Basic Description

Synonyms	1185-53-1\|Tris hydrochloride\|Tris(hydroxymethyl)aminomethane hydrochloride\|2-Amino-2-(hydroxymethyl)propane-1,3-diol hydrochloride\|Tromethamine hydrochloride\|TRIS HCl\|Tris-HCl\|Trometamol hydrochloride\|2-Amino-2-(hydroxymethyl)-1,3-propanediol Hydrochlorid
Specifications & Purity	>99% (titration), pH range 7.0 - 9.0, suitable for FISH
Storage Temp	Room temperature
Shipped In	Normal
Product Description	Application The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode. Buffering agent in incubation mixtures. It has also been used as a component of lysis and TE (Tris-EDTA) buffer. Other Notes For life science research only. Not for use in diagnostic procedures.

Names and Identifiers

IUPAC Name	2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride
INCHI	InChI=1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H
InChi Key	QKNYBSVHEMOAJP-UHFFFAOYSA-N
Canonical SMILES	C(C(CO)(CO)N)O.Cl
Isomeric SMILES	C(C(CO)(CO)N)O.Cl
WGK Germany	1
PubChem CID	93573
Molecular Weight	157.6
Beilstein	3675235

Certificates

Certificate of Analysis(COA)

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1 results found

Lot Number	Certificate Type	Date	Item
J2318291	Certificate of Analysis	Sep 21, 2023	T431526

Chemical and Physical Properties

Sensitivity	moisture sensitive
Melt Point(°C)	150-152°C

Safety and Hazards(GHS)

Pictogram(s)	GHS07
Signal	Warning
Hazard Statements	H315:Causes skin irritation H319:Causes serious eye irritation H335:May cause respiratory irritation
Precautionary Statements	P261:Avoid breathing dust/fume/gas/mist/vapors/spray. P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do - continue rinsing. P280:Wear protective gloves/protective clothing/eye protection/face protection. P302+P352:IF ON SKIN: wash with plenty of water. P405:Store locked up. P501:Dispose of contents/container to ... P264:Wash hands [and …] thoroughly after handling. P271:Use only outdoors or in a well-ventilated area. P304+P340:IF INHALED: Remove person to fresh air and keep comfortable for breathing. P403+P233:Store in a well-ventilated place. Keep container tightly closed. P362+P364:Take off contaminated clothing and wash it before reuse.
WGK Germany	1

References

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8. Shanker Shyam Sundhar Panchapakesan,Sunny C Y Jeng,Peter J Unrau. (2018-04-05) Purification of RNA Mango Tagged Native RNA-protein Complexes from Cellular Extracts Using TO1-Desthiobiotin Fluorophore Ligand.. Bio-protocol, 8 ((7)): (e2799-e2799). [PMID:34286019]
9. Cristina Sánchez-González,Laura Formentini. (2021-08-26) An optimized protocol for coupling oxygen consumption rates with β-oxidation in isolated mitochondria from mouse soleus.. STAR protocols, 2 ((3)): (100735-100735). [PMID:34430910]
10. Sylwia Mazurek,Urszula Oleksiewicz,Patrycja Czerwińska,Joanna Wróblewska,Marta Klimczak,Maciej Wiznerowicz. (2021-08-28) Disruption of RING and PHD Domains of TRIM28 Evokes Differentiation in Human iPSCs.. Cells, 10 ((8)): [PMID:34440702]
11. Diéssica Padilha Dalenogare,Camila Ritter,Fernando Roberto Antunes Bellinaso,Sabrina Qader Kudsi,Gabriele Cheiran Pereira,Maria Fernanda Pessano Fialho,Débora Denardin Lückemeyer,Caren Tatiane de David Antoniazzi,Lorenzo Landini,Juliano Ferreira,Guilherme Vargas Bochi,Sara Marchesan Oliveira,Francesco De Logu,Romina Nassini,Pierangelo Geppetti,Gabriela Trevisan. (2021-08-29) Periorbital Nociception in a Progressive Multiple Sclerosis Mouse Model Is Dependent on TRPA1 Channel Activation.. Pharmaceuticals (Basel, Switzerland), 14 ((8)): [PMID:34451927]
12. Cynthia Vanesa Rivero,Santiago José Martínez,Paul Novick,Juan Agustín Cueto,Betiana Nebaí Salassa,María Cristina Vanrell,Xiaomo Li,Carlos Alberto Labriola,Luis Mariano Polo,David M Engman,Joachim Clos,Patricia Silvia Romano. (2021-09-04) Repurposing Carvedilol as a Novel Inhibitor of the Trypanosoma cruzi Autophagy Flux That Affects Parasite Replication and Survival.. Frontiers in cellular and infection microbiology, 11 (657257-657257). [PMID:34476220]
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Tris hydrochloride - >99% (titration), pH range 7.0 - 9.0, suitable for FISH, high purity , CAS No.1185-53-1