Taq PCR Mix - 25μl /T, high purity

Item Number

T283589

• Specifications & Purity: 25μl /T
• Storage Temp: Store at -20°C
• Shipped In: Ice chest + Ice pads
• Grade: EnzymoPure™

Product Description

Product manual 

This reagent provides a simple and sensitive PCR detection system. The core reagent contains optimized buffer, dNTPs Mix, common Taq enzyme (Taq DNA polymerase) and Mg 2+ (final concentration 2mM) solution. Users only need to add appropriate primers and probes or fluorescent dyes to proceed. Fluorescence PCR detection.

Instructions

Please pay attention to mixing before use, and then prepare a 50μl PCR reaction solution of the reaction system according to the following components

Reagent	Volume	Final concentration
Taq PCR Mix(2×)	25μl	1×
Primer-probe Mix	3μl	—
Template	10μl	—
Ultra-pure water	Up to 50μl	—
Total capacity	50μl	—

Note: The amount of primers, probes and templates can be adjusted by yourself.

Experimental setup

Step	Temperature	Time	Number of cycles
1	95℃	2.5min	1
2	94℃	15s	40
3	55℃	40s

Note: The collection of fluorescence is set to two or three steps and the conditions can be set according to your experimental settings.