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Trypan Blue - Biological stain, high purity , CAS No.72-57-1

14 Citations
Item Number
T108745
Grouped product items
SKUSizeAvailabilityPrice Qty
T108745-5g
5g
In stock
$34.90
T108745-25g
25g
In stock
$120.90
T108745-100g
100g
In stock
$323.90
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Basic Description

SynonymsC.I. 23850 | Centraline Blue 3B | Bleue diretto 3B | Modr Prima 14 [Czech] | Sodium ditolyl-diazobis-8-amino-1-naphthol-3,6-disulfonate | Benzanil Blue R | Congoblau 3B | Dianilblau H3G | TRYPAN BLUE [MART.] | Diaminblau 3B | NCGC00167554-01 | TRYPAN BLUE
Specifications & PurityBiological stain
Storage TempDesiccated
Shipped InNormal
GradeBiological stain
Product Description

Product Introduction

Trypan Blue is an azo based, hydrophilic, tetrasulfonated blue acid dye widely utilized for assessing cell viability. As an easy method to determining the viability of cells, Trypan Blue will stain dead cells with permeable membranes blue, while the dye is excluded by most living cells and their intact membranes thereby allowing visual determination of living versus dead cells. Although Trypan Blue is predominately used for assessing cell viability of cultured cells, other applications for this dye have been reported. In rat abdominal organ slices Trypan Blue has been utilized to determine cell viability as well as islet cell cluster cell viability from caprine pancreatic samples.
A viability dye for quantifying live versus dead cells


The trypan blue dye is an azo dye, derived from toluidine. It is soluble in water and has a molecular weight of 961 g/mol.


Application

Trypan blue has been used:

in cell viability assay

to determine the number of living and necrotic cells by staining with 0.2% trypan blue solution.

to stain plant tissue in order to to visualize cell death

Names and Identifiers

IUPAC Name tetrasodium;5-amino-3-[[4-[4-[(8-amino-1-hydroxy-3,6-disulfonatonaphthalen-2-yl)diazenyl]-3-methylphenyl]-2-methylphenyl]diazenyl]-4-hydroxynaphthalene-2,7-disulfonate
INCHI InChI=1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4
InChi Key GLNADSQYFUSGOU-UHFFFAOYSA-J
Canonical SMILES CC1=C(C=CC(=C1)C2=CC(=C(C=C2)N=NC3=C(C4=C(C=C(C=C4C=C3S(=O)(=O)[O-])S(=O)(=O)[O-])N)O)C)N=NC5=C(C6=C(C=C(C=C6C=C5S(=O)(=O)[O-])S(=O)(=O)[O-])N)O.[Na+].[Na+].[Na+].[Na+]
Isomeric SMILES CC1=C(C=CC(=C1)C2=CC(=C(C=C2)N=NC3=C(C4=C(C=C(C=C4C=C3S(=O)(=O)[O-])S(=O)(=O)[O-])N)O)C)N=NC5=C(C6=C(C=C(C=C6C=C5S(=O)(=O)[O-])S(=O)(=O)[O-])N)O.[Na+].[Na+].[Na+].[Na+]
WGK Germany 3
RTECS QJ6475000
PubChem CID 6296
Molecular Weight 960.81
Beilstein 4360496

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

7 results found

Lot NumberCertificate TypeDateItem
C2004044Certificate of AnalysisDec 20, 2023 T108745
E2317899Certificate of AnalysisJan 05, 2023 T108745
E2317900Certificate of AnalysisJan 05, 2023 T108745
E2317904Certificate of AnalysisJan 05, 2023 T108745
E2317906Certificate of AnalysisJan 05, 2023 T108745
E2317907Certificate of AnalysisJan 05, 2023 T108745
E2317911Certificate of AnalysisJan 05, 2023 T108745

Chemical and Physical Properties

SolubilitySoluble in water (10 mg/ml at 25 °C), ethanol (0.6 mg/ml), and EGME (20 mg/ml).
Melt Point(°C)>300°C

Safety and Hazards(GHS)

Pictogram(s) GHS08
Signal Danger
Hazard Statements

H350:May cause cancer

Precautionary Statements

P280:Wear protective gloves/protective clothing/eye protection/face protection.

P405:Store locked up.

P501:Dispose of contents/container to ...

P203:Obtain, read and follow all safety instructions before use.

P318:if exposed or concerned, get medical advice.

