Basic Description

Synonyms	TRYPAN BLUE \| Direct Blue 14 \| 72-57-1 \| Niagara Blue \| C.I. Direct Blue 14 \| VisionBlue \| Benzamine Blue \| Dianil blue \| Diamine Blue \| Benzo Blue \| Congo Blue \| Naphthylamine Blue \| Diphenyl Blue \| C.I. 23850 \| Niagara Blue 3B \| trypan-blue \| Diamineblue \| Dianilblau \| Pyrotropblau \| Parkib
Specifications & Purity	0.4% solv., for tissue culture
Storage Temp	Desiccated
Shipped In	Normal
Product Description	Trypan Blue is an azo based, hydrophilic, tetrasulfonated blue acid dye widely utilized for assessing cell viability. As an easy method to determining the viability of cells, Trypan Blue will stain dead cells with permeable membranes blue, while the dye is excluded by most living cells and their intact membranes thereby allowing visual determination of living versus dead cells. Although Trypan Blue is predominately used for assessing cell viability of cultured cells, other applications for this dye have been reported. In rat abdominal organ slices Trypan Blue has been utilized to determine cell viability as well as islet cell cluster cell viability from caprine pancreatic samples. A viability dye for quantifying live versus dead cells

Names and Identifiers

IUPAC Name	tetrasodium;5-amino-3-[[4-[4-[(8-amino-1-hydroxy-3,6-disulfonatonaphthalen-2-yl)diazenyl]-3-methylphenyl]-2-methylphenyl]diazenyl]-4-hydroxynaphthalene-2,7-disulfonate
INCHI	InChI=1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4
InChi Key	GLNADSQYFUSGOU-UHFFFAOYSA-J
Canonical SMILES	CC1=C(C=CC(=C1)C2=CC(=C(C=C2)N=NC3=C(C4=C(C=C(C=C4C=C3S(=O)(=O)[O-])S(=O)(=O)[O-])N)O)C)N=NC5=C(C6=C(C=C(C=C6C=C5S(=O)(=O)[O-])S(=O)(=O)[O-])N)O.[Na+].[Na+].[Na+].[Na+]
Isomeric SMILES	CC1=C(C=CC(=C1)C2=CC(=C(C=C2)N=NC3=C(C4=C(C=C(C=C4C=C3S(=O)(=O)[O-])S(=O)(=O)[O-])N)O)C)N=NC5=C(C6=C(C=C(C=C6C=C5S(=O)(=O)[O-])S(=O)(=O)[O-])N)O.[Na+].[Na+].[Na+].[Na+]
WGK Germany	3
RTECS	QJ6475000
PubChem CID	6296
Molecular Weight	960.81
Beilstein	4360496

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Chemical and Physical Properties

Solubility	Soluble in water (10 mg/ml at 25 °C), ethanol (0.6 mg/ml), and EGME (20 mg/ml).c
Melt Point(°C)	>300°C

Safety and Hazards(GHS)

Pictogram(s)	GHS08
Signal	Danger
Hazard Statements	H350:May cause cancer
Precautionary Statements	P280:Wear protective gloves/protective clothing/eye protection/face protection. P405:Store locked up. P501:Dispose of contents/container to ... P203:Obtain, read and follow all safety instructions before use. P318:if exposed or concerned, get medical advice.
WGK Germany	3
RTECS	QJ6475000
Merck Index	9792

