Thermostable Inorganic Pyrophosphatase

Item Number

T406605

• Storage Temp: Store at -20°C
• Shipped In: Ice chest + Ice pads
• Grade: EnzymoPure™

Product Description

Inorganic pyrophosphatase (PPase) can catalyze the hydrolysis of inorganic pyrophosphate to orthophosphate: P2O74-+H2O+PPase→2HPO42-. Used in nucleic acid amplification experiments, PPase can hydrolyze the inorganic pyrophosphate generated by the reaction to avoid its inhibition of the reaction system. Under natural conditions, inorganic pyrophosphatase can provide thermodynamic power for the biosynthesis of protein, RNA and DNA, and play a role in the process of lipid metabolism, calcium absorption, and bone formation. The thermostable inorganic pyrophosphatase is purified from an inorganic pyrophosphatase gene cloned from the extremely heat-resistant Thermococus litoralis. Under high temperature conditions, the enzyme has stable properties and high enzyme activity, which meets the experimental requirements of high temperature conditions.

Features

Hydrolyzed pyrophosphate;

DNA synthesis: significantly enhance the ability of DNA replication;

RNA synthesis: increase RNA yield in in vitro transcription reaction;

Thermal stability: After heating at 100°C for 4 hours, the enzyme still has 100% activity.

Recommendations

1) The enzyme is active in a variety of reaction buffers. Usually, the enzyme can be directly added in experiments such as HDA amplification and LAMP amplification;

2) The dosage of this enzyme needs to be optimized in different experiments, usually adjusted at a concentration of 0.05~1U/ml.