pAb; Rabbit anti Human TGIF2 Antibody; WB, IHC; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab130920-10μl | 10μl | In stock | $79.90 | |
| Ab130920-50μl | 50μl | In stock | $239.90 | |
| Ab130920-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $399.90 | |
| Ab130920-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,699.90 |
pAb; Rabbit anti Human TGIF2 Antibody; WB, IHC; Unconjugated
| Product Name | TGIF2 Antibody - Primary antibody, specific to TGIF2, Rabbit IgG |
|---|---|
| Synonyms | TGIF2 | 5' TG 3' interacting factor 2 antibody | 5''-TG-3''-interacting factor 2 antibody | Homeobox protein TGIF2 antibody | TGF (beta) induced transcription factor 2 antibody | TGF beta induced factor 2 antibody | TGF-beta-induced transcription factor 2 |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, 0.5 mg/mL |
| Host species | Rabbit |
| Specificity | TGIF2 |
| Immunogen | A KLH conjugated synthetic peptide from the C-terminal region of human TGIF2 (AA 171-199) |
| Positive Control | WB: HeLa, SK-OV-3 and A431 cell lysates. IHC: Human skin tissue. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description | Rabbit anti Human TGIF2 Antibody, Polyclonal, could be used for WB, IHC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified+ Peptide affinity purified |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
TGIF2 Antibody (Ab130920) - Western Blot
All lanes: TGIF2 Antibody (Ab130920) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 26 kDa
Observed band size: 28 kDa
Exposure time: 17.9 s
TGIF2 Antibody (Ab130920) - IHC
Immunohistochemical analysis of paraffin-embedded human skin tissue using TGIF2 Antibody (Ab130920) performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH 9.0). Samples were incubated with TGIF2 Antibody (Ab130920) (1/500) for 1 hour at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB |
1/1000
|
| IHC | 1/500 |
Enter Lot Number to search for COA:
| 1. Deloukas P, Matthews LH, Ashurst J, Burton J, Gilbert JG, Jones M, Stavrides G, Almeida JP, Babbage AK, Bagguley CL, Bailey J, Barlow KF, Bates KN, Beard LM, Beare DM, Beasley OP, Bird CP, Blakey SE, Bridgeman AM, Brown AJ, Buck D, Burrill W, Butler AP, Carder C, Carter NP, Chapman JC, Clamp M, Clark G, Clark LN, Clark SY, Clee CM, Clegg S, Cobley VE, Collier RE, Connor R, Corby NR, Coulson A, Coville GJ, Deadman R, Dhami P, Dunn M, Ellington AG, Frankland JA, Fraser A, French L, Garner P, Grafham DV, Griffiths C, Griffiths MN, Gwilliam R, Hall RE, Hammond S, Harley JL, Heath PD, Ho S, Holden JL, Howden PJ, Huckle E, Hunt AR, Hunt SE, Jekosch K, Johnson CM, Johnson D, Kay MP, Kimberley AM, King A, Knights A, Laird GK, Lawlor S, Lehvaslaiho MH, Leversha M, Lloyd C, Lloyd DM, Lovell JD, Marsh VL, Martin SL, McConnachie LJ, McLay K, McMurray AA, Milne S, Mistry D, Moore MJ, Mullikin JC, Nickerson T, Oliver K, Parker A, Patel R, Pearce TA, Peck AI, Phillimore BJ, Prathalingam SR, Plumb RW, Ramsay H, Rice CM, Ross MT, Scott CE, Sehra HK, Shownkeen R, Sims S, Skuce CD, Smith ML, Soderlund C, Steward CA, Sulston JE, Swann M, Sycamore N, Taylor R, Tee L, Thomas DW, Thorpe A, Tracey A, Tromans AC, Vaudin M, Wall M, Wallis JM, Whitehead SL, Whittaker P, Willey DL, Williams L, Williams SA, Wilming L, Wray PW, Hubbard T, Durbin RM, Bentley DR, Beck S, Rogers J. (2001) The DNA sequence and comparative analysis of human chromosome 20.. Nature, 414 (6866): (865-71). [PMID:11780052] |
| 2. Imoto, I I and 5 more authors.. (2000) Amplification and overexpression of TGIF2, a novel homeobox gene of the TALE superclass, in ovarian cancer cell lines.. Biochemical and biophysical research communications, (16): [PMID:11006116] |
| 3. Melhuish, T A TA, Gallo, C M CM and Wotton, D D.. (2001) TGIF2 interacts with histone deacetylase 1 and represses transcription.. The Journal of biological chemistry, (24): [PMID:11427533] |
