Multidrug Resistance in Hepatocellular Carcinoma via HIF-1α.  Frontiers in Cell and Developmental Biology,  (2439).  [PMID:34540836] [10.3389/fcell.2021.718707]
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189. Kai-Ren Zhao, Li Wang, Peng-Fei Liu, Xiao-Min Hang, Hui-Yi Wang, Shu-Ying Ye, Zhi-Jun Liu, Guo-Xi Liang.  (2021)  A signal-switchable electrochemiluminescence biosensor based on the integration of spherical nucleic acid and CRISPR/Cas12a for multiplex detection of HIV/HPV DNAs.  SENSORS AND ACTUATORS B-CHEMICAL,  346  (130485).  [PMID:] [10.1016/j.snb.2021.130485]
190. Yang Zuoyi, Mao Xingchen, Cui Jiahao, Wang Yujie, Zhang Yaping.  (2021)  Enhancement and analysis of Anthracene degradation by Tween 80 in LMS-HOBt.  Scientific Reports,  11  (1): (1-11).  [PMID:34162899] [10.1038/s41598-021-90609-5]
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192. Guo Yinrui, Zhu Xiangxiang, Zeng Miao, Qi Longkai, Tang Xiaocui, Wang Dongdong, Zhang Mei, Xie Yizhen, Li Hongye, Yang Xin, Chen Diling.  (2021)  A diet high in sugar and fat influences neurotransmitter metabolism and then affects brain function by altering the gut microbiota.  Translational Psychiatry,  11  (1): (1-27).  [PMID:34045460] [10.1038/s41398-021-01443-2]
193. Liang Huang, Yuxing Zhang, Tao Liao, Kui Xu, Chenxing Jiang, Dinglv Zhuo, Yang Wang, Hui-Min Wen, Jing Wang, Lijiao Ao, Jun Hu.  (2021)  Compact Magneto-Fluorescent Colloids by Hierarchical Assembly of Dual-Components in Radial Channels for Sensitive Point-of-Care Immunoassay.  Small,  17  (25): (2100862).  [PMID:34032374] [10.1002/smll.202100862]
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195. Tingting Xu, Pei Wang, Xue Zheng, Zhanpeng Yan, Kun Li, Jindi Xu, Cuihua Jiang, Fangshi Zhu.  (2021)  The therapeutic effects and mechanisms of Long Chai Fang on chronic hepatitis B.  Annals of Translational Medicine,  (10): (865).  [PMID:34164499] [10.21037/atm-21-1923]
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198. Sizhu Tian, Xuwen Li, Jia Jiang, Li Tang, Hanqi Zhang, Yong Yu, Ziwei Zhang.  (2021)  Highly sensitive detection of rabbit IgG by electron spin resonance using CuS nanoparticles as probe.  SENSORS AND ACTUATORS B-CHEMICAL,  338  (129835).  [PMID:] [10.1016/j.snb.2021.129835]
199. Danqiu Luo, Xiaohuan Huang, Bangyan Liu, Wenying Zou, Yuangen Wu.  (2021)  Facile Colorimetric Nanozyme Sheet for the Rapid Detection of Glyphosate in Agricultural Products Based on Inhibiting Peroxidase-Like Catalytic Activity of Porous Co3O4 Nanoplates.  JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,  69  (11): (3537–3547).  [PMID:33721998] [10.1021/acs.jafc.0c08208]
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201. Zeyang Liu, Hongyong Zhang, Zhen Zhan, Haochen Nan, Nan Huang, Tao Xu, Xiaohua Gong, Chengzhi Hu.  (2021)  Mild formation of core–shell hydrogel microcapsules for cell encapsulation.  Biofabrication,  13  (2): (25002).  [PMID:33271516] [10.1088/1758-5090/abd076]
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207. Tao Peng, Zhiwei Sui, Zhenghui Huang, Jie Xie, Kai Wen, Yongzhuo Zhang, Wenfeng Huang, Wei Mi, Ke Peng, Xinhua Dai, Xiang Fang.  (2021)  Point-of-care test system for detection of immunoglobulin-G and -M against nucleocapsid protein and spike glycoprotein of SARS-CoV-2.  SENSORS AND ACTUATORS B-CHEMICAL,  331  (129415).  [PMID:33519091] [10.1016/j.snb.2020.129415]
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Solution Calculators