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transfection reagent - 5.0mg/ml solution, high purity
Basic Description Specifications & Purity 5.0mg/ml solution Storage Temp Store at 2-8°C Shipped In Wet ice Product Description A new generation of cationic polymer transfection reagent, It has the characteristics of high transfection efficiency and low cytotoxicity. Compared with other transfection reagents, this product was more effective ® It is stable and can not be eliminated by serum, which makes gene transfection easy and repeatable.It has been successfully used in gene transfection of many primary cultured cells and transformed cell lines. It is widely used in domestic laboratories.
Characteristic
The transfection efficiency was high, and high transfection efficiency could be obtained in cell culture medium with or without serum;
Low cytotoxicity, suitable for many primary culture cells / transformed cell lines transfection, transient / stable transfection, adherent / suspension cell transfection;
The transfection procedure is simple, and the transfection experiment can be completed in half an hour.
Transportation and storage
5.0 mg/ml solution, please store at 4 ℃, do not freeze.
Some cell lines were successfully transfected by this transfection reagent
cell line source Cell type HEK 293 Human Embryonic kidney HeLa Human Cervix carcinoma NIH 3T3 Mouse Embryo fibroblast BNL CL2 Mouse Embryonic kidney cells HepG2 Human Hepatocarcinoma COS7 Monkey SV40 liver transformed CHO Chinese hamster Ovary SHSY-5Y Human Neuroblastoma IMR 32 Human Neuroblastoma MRC5 Human Fetal lung epithelium MCF7 Human Breast adenocarcinoma K562 Human Chronic leukemia SKOV-3 Human Ovarian adenocarcinoma IGROV-1 Human Ovarian adenocarcinoma HUV-EC(primary cell) Huamn Umbilical vein endothelial cell C6 Rat Glioma C6 cell Primary cell Rat Neonatal Rat Cardiomyocyte SGC-7901 Human Gastric cancer Eca-109 Human Esophageal carcinoma SK-N-SH Human Neuroblastoma ZR-75-1 Human Breast cancer THP-1 Human Monocyte WEHI231 Mouse Immature B SK-HEP-1 Human Liver adenocarcinoma P815 Mouse Mastocytoma BGC-823 Human Gastric carcinoma CV-1 African green monkey Kidney SiHa Human Cervical carcinoma A549 Human Lung carcinoma MDA-MB-231 Human Breast adenocarcinoma T-47D Human Breast cancer U-937 Human Histiocytic lymphoma HuH-7 Human Hepatoma C929 Human Bbone marrow XG-1 Human Myeloma MC3T3-E1 Rat Osteogenic Hep-2 Human Larynx carcinoma squamou MDCK Dog Kidney Madin-Darby
BMSC Mouse Bone marrow stromal BEL-7402 Human Hhepatic carcinoma 2BS Human Diploid fibroblast U373 Human Glioblastoma astrocytoma
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