Citations of This Product

1. Kankan Chen, Jiawei Xu, Jingjing Song, Zhonggang Liu, Zheng Guo. (2023) A facile ratiometric electrochemical sensing platform with in-situ electropolymerized thionine on effective sensing of 5-hydroxytryptamine. ELECTROANALYSIS, 36 (3): (e202300324). [PMID:15522303]
2. Guangyong Zeng, Hu Zheng, Kun Zhou, Hao Shi, Size Zheng, Hui Ma, Peng Wang, Shengyan Pu. (2023) Preparation, Characterization and Application of Novel Photocatalytic Two-Dimensional Material Membrane: A Reform of Comprehensive Experimental Teaching. Sustainability, 15 (14): (11342). [PMID:]
3. He Zhang, Wenmei Luo, Yuping Du, Hao Shi, Guangyong Zeng, Xinxing Yan, Xiaoke Li. (2023) The g-C3N4 decorated carbon aerogel with integrated solar steam generation and photocatalysis for effective desalination and water purification. DESALINATION, 564 (116821). [PMID:]
4. Ling Jiang, Huan Huang, Xingyu Shi, Jian Wu, Juexian Ye, Qian Xu, Shaobin Fang, Chuanbin Wu, Rui Luo, Chao Lu, Daojun Liu. (2023) Biocontrol Microneedle Patch: A Promising Agent for Protecting Citrus Fruits from Postharvest Infection. Pharmaceutics, 15 (4): (1219). [PMID:37111704]
5. Xin-Lei Cheng, Ting-Rui Fu, Dan-Feng Zhang, Jian-Hua Xiong, Wu-Ying Yang, Juan Du. (2023) Biomass-assisted fabrication of rGO-AuNPs as surface-enhanced Raman scattering substrates for in-situ monitoring methylene blue degradation. ANALYTICAL BIOCHEMISTRY, 667 (115087). [PMID:36858251]
6. Yujiao Tu, Ze Liu, Lei Jiang, Yingying Xiang, Fei Song, Lifen Meng, Xiuling Ji, Lin Yuan. (2023) Green ratiometric fluorescent dual-mode nanosensor for highly selective and sensitive detection of new coccine in food. DYES AND PIGMENTS, 210 (111024). [PMID:]
7. Wang Yongtao, Wang Nana, Yang Yingjun, Chen Yazhou, Zhang Zhengguo. (2022) Cellular nanomechanics derived from pattern-dependent focal adhesion and cytoskeleton to balance gene transfection of malignant osteosarcoma. JOURNAL OF NANOBIOTECHNOLOGY, 20 (1): (1-13). [PMID:36424661]
8. Lin Yuan, Lei Jiang, Ze Liu, Yingying Xiang, Fei Song, Yujiao Tu. (2023) Selective and sensitive simultaneous detection of tartrazine and Hg2+ based on N-doped yellow-green fluorescent carbon dots. DYES AND PIGMENTS, 209 (110893). [PMID:]
9. Jin Wen, Xiaoke Li, He Zhang, Size Zheng, Caini Yi, Liu Yang, Jinwen Shi. (2023) Architecting Janus hydrogel evaporator with polydopamine-TiO2 photocatalyst for high-efficient solar desalination and purification. SEPARATION AND PURIFICATION TECHNOLOGY, 304 (122403). [PMID:]
10. Yingying Xiang, Yujiao Tu, Lei Jiang, Lin Yuan, Ze Liu, Qiang Xie, Xuequan Xiong, Fei Song. (2022) Orange fluorescent dual-mode nanoprobe for selective and sensitive detection of amaranth based on S,N-Doped carbon dots. DYES AND PIGMENTS, 205 (110533). [PMID:]
11. Siyuan Huang, Qi Su, Xiaoqiang Hou, Kuankuan Han, Shufang Ma, Bingshe Xu, Yingjun Yang. (2022) Influence of Colonies’ Morphological Cues on Cellular Uptake Capacity of Nanoparticles. Frontiers in Bioengineering and Biotechnology, 10 (922159). [PMID:35711638]
12. Xiaojie Cheng, Jiahui Liao, Yue Xue, Qingquan Lin, Zhaomei Yang, Guilong Yan, Guangyong Zeng, Arijit Sengupta. (2022) Ultrahigh-flux and self-cleaning composite membrane based on BiOCl-PPy modified MXene nanosheets for contaminants removal from wastewater. JOURNAL OF MEMBRANE SCIENCE, 644 (120188). [PMID:]
13. Song Zhu, Fei Luo, Bin Zhu, Fei Ling, Er-Long Wang, Tian-Qiang Liu, Gao-Xue Wang. (2021) A Nanobody-Mediated Virus-Targeting Drug Delivery Platform for the Central Nervous System Viral Disease Therapy. Microbiology Spectrum, 9 (3): [PMID:34817277]
14. Hong Cheng,Gui-Ling Fan,Jing-Hao Fan,Ping Yuan,Fu-An Deng,Xiao-Zhong Qiu,Xi-Yong Yu,Shi-Ying Li. (2019-09-22) Epigenetics-inspired photosensitizer modification for plasma membrane-targeted photodynamic tumor therapy.. Biomaterials, 224 (119497-119497). [PMID:31541935]

