Recombinant trypsin solution, is an animalcomponent free trypsin solution optimized for cell dissociation. It is formulated with recombinant human trypsin expressed in E.Coli and refolded and purified by chromatography. The sequence of recombinant human trypsin is same as human trypsin 2 sequence.Recombinant trypsin, no bacteria, mycoplasma, mold and virus was found and bioburden is conform to biological standard, eliminates the risk of viruses, or other potential adventitious agents.Recombinant trypsin owns a similar kinetic of cell detachment to native trypsin. Soybean trypsin inhibitors and other inhibitors work the same way with recombinant human trypsin as they do with native trypsin.Recombinant trypsin provides high specific activity and eliminates contaminated material found in 1:250 trypsin or other animal origin trypsin.The trypsin solution, readyto-use, eliminates the risk of activity loss in the process of transport and storage. It also eliminates the contamination risk that a lyophilized powder has.Recombinant trypsin solution is formulated at an optimal concentration to dissociate adherent cells.No EDTA contained in buffer. And a good cell dissociation result can be obtained, and then eliminate EDTA influence on cell apoptosis experimental analysis. UNIT DEFINITION:One BAEE unit of trypsin activity will produce a Delta A253 of 0.001 per minute in a reaction volume of 3.0ml at pH7.6 and 25℃ (1cm light path)
