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TIM 3 Mouse mAb - Primary antibody, specific to TIM 3, Mouse IgG1

Azide Free
Carrier Free
ExactAb™
Validated
Lot by Lot

Features and benefits

Short Overview:
mAb (F38-2E2); Mouse anti Human TIM 3 Antibody; Flow; Unconjugated
Species reactivity(Reacts with): Human
Isotype: Mouse IgG1

Application:

Flow Cytometry

Item Number

Ab131262

Grouped product items
SKU	Size	Availability	Price
Ab131262-10μg	10μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$56.90
Ab131262-50μg	50μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$169.90
Ab131262-100μg	100μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$219.90
Ab131262-1mg	1mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$1,999.90

mAb (F38-2E2); Mouse anti Human TIM 3 Antibody; Flow; Unconjugated

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Accession#:Q8TDQ0 Gene ID:84868 HAVCR2 TIM 3

Basic Description

Product Name	TIM 3 Mouse mAb - Primary antibody, specific to TIM 3, Mouse IgG1
Synonyms	CD366; FLJ14428; HAVCR2; HAVcr-2; hepatitis A virus cellular receptor 2; kidney injury molecule-3; KIM-3; SPTCL; T cell immunoglobulin mucin 3; T cell immunoglobulin mucin-3; T-cell immunoglobulin and mucin domain-containing protein 3; TIM 3; TIM3 T-cell
Specifications & Purity	ExactAb™, Validated, Carrier Free, Azide Free, Lot by Lot
Host species	Mouse
Specificity	TIM 3
Immunogen	Human Tim-3 fusion protein
Positive Control	Flow: Human peripheral blood lymphocytes.
Conjugation	Unconjugated
Grade	Azide Free, Carrier Free, ExactAb™, Validated
Product Description	Mouse anti Human TIM 3 Antibody, Monoclonal (F38-2E2), could be used for Flow and so on. Application: Flow: 1.0 μg/100w cells Protein Function Cell surface receptor implicated in modulating innate and adaptive immune responses. Generally accepted to have an inhibiting function. Reports on stimulating functions suggest that the activity may be influenced by the cellular context and/or the respective ligand (PubMed:24825777). Regulates macrophage activation (PubMed:11823861). Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance (PubMed:14556005). In CD8+ cells attenuates TCR-induced signaling, specifically by blocking NF-kappaB and NFAT promoter activities resulting in the loss of IL-2 secretion. The function may implicate its association with LCK proposed to impair phosphorylation of TCR subunits, and/or LGALS9-dependent recruitment of PTPRC to the immunological synapse (PubMed:24337741, PubMed:26492563). In contrast, shown to activate TCR-induced signaling in T-cells probably implicating ZAP70, LCP2, LCK and FYN (By similarity). Expressed on Treg cells can inhibit Th17 cell responses (PubMed:24838857). Receptor for LGALS9 (PubMed:16286920, PubMed:24337741). Binding to LGALS9 is believed to result in suppression of T-cell responses; the resulting apoptosis of antigen-specific cells may implicate HAVCR2 phosphorylation and disruption of its association with BAG6. Binding to LGALS9 is proposed to be involved in innate immune response to intracellular pathogens. Expressed on Th1 cells interacts with LGALS9 expressed on Mycobacterium tuberculosis-infected macrophages to stimulate antibactericidal activity including IL-1 beta secretion and to restrict intracellular bacterial growth (By similarity). However, the function as receptor for LGALS9 has been challenged (PubMed:23555261). Also reported to enhance CD8+ T-cell responses to an acute infection such as by Listeria monocytogenes (By similarity). Receptor for phosphatidylserine (PtSer); PtSer-binding is calcium-dependent. May recognize PtSer on apoptotic cells leading to their phagocytosis. Mediates the engulfment of apoptotic cells by dendritic cells. Expressed on T-cells, promotes conjugation but not engulfment of apoptotic cells. Expressed on dendritic cells (DCs) positively regulates innate immune response and in synergy with Toll-like receptors promotes secretion of TNF-alpha. In tumor-imfiltrating DCs suppresses nucleic acid-mediated innate immune repsonse by interaction with HMGB1 and interfering with nucleic acid-sensing and trafficking of nucleid acids to endosomes (By similarity). Expressed on natural killer (NK) cells acts as a coreceptor to enhance IFN-gamma production in response to LGALS9 (PubMed:22323453). In contrast, shown to suppress NK cell-mediated cytotoxicity (PubMed:22383801). Negatively regulates NK cell function in LPS-induced endotoxic shock.

Product Properties

Isotype	Mouse IgG1
Light Chain Type	kappa
SDS-PAGE	27.7 kDa (Light Chain) & 51.1 kDa (Heavy Chain), under reducing conditions; 188.4 kDa, under non-reducing conditions.
Purification Method	Protein G purified
Form	Liquid
Concentration	Lot by Lot
Storage Temp	Store at 2-8°C
Shipped In	Wet ice
Stability And Storage	Shipped at 4°C. Store at 4°C long term (12 months). Upon delivery aliquot.

Images

TIM 3 Mouse mAb (Ab131262) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood monocytes labelling TIM 3 with TIM 3 Mouse mAb (Ab131262) conjugated with biotin (right panel) at 0.1 μg/mL for 1 hour at 4°C, compared with mouse IgG (Ab170221) conjugated with biotin - Isotype Control (left panel) and detected with SA-PE (rp156250).

TIM 3 Mouse mAb (Ab131262) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood monocytes labelling TIM 3 (red) with TIM 3 Mouse mAb (Ab131262) conjugated with biotin at 0.1 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156250). Blue - Isotype control, mouse IgG (Ab170221) conjugated with biotin. Black - Unlabelled control, cells without incubation with primary antibody.

Application

Dilution info

Flow

1μg/100w cells

Certificates

Certificate of Analysis(COA)

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2 results found

Lot Number	Certificate Type	Date	Item
ZJ23F1101542	Certificate of Analysis	Nov 02, 2023	Ab131262
ZJ23F1101541	Certificate of Analysis	Nov 02, 2023	Ab131262

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