Color pre dyed protein molecular weight standard: 1. This product contains 9 highly purified and pre stained recombinant proteins ranging from 25kDa to 300kDa. 2. Suitable as a protein molecular weight standard for SDS-PAGE and Western blotting. 3. This color pre stained protein molecular weight standard has been prepared in 1 × SDS-PAGE buffer and should be used directly without boiling, diluting, or adding reducing agents. 4. According to the size of the sample well, this color pre stained protein molecular weight standard usually requires 10 microliters of sample per loading (10μl for 5 × 1.5mm gel wells is sufficient), and very clear protein bands can be observed during electrophoresis, electrophoresis, and membrane transfer. The pre stained protein molecular weight standard, calibrated with non pre stained protein molecular weight standards, has the following outstanding advantages: 1. The blue and green bands showed little change in apparent molecular weight on SDS-PAGE gel in Tris Glycine, Bis Tris, and Tris Acetate buffer systems. 2. The orange (red) color stripes are consistent with the changes in imported products. Usage method: 1. After thawing at room temperature, completely dissolve and gently mix well, do not boil. 2. Take 10µl of this product and test sample for polyacrylamide gel electrophoresis at the same time; It is recommended that laboratories with conditions determine the appropriate sample size through pre experiments based on their own experimental conditions and habits when using this product for the first time. This can save costs and obtain better experimental images. 3. Unused color pre dyed protein molecular weight standards should be stored under storage conditions and can be left at 4°C for 2 months. Matters needing attention: 1. Pay attention to the electrophoresis time, as low molecular weight proteins may swim out of the protein gel front. 2. When Western blotting, attention should be paid to the specificity of small molecules, especially for bands smaller than 3kDa, which are prone to pass through the membrane. Additionally, it is recommended not to add SDS to the transfer solution. If it is necessary for the experiment, the concentration of SDS should not exceed 0.02% -0.04%. 3. Pre stained proteins have different apparent molecular weights in different buffer systems. If non pre stained proteins are calibrated in advance in this buffer system, the protein molecular weight can be roughly determined.
