U-2 OS is a cell line with epithelial morphology that was derived in 1964 from a moderately differentiated sarcoma of the tibia of a 15-year-old, White, female osteosarcoma patient.
Specifications & Purity
Mycoplasma free
Images
U-2 OS Whole Cell Lysate (cl185708) - Western Blot All lanes: beta Actin Mouse mAb (Ab155822) at 1/2000 dilution Lane 1: U- 2 OS Whole Cell Lysate (cl185708) Lane 2: THP-1 Whole Cell Lysate (cl185703) Lane 3: MOLT-4 Whole Cell Lysate (cl155905) Lane 4: Jurkat Whole Cell Lysate (cl155897) Lane 5: HeLa Whole Cell Lysate (cl155894) Lane 6: THP-1 Whole Cell Lysate (cl185703) Lane 7: HUVEC Whole Cell Lysate (cl185700) Lane 8: TF-1 Whole Cell Lysate (cl185701) Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution Predicted band size: 42 kDa Observed band size: 41 kDa Exposure time: 53.8 s
U-2 OS Whole Cell Lysate (cl185708) - Western Blot All lanes: Recombinant PCNA Antibody (Ab120681) at 1/2000 dilution Lane 1: U- 2 OS Whole Cell Lysate (cl185708) Lane 2: THP-1 Whole Cell Lysate (cl185703) Lane 3: MOLT-4 Whole Cell Lysate (cl155905) Lane 4: Jurkat Whole Cell Lysate (cl155897) Lane 5: HeLa Whole Cell Lysate (cl155894) Lane 6: THP-1 Whole Cell Lysate (cl185703) Lane 7: HUVEC Whole Cell Lysate (cl185700) Lane 8: TF-1 Whole Cell Lysate (cl185701) Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution Predicted band size: 29 kDa Observed band size: 31 kDa Exposure time: 20.0 s
U-2 OS Whole Cell Lysate (cl185708) - Western Blot All lanes: Recombinant Hsp90 Antibody (Ab108929) at 1/2000 dilution Lane 1: U- 2 OS Whole Cell Lysate (cl185708) Lane 2: THP-1 Whole Cell Lysate (cl185703) Lane 3: MOLT-4 Whole Cell Lysate (cl155905) Lane 4: Jurkat Whole Cell Lysate (cl155897) Lane 5: HeLa Whole Cell Lysate (cl155894) Lane 6: THP-1 Whole Cell Lysate (cl185703) Lane 7: HUVEC Whole Cell Lysate (cl185700) Lane 8: TF-1 Whole Cell Lysate (cl185701) Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution Predicted band size: 90 kDa Observed band size: 90 kDa Exposure time: 53.5 s
Product Specifications
Form
Lyophilized
Reconstitution
Recommended reconstitute at 1-2 mg/mL in ddH₂O. Use at an assay dependent concentration. Ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.Lyophilized contents may appear as either a translucent film or a white pow
Storage Temp
Store at -80°C
Shipped In
Ice chest + Ice pads
Stability And Storage
Shipped on Dry Ice. Lyophilizate is recommended stored at -80℃. After reconstitution, recommended aliquot and avoid repeated freeze-thaw, store at -20℃ for short-term storage or -80℃ for long-term storage.
