A KLH conjugated synthetic peptide from the C-terminal region of human Thioredoxin 2 (AA 136-166)
Positive Control
WB: Hep G2, HeLa, K562, Jurkat, Raji, NIH/3T3 and C6 cell lysates.
Conjugation
Unconjugated
Grade
Carrier Free, ExactAb™, Validated
Product Description
Rabbit anti Human Thioredoxin 2 Antibody, Polyclonal, could be used for WB and so on. Application: WB: 1/1000 Protein Function Thioredoxin is a small redox protein found in many eukaryotes and prokaryotes. A pair of cysteines within a highly conserved, active site sequence can be oxidized to form a disulfide bond that is then reduced by thioredoxin reductase (1). Multiple forms of thioredoxin have been identified, including cytosolic thioredoxin 1 (TRX1) and mitochondrial thioredoxin 2 (TRX2). Thioredoxin participates in many cellular processes including redox signaling, response to oxidative stress, and protein reduction (1). A potential role of thioredoxin in human disorders such as cancer, aging, and heart disease is currently under investigation (2). Thioredoxin can play a key role in cancer progression, because it acts as a negative regulator of the proapoptotic kinase ASK1 (3). Changes in thioredoxin expression have been associated with meningococcal septic shock and acute lung injury (4,5).
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Saturated ammonium sulfate precipitation
Form
Liquid
Concentration
2.0 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Thioredoxin 2 Antibody (Ab130982) - Western Blot All lanes: Thioredoxin 2 Antibody (Ab130982) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 18 kDa Observed band size: 13 kDa Exposure time: 10.9 s
1.Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M. (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.. Science, 325 (5942):(834-40). [PMID:19608861]
2.Damdimopoulos AE, Miranda-Vizuete A, Pelto-Huikko M, Gustafsson JA, Spyrou G.. () Human mitochondrial thioredoxin. Involvement in mitochondrial membrane potential and cell death.. J Biol Chem, [PMID:12080052]
3.Dunham, I I and 24 more authors.. (1999) The DNA sequence of human chromosome 22.. Nature, (2):[PMID:10591208]
4.Chen, Yan Y, Cai, Jiyang J, Murphy, T J TJ and Jones, Dean P DP.. (2002) Overexpressed human mitochondrial thioredoxin confers resistance to oxidant-induced apoptosis in human osteosarcoma cells.. The Journal of biological chemistry, (6):[PMID:12032145]
5.Collins, John E JE and 12 more authors.. (2004) A genome annotation-driven approach to cloning the human ORFeome.. Genome biology, [PMID:15461802]
6.Holzerova, Eliska E and 17 more authors.. (2016) Human thioredoxin 2 deficiency impairs mitochondrial redox homeostasis and causes early-onset neurodegeneration.. Brain : a journal of neurology, [PMID:26626369]
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