This reagent provides a simple and sensitive PCR detection system. The core reagent contains optimized buffer, dNTPs Mix, common Taq enzyme (Taq DNA polymerase) and Mg 2+ (final concentration 2mM) solution. Users only need to add appropriate primers and probes or fluorescent dyes to proceed. Fluorescence PCR detection.
Instructions
Please pay attention to mixing before use, and then prepare a 50μl PCR reaction solution of the reaction system according to the following components
Reagent
Volume
Final concentration
Taq PCR Mix(2×)
25μl
1×
Primer-probe Mix
3μl
—
Template
10μl
—
Ultra-pure water
Up to 50μl
—
Total capacity
50μl
—
Note: The amount of primers, probes and templates can be adjusted by yourself.
Experimental setup
Step
Temperature
Time
Number of cycles
1
95℃
2.5min
1
2
94℃
15s
40
3
55℃
40s
Note: The collection of fluorescence is set to two or three steps and the conditions can be set according to your experimental settings.