WGK Germany 3
RTECS QJ6475000
Merck Index 9792

Related Documents

 SDS

 Specification Sheet

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Citations of This Product

1. Kankan Chen, Jiawei Xu, Jingjing Song, Zhonggang Liu, Zheng Guo.  (2023)  A facile ratiometric electrochemical sensing platform with in-situ electropolymerized thionine on effective sensing of 5-hydroxytryptamine.  ELECTROANALYSIS,  36  (3): (e202300324).  [PMID:15522303]
2. Guangyong Zeng, Hu Zheng, Kun Zhou, Hao Shi, Size Zheng, Hui Ma, Peng Wang, Shengyan Pu.  (2023)  Preparation, Characterization and Application of Novel Photocatalytic Two-Dimensional Material Membrane: A Reform of Comprehensive Experimental Teaching.  Sustainability,  15  (14): (11342).  [PMID:]
3. He Zhang, Wenmei Luo, Yuping Du, Hao Shi, Guangyong Zeng, Xinxing Yan, Xiaoke Li.  (2023)  The g-C3N4 decorated carbon aerogel with integrated solar steam generation and photocatalysis for effective desalination and water purification.  DESALINATION,  564  (116821).  [PMID:]
4. Ling Jiang, Huan Huang, Xingyu Shi, Jian Wu, Juexian Ye, Qian Xu, Shaobin Fang, Chuanbin Wu, Rui Luo, Chao Lu, Daojun Liu.  (2023)  Biocontrol Microneedle Patch: A Promising Agent for Protecting Citrus Fruits from Postharvest Infection.  Pharmaceutics,  15  (4): (1219).  [PMID:37111704]
5. Xin-Lei Cheng, Ting-Rui Fu, Dan-Feng Zhang, Jian-Hua Xiong, Wu-Ying Yang, Juan Du.  (2023)  Biomass-assisted fabrication of rGO-AuNPs as surface-enhanced Raman scattering substrates for in-situ monitoring methylene blue degradation.  ANALYTICAL BIOCHEMISTRY,  667  (115087).  [PMID:36858251]
6. Yujiao Tu, Ze Liu, Lei Jiang, Yingying Xiang, Fei Song, Lifen Meng, Xiuling Ji, Lin Yuan.  (2023)  Green ratiometric fluorescent dual-mode nanosensor for highly selective and sensitive detection of new coccine in food.  DYES AND PIGMENTS,  210  (111024).  [PMID:]
7. Wang Yongtao, Wang Nana, Yang Yingjun, Chen Yazhou, Zhang Zhengguo.  (2022)  Cellular nanomechanics derived from pattern-dependent focal adhesion and cytoskeleton to balance gene transfection of malignant osteosarcoma.  JOURNAL OF NANOBIOTECHNOLOGY,  20  (1): (1-13).  [PMID:36424661]
8. Lin Yuan, Lei Jiang, Ze Liu, Yingying Xiang, Fei Song, Yujiao Tu.  (2023)  Selective and sensitive simultaneous detection of tartrazine and Hg2+ based on N-doped yellow-green fluorescent carbon dots.  DYES AND PIGMENTS,  209  (110893).  [PMID:]
9. Jin Wen, Xiaoke Li, He Zhang, Size Zheng, Caini Yi, Liu Yang, Jinwen Shi.  (2023)  Architecting Janus hydrogel evaporator with polydopamine-TiO2 photocatalyst for high-efficient solar desalination and purification.  SEPARATION AND PURIFICATION TECHNOLOGY,  304  (122403).  [PMID:]
10. Yingying Xiang, Yujiao Tu, Lei Jiang, Lin Yuan, Ze Liu, Qiang Xie, Xuequan Xiong, Fei Song.  (2022)  Orange fluorescent dual-mode nanoprobe for selective and sensitive detection of amaranth based on S,N-Doped carbon dots.  DYES AND PIGMENTS,  205  (110533).  [PMID:]
11. Siyuan Huang, Qi Su, Xiaoqiang Hou, Kuankuan Han, Shufang Ma, Bingshe Xu, Yingjun Yang.  (2022)  Influence of Colonies’ Morphological Cues on Cellular Uptake Capacity of Nanoparticles.  Frontiers in Bioengineering and Biotechnology,  10  (922159).  [PMID:35711638]
12. Xiaojie Cheng, Jiahui Liao, Yue Xue, Qingquan Lin, Zhaomei Yang, Guilong Yan, Guangyong Zeng, Arijit Sengupta.  (2022)  Ultrahigh-flux and self-cleaning composite membrane based on BiOCl-PPy modified MXene nanosheets for contaminants removal from wastewater.  JOURNAL OF MEMBRANE SCIENCE,  644  (120188).  [PMID:]
13. Song Zhu, Fei Luo, Bin Zhu, Fei Ling, Er-Long Wang, Tian-Qiang Liu, Gao-Xue Wang.  (2021)  A Nanobody-Mediated Virus-Targeting Drug Delivery Platform for the Central Nervous System Viral Disease Therapy.  Microbiology Spectrum,  9  (3):   [PMID:34817277]
14. Hong Cheng,Gui-Ling Fan,Jing-Hao Fan,Ping Yuan,Fu-An Deng,Xiao-Zhong Qiu,Xi-Yong Yu,Shi-Ying Li.  (2019-09-22)  Epigenetics-inspired photosensitizer modification for plasma membrane-targeted photodynamic tumor therapy..  Biomaterials,  224  (119497-119497).  [PMID:31541935]

