UBA5-IN-1 (compound 8.5) is a selective UBA5 inhibitor with an IC 50 value of 4.0 μM. UBA5-IN-1 inhibits cell proliferation of Sk-Luci6 cancer cells with high expression levels of UBA5
In Vitro
UBA5-IN-1 (0.1-1000 μM) shows dose-dependent inhibition effect to UBA5 with an IC 50 value of 4.0 μM, and inhibits UAE and NAE with IC 50 values of 78.5 and 66.8 μM, respectively. UBA5-IN-1 (0-5 μM) inhibits UBA5 non-competitively with respect to ATP. UBA5-IN-1 (10 μM) shows no effect on ATP binding to human kinase. UBA5-IN-1 (0-50 μM; 72 h) effects cell proliferation of Sk-Luci6 cancer cells with high expression levels of UBA5. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Proliferation AssayCell Line: Sk-Luci6 cancer cells with high expression levels of UBA5 Concentration: 0-50 μM Incubation Time: 0-72 h Result: Showed selective anti-proliferative activity for Sk-Luci6 cancer cells which expressed higher UBA5 protein levels, but showed no anti-proliferative activity to MRC9 lung fibroblasts and A549 carcinoma cells.
