Aladdin's U6 snRNA Cy3 FISH Probe can be used in conjunction with the Fluorescence in Situ Hybridization Kit for RNA to analyze the spatial and temporal distribution of U6 snRNA in cells, fixed tissues, and paraffin or frozen sections of human, Rattus rattus (black rat) [rodent], Mus pahari (shrew) [rodent], Mus caroli (Ryukyu mouse) [rodent], Mastomys coucha (southern polychaete) [rodent], and Xenopus laevis [frogs and toads] origin.This product can be used as a positive control in fluorescence in situ hybridization (FISH) assay of target RNA.This product is a 50nt single-stranded DNA sequence of U6 snRNA and can specifically recognize and hybridize U6 snRNA. It is labeled with the red fluorescent dye Cy3 at the 5' end, with a maximum excitation wavelength of 550nm and a maximum emission wavelength of 570nm.The recommended pre-hybridization and hybridization temperature for this product is 45℃. Please refer to Figure 1 for the hybridization effect of this product on U6 snRNA in Hela cells.Figure 1. The in situ hybridization of U6 snRNA in Hela cells using Aladdin's U6 snRNA Cy3 FISH Probe and Fluorescence in Situ Hybridization Kit for RNA . A&E. Hela cells in bright field; B. Negative control assay with the Cy3-U6 snRNA sense probe; C. Negative control cells stained with DAPI; D. The merge image of B and C; F. Assay result of specimen hybridized with the U6 snRNA Cy3 FISH Probe ; G. Specimen stained with DAPI; H. The merge image of F and G. Scale Bars are 20µm. This figure is for reference only, which may vary due to different experimental conditions.The concentration of this product is 1mg/ml. For fluorescence in situ hybridization, the recommended working concentration of U6 snRNA Cy3 FISH Probe is 0.5-1µg/ml.
Precautions：
This product should be protected from light to minimize the quenching of fluorescence.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.
Instructions for Use：
Please refer to the instructions of 's Fluorescence in Situ Hybridization Kit for RNA .