References

1. Kankan Chen, Jiawei Xu, Jingjing Song, Zhonggang Liu, Zheng Guo.  (2023)  A facile ratiometric electrochemical sensing platform with in-situ electropolymerized thionine on effective sensing of 5-hydroxytryptamine.  ELECTROANALYSIS,  36  (3): (e202300324).  [PMID:15522303]
2. Guangyong Zeng, Hu Zheng, Kun Zhou, Hao Shi, Size Zheng, Hui Ma, Peng Wang, Shengyan Pu.  (2023)  Preparation, Characterization and Application of Novel Photocatalytic Two-Dimensional Material Membrane: A Reform of Comprehensive Experimental Teaching.  Sustainability,  15  (14): (11342).  [PMID:]
3. He Zhang, Wenmei Luo, Yuping Du, Hao Shi, Guangyong Zeng, Xinxing Yan, Xiaoke Li.  (2023)  The g-C3N4 decorated carbon aerogel with integrated solar steam generation and photocatalysis for effective desalination and water purification.  DESALINATION,  564  (116821).  [PMID:]
4. Ling Jiang, Huan Huang, Xingyu Shi, Jian Wu, Juexian Ye, Qian Xu, Shaobin Fang, Chuanbin Wu, Rui Luo, Chao Lu, Daojun Liu.  (2023)  Biocontrol Microneedle Patch: A Promising Agent for Protecting Citrus Fruits from Postharvest Infection.  Pharmaceutics,  15  (4): (1219).  [PMID:37111704]
5. Xin-Lei Cheng, Ting-Rui Fu, Dan-Feng Zhang, Jian-Hua Xiong, Wu-Ying Yang, Juan Du.  (2023)  Biomass-assisted fabrication of rGO-AuNPs as surface-enhanced Raman scattering substrates for in-situ monitoring methylene blue degradation.  ANALYTICAL BIOCHEMISTRY,  667  (115087).  [PMID:36858251]
6. Yujiao Tu, Ze Liu, Lei Jiang, Yingying Xiang, Fei Song, Lifen Meng, Xiuling Ji, Lin Yuan.  (2023)  Green ratiometric fluorescent dual-mode nanosensor for highly selective and sensitive detection of new coccine in food.  DYES AND PIGMENTS,  210  (111024).  [PMID:]
7. Wang Yongtao, Wang Nana, Yang Yingjun, Chen Yazhou, Zhang Zhengguo.  (2022)  Cellular nanomechanics derived from pattern-dependent focal adhesion and cytoskeleton to balance gene transfection of malignant osteosarcoma.  JOURNAL OF NANOBIOTECHNOLOGY,  20  (1): (1-13).  [PMID:36424661]
8. Lin Yuan, Lei Jiang, Ze Liu, Yingying Xiang, Fei Song, Yujiao Tu.  (2023)  Selective and sensitive simultaneous detection of tartrazine and Hg2+ based on N-doped yellow-green fluorescent carbon dots.  DYES AND PIGMENTS,  209  (110893).  [PMID:]
9. Jin Wen, Xiaoke Li, He Zhang, Size Zheng, Caini Yi, Liu Yang, Jinwen Shi.  (2023)  Architecting Janus hydrogel evaporator with polydopamine-TiO2 photocatalyst for high-efficient solar desalination and purification.  SEPARATION AND PURIFICATION TECHNOLOGY,  304  (122403).  [PMID:]
10. Yingying Xiang, Yujiao Tu, Lei Jiang, Lin Yuan, Ze Liu, Qiang Xie, Xuequan Xiong, Fei Song.  (2022)  Orange fluorescent dual-mode nanoprobe for selective and sensitive detection of amaranth based on S,N-Doped carbon dots.  DYES AND PIGMENTS,  205  (110533).  [PMID:]
11. Siyuan Huang, Qi Su, Xiaoqiang Hou, Kuankuan Han, Shufang Ma, Bingshe Xu, Yingjun Yang.  (2022)  Influence of Colonies’ Morphological Cues on Cellular Uptake Capacity of Nanoparticles.  Frontiers in Bioengineering and Biotechnology,  10  (922159).  [PMID:35711638]
12. Xiaojie Cheng, Jiahui Liao, Yue Xue, Qingquan Lin, Zhaomei Yang, Guilong Yan, Guangyong Zeng, Arijit Sengupta.  (2022)  Ultrahigh-flux and self-cleaning composite membrane based on BiOCl-PPy modified MXene nanosheets for contaminants removal from wastewater.  JOURNAL OF MEMBRANE SCIENCE,  644  (120188).  [PMID:]
13. Song Zhu, Fei Luo, Bin Zhu, Fei Ling, Er-Long Wang, Tian-Qiang Liu, Gao-Xue Wang.  (2021)  A Nanobody-Mediated Virus-Targeting Drug Delivery Platform for the Central Nervous System Viral Disease Therapy.  Microbiology Spectrum,  9  (3):   [PMID:34817277]
14. Hong Cheng,Gui-Ling Fan,Jing-Hao Fan,Ping Yuan,Fu-An Deng,Xiao-Zhong Qiu,Xi-Yong Yu,Shi-Ying Li.  (2019-09-22)  Epigenetics-inspired photosensitizer modification for plasma membrane-targeted photodynamic tumor therapy..  Biomaterials,  224  (119497-119497).  [PMID:31541935]

Solution Calculators

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