Color pre dyed protein molecular weight standard: 1. This product contains 10 highly purified and pre stained recombinant proteins ranging from 10kDa to 180kDa. 2. Suitable as a protein molecular weight standard for SDS-PAGE and Western blotting. 3. This color pre stained protein molecular weight standard has been prepared in 1 × SDS-PAGE buffer and should be used directly without boiling, diluting, or adding reducing agents. 4. According to the size of the sample well, this color pre stained protein molecular weight standard usually requires 5-10 microliters of sample to be loaded each time (5μl for 5 × 1.5mm gel wells is sufficient), and very clear protein bands can be observed during electrophoresis, electrophoresis, and membrane transfer.
The pre stained protein molecular weight standard, calibrated with non pre stained protein molecular weight standards, has the following outstanding advantages: 1. The blue and green bands showed little change in apparent molecular weight on SDS-PAGE gel in Tris Glycine, Bis Tris, and Tris Acetate buffer systems. 2. The orange (red) color stripes are consistent with the changes in imported products.
Usage method: 1. After thawing at room temperature, completely dissolve and gently mix well, do not boil. 2. Take 5µl of this product and test sample for polyacrylamide gel electrophoresis at the same time; It is recommended that laboratories with conditions determine the appropriate sample size through pre experiments based on their own experimental conditions and habits when using this product for the first time. This can save costs and obtain better experimental images. 3. Unused color pre dyed protein molecular weight standards should be stored under storage conditions and can be left at 4°C for 2 months.
Matters needing attention: 1. Pay attention to the electrophoresis time, as low molecular weight proteins may migrate to the front edge of the dye. 2. During Western blotting of high molecular weight proteins, it is necessary to extend the transfer time or increase the transfer voltage. Additionally, it is recommended not to add SDS to the transfer solution. If it is necessary for the experiment, it is suggested that the SDS concentration should not exceed 0.02-0.04%. 3. Pre stained proteins have different apparent molecular weights in different buffer systems. By pre calibrating with non pre stained proteins in this buffer system, the protein molecular weight can be roughly determined.
