TRIS hydrochloride - BioReagent Plus, ≥99%, high purity , CAS No.1185-53-1

Item Number

T431531

• Synonyms: 1185-53-1|Tris hydrochloride|Tris(hydroxymethyl)aminomethane hydrochloride|2-Amino-2-(hydroxymethyl)propane-1,3-diol hydrochloride|Tromethamine hydrochloride|TRIS HCl|Tris-HCl|Trometamol hydrochloride|2-Amino-2-(hydroxymethyl)-1,3-propanediol Hydrochlorid
• Specifications & Purity: BioReagent Plus, ≥99%
• Grade: BioReagent Plus

Product Description

Application

The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode. Tris Hydrochloride is a commonly used biological buffer which is also referred to as Trizma HCl. Tris as a buffer has very broad usage in biological systems such as diagnostics, downstream purification and cell culture. The pKa of Tris is 7.77 which corresponds closely to a physiological pH of 7.36. Tris HCl s used in biopharmaceutical buffer formulations (both upstream and downstream), electrophoresis separations and diagnostic reagent formulations. Tris HCl is especially useful in downstream purification processes.