Ingredient description: Bottle 1: Buffer solution (28 mL; pH 8.0) added with sodium azide (0.02% w/v) as a preservative, stable for more than 2 years at 4 ° C. Bottle 2: NADH, freeze-dried powder., Stable below -10 ° C for more than 2 years. Bottle 3: NADH peroxidase solution (1.1 mL), stable for more than 2 years below -10 ° C. Bottle 4: Suspension of sulfite oxidase (1.1 mL), stable for more than 2 years at 4 ° C. Bottle 5: Sodium sulfite standard powder (~5g), stable for>5 years; Store in a sealed container at room temperature. instructions: 1. Use the contents of the accompanying bottle 1. 2. Dissolve the contents of bottle 2 in 11 milliliters of distilled water. 3 & 4. Use the contents of bottles 3 and 4 that come with it. before When opening for the first time, shake the bottle to remove any enzymes that may have deposited on the rubber stopper. Subsequently, store the bottle vertically. Mix the contents of the bottle before use. 5. Weigh 1 gram of citric acid into a 1-liter volumetric flask, dilute to 1L with distilled water, and dissolve. Accurately add 590 milligrams of sodium sulfite, dissolved and directly used for laboratory analysis. This sulfite solution is equivalent to 300mg/L SO2 The sulfite in the solution is unstable and will lose about 2% of its sulfite content per hour, so the solution must be kept fresh on the day of use.
Analyte: Total Sulfite Assay Format: Spectrophotometer, Microplate, Auto-analyser Detection Method: Absorbance Wavelength (nm): 340 Signal Response: Decrease Linear Range: 1 to 50 µg of sulfite per assay Limit of Detection: 0.34 mg/L Total Assay Time: ~ 30 min Application examples: Wine, beer, fruit juices, soft drinks, jam, fruit and vegetables, pharmaceuticals and other materials.
