Trypsin from bovine pancreas(Modified,Sequencing Grade) - ≥150 units/mg protein(at least 8,625 BAEE/2875 USP/NF units/mg protein), high purity , CAS No.9002-07-7

Item Number

T128769

• Synonyms: Peptidyl peptide hydrolase
• Specifications & Purity: ≥150 units/mg protein(at least 8,625 BAEE/2875 USP/NF units/mg protein)
• Biochemical and Physiological Mechanisms: Trypsin cleaves peptide chains, amides and esters mainly at the carboxyl side of the amino acids lysine or arginine. Trypsin originates in the pancreas in the form of the zymogen trypsinogen.
• Storage Temp: Store at -20°C
• Shipped In: Dry ice
• Grade: EnzymoPure™
• Note: Note: 1mg trypsin ≥180 TAME units, 10,350 BAEE units, 3,450 USP/NF units.

Product Description

Specificity:
Trypsin cleaves peptides on the C-terminal side of lysine and arginine amino acid residues. If a proline residue is on the carboxyl side of the cleavage site, the cleavage will not occur. If an acidic residue is on either side of the cleavage site, the rate of hydrolysis has been shown to be slower.
Protein Accession Number: P00760
CATH Classification (v. 3.2.0): 
Class: Mainly Beta
Architecture: Beta Barrel
Topology: Thrombin, subunit H
Molecular Weight: 23.3 kDa (Theoretical)
Optimal pH: 7.5-8.5 (Koutsopoulos et al. 2007)
Isoelectric Point:
Trypsinogen: pH 9.3 (Walsh and Neurath 1964)
Trypsin: pH 10.5 (Cunningham 1954).
Extinction Coefficient: Trypsinogen: 45,250 cm-1 M-1>
Active Site Residues:
Histidine (H63)
Aspartic acid (D107)
Serine (S200)
Activators: The rate of trypsinogen conversion is enhanced by using lanthanide in place of calcium ions (Gomez et al. 1974).
Inhibitors:
Pancreatic-, soybean-, lima bean-, and egg white- trypsin inhibitors (see section on Trypsin Inhibitors)
DFP
Aprotinin
Ag+
Benzamidine
EDTA
         (White and White 1997)
Applications:
Tissue dissociation, especially when combined with other enzymes such as collagenase, and elastase
Cell harvesting by “trypsinization”
Mitochondria isolation
in vitro studies of proteins
Removing monolayers of cells from plastic and glass
Various hemagglutination procedures
Sample preparation for flow cytometric DNA analysis
Tryptic mapping
Fingerprinting and sequencing work
Environmental monitoring
Reduction of cell density in tissue culture
Subculturing cells
Cleavage fusion proteins
Generating glycopeptides from purified glycoproteins 
(White and White 1997)