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Basic Description
| Stability And Storage | Store at 4℃ for up to 1 year, or at -20℃ for long term storage. | |||||||||||||||||||||||||||||
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| Storage Temp | Store at -20°C | |||||||||||||||||||||||||||||
| Shipped In | Ice chest + Ice pads | |||||||||||||||||||||||||||||
| Product Description | Trypsin-EDTA Solution(0.05% Trypsin) Aladdin's Trypsin-EDTA Solution (0.05% Trypsin) contains 0.05% trypsin and 0.02% EDTA, with a pH of 7.2-7.8. It is sterile and generally used for the digestion of cultured cells or tissues. This product is ready to use and works with high efficiency. Most adherent cells can be digested in about approximately 1 minute at room temperature. Trypsin-EDTA Solution(0.25% Trypsin;with phenol red) Aladdin's Trypsin-EDTA Solution with Phenol Red (0.25% Trypsin) contains 0.25% trypsin, 0.02% EDTA and phenol red, with a pH of 7.2-7.8. It is sterile and generally used for the digestion of cultured cells or tissues. This product is ready to use and works with high efficiency. Most adherent cells can be digested in about 1 minute at room temperature. Please see Figure 1 for the performance of this product in digesting different cell lines. Digestion result can be examined by a microscope. Stop cell digestion when a slight morphological change occurs and there is no need to wait until cells appear rounded. Figure 1. Different cell lines digested with the Trypsin-EDTA Solution with Phenol Red (Beytome, #C0203) for different duration period, as indicated in the figure. Cells were treated with the Trypsin-EDTA Solution with Phenol Red equilibrated to room temperature, and examined after 1-2 minutes by a microscope. It can be seen that the treated cells demonstrate contracted edges at the beginning and gradually dissociate to single cells in the end. This figure is for reference only. Experimental results may vary due to different cell lines and different experimental conditions. Trypsin-EDTA Solution(0.05% Trypsin;with phenol red) Aladdin's Trypsin-EDTA Solution with Phenol Red(0.05% Trypsin) contains 0.05% trypsin, 0.02% EDTA and phenol red, with a pH of 7.2-7.8. It is sterile and generally used for the digestion of cultured cells or tissues. Trypsin-EDTA Solution(0.25% Trypsin;without EDTA) This product is ready to use and works with high efficiency. Most adherent cells can be digested in approximately 1 minute at room temperature Aladdin's Trypsin Solution without EDTA (0.25% Trypsin) contains 0.25% trypsin and no EDTA, with a pH of 7.2-7.8. It is sterile and generally used for the digestion of cultured cells or tissues. This product is ready to use and works with high efficiency. Most adherent cells can be digested in approximately 1 minute at room temperature. Trypsin-EDTA Solution(0.25% Trypsin;withphenol red;without EDTA) Aladdin's Trypsin Solution without EDTA contains phenol red and 0.25% trypsin, with a pH of 7.4±0.1. It is sterile and generally used for the digestion of cultured cells or tissues. This product is ready to use and works with high efficiency. Most adherent cells can be digested in approximately 1 minute at room temperature.
Use aseptic techniques while handling the Trypsin-EDTA Solution to avoid microorganism contamination. Over digestion of cells should be avoided to prevent cells from being damaged and killed. It is recommended to take an appropriate amount of this product and equilibrate to room temperature or 37℃ before use if necessary. Do not equilibrate the whole bottle of this product to avoid the reduction of enzyme activity. This product is for R&D only. Not for drug, household, or other uses. For your safety and health, please wear lab coat and disposable gloves during the operation. |
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