References

1. Kankan Chen, Jiawei Xu, Jingjing Song, Zhonggang Liu, Zheng Guo. (2023) A facile ratiometric electrochemical sensing platform with in-situ electropolymerized thionine on effective sensing of 5-hydroxytryptamine. ELECTROANALYSIS, 36 (3): (e202300324). [PMID:15522303]
2. Guangyong Zeng, Hu Zheng, Kun Zhou, Hao Shi, Size Zheng, Hui Ma, Peng Wang, Shengyan Pu. (2023) Preparation, Characterization and Application of Novel Photocatalytic Two-Dimensional Material Membrane: A Reform of Comprehensive Experimental Teaching. Sustainability, 15 (14): (11342). [PMID:]
3. He Zhang, Wenmei Luo, Yuping Du, Hao Shi, Guangyong Zeng, Xinxing Yan, Xiaoke Li. (2023) The g-C3N4 decorated carbon aerogel with integrated solar steam generation and photocatalysis for effective desalination and water purification. DESALINATION, 564 (116821). [PMID:]
4. Ling Jiang, Huan Huang, Xingyu Shi, Jian Wu, Juexian Ye, Qian Xu, Shaobin Fang, Chuanbin Wu, Rui Luo, Chao Lu, Daojun Liu. (2023) Biocontrol Microneedle Patch: A Promising Agent for Protecting Citrus Fruits from Postharvest Infection. Pharmaceutics, 15 (4): (1219). [PMID:37111704]
5. Xin-Lei Cheng, Ting-Rui Fu, Dan-Feng Zhang, Jian-Hua Xiong, Wu-Ying Yang, Juan Du. (2023) Biomass-assisted fabrication of rGO-AuNPs as surface-enhanced Raman scattering substrates for in-situ monitoring methylene blue degradation. ANALYTICAL BIOCHEMISTRY, 667 (115087). [PMID:36858251]
6. Yujiao Tu, Ze Liu, Lei Jiang, Yingying Xiang, Fei Song, Lifen Meng, Xiuling Ji, Lin Yuan. (2023) Green ratiometric fluorescent dual-mode nanosensor for highly selective and sensitive detection of new coccine in food. DYES AND PIGMENTS, 210 (111024). [PMID:]
7. Wang Yongtao, Wang Nana, Yang Yingjun, Chen Yazhou, Zhang Zhengguo. (2022) Cellular nanomechanics derived from pattern-dependent focal adhesion and cytoskeleton to balance gene transfection of malignant osteosarcoma. JOURNAL OF NANOBIOTECHNOLOGY, 20 (1): (1-13). [PMID:36424661]
8. Lin Yuan, Lei Jiang, Ze Liu, Yingying Xiang, Fei Song, Yujiao Tu. (2023) Selective and sensitive simultaneous detection of tartrazine and Hg2+ based on N-doped yellow-green fluorescent carbon dots. DYES AND PIGMENTS, 209 (110893). [PMID:]
9. Jin Wen, Xiaoke Li, He Zhang, Size Zheng, Caini Yi, Liu Yang, Jinwen Shi. (2023) Architecting Janus hydrogel evaporator with polydopamine-TiO2 photocatalyst for high-efficient solar desalination and purification. SEPARATION AND PURIFICATION TECHNOLOGY, 304 (122403). [PMID:]
10. Yingying Xiang, Yujiao Tu, Lei Jiang, Lin Yuan, Ze Liu, Qiang Xie, Xuequan Xiong, Fei Song. (2022) Orange fluorescent dual-mode nanoprobe for selective and sensitive detection of amaranth based on S,N-Doped carbon dots. DYES AND PIGMENTS, 205 (110533). [PMID:]
11. Siyuan Huang, Qi Su, Xiaoqiang Hou, Kuankuan Han, Shufang Ma, Bingshe Xu, Yingjun Yang. (2022) Influence of Colonies’ Morphological Cues on Cellular Uptake Capacity of Nanoparticles. Frontiers in Bioengineering and Biotechnology, 10 (922159). [PMID:35711638]
12. Xiaojie Cheng, Jiahui Liao, Yue Xue, Qingquan Lin, Zhaomei Yang, Guilong Yan, Guangyong Zeng, Arijit Sengupta. (2022) Ultrahigh-flux and self-cleaning composite membrane based on BiOCl-PPy modified MXene nanosheets for contaminants removal from wastewater. JOURNAL OF MEMBRANE SCIENCE, 644 (120188). [PMID:]
13. Song Zhu, Fei Luo, Bin Zhu, Fei Ling, Er-Long Wang, Tian-Qiang Liu, Gao-Xue Wang. (2021) A Nanobody-Mediated Virus-Targeting Drug Delivery Platform for the Central Nervous System Viral Disease Therapy. Microbiology Spectrum, 9 (3): [PMID:34817277]
14. Hong Cheng,Gui-Ling Fan,Jing-Hao Fan,Ping Yuan,Fu-An Deng,Xiao-Zhong Qiu,Xi-Yong Yu,Shi-Ying Li. (2019-09-22) Epigenetics-inspired photosensitizer modification for plasma membrane-targeted photodynamic tumor therapy.. Biomaterials, 224 (119497-119497). [PMID:31541935]

Solution Calculators

Molarity Calculator

Determine the necessary mass, volume, or concentration for preparing a solution.

Dilution Calculator

Determine the dilution needed to prepare a stock solution.

Trypan Blue - 0.4% solv., for tissue culture, high purity , CAS No.72-57-1

Basic Description

Names and Identifiers

Certificates

Certificate of Analysis(COA)

Chemical and Physical Properties

Safety and Hazards(GHS)

Related Documents

Citations of This Product

References

Solution Calculators

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

SKU	Size	Availability	Price	Qty
T108744-25ml	25ml	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$24.90
T108744-100ml	100ml	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$55.90