| Product Description | Aladdin's UltraBio™ Anti-GST Magnetic Beads are nano-scale amino magnetic beads covalently coupled with high-quality mouse monoclonal antibody that recognizes the GST tag specifically. This product can specifically bind GST-tagged proteins expressed in animals, plants, and microorganisms, and is recommended to use for immunoprecipitation (IP), co-immunoprecipitation (Co-IP), and protein purification.GST (glutathione S-transferase) plays an important role in the detoxification process, with a molecular weight of approximately 26kDa. It can be fused to the N-terminus or C-terminus of a target protein for convenient purification and detection. There are several advantages for using the GST as a tag. It can act as a chaperone to facilitate protein folding and increases the solubility of GST fusion proteins. It can be expressed in different hosts such as E. coli and yeast, and has a wide range of applications. It can well retain the antigenicity and biological activity of target proteins, which helps protect the recombinant protein from degradation by extracellular proteases. The GST tag can be easily removed if there is a site-specific protease recognition site between the GST tag and the target protein, such as PreScission Protease, TEV Protease, or Thrombin. The GST fusion protein can be purified specifically and conveniently by using Aladdin's GST Antibody (AH158), Anti-GST Magnetic Beads, UltraBio™ GST-tag Purification Resin, or GST Tag Protein Purification Kit . The fusion protein can also be easily detected, characterized, and used for IP or Co-IP analysis. Therefore, GST tags have been widely used in protein expression, purification, identification, protein-protein interaction, and functional studies. UltraBio™ Anti-GST Magnetic Beads specifically bind with GST fusion proteins. With a magnetic stand, this product can be conveniently used for protein purification and immunoassays. Please refer to Figure 1 for the workflow of IP assays with this product.Figure 1. Workflow of Immunoprecipitation (IP) with Aladdin's UltraBio™ Anti-GST Magnetic Beads .High specificity and binding capacity
Precautions： As the molecular weight of GST tag is relatively large (~26kDa), the molecular weight of the fusion protein is large and the structural complexity of the fusion protein may affect the recognition of the GST fusion protein by the Anti-GST magnetic beads. We recommend validating or adjusting conditions by preliminary experiments.As GST-tag binds very strongly to GST antibody, acidic elution may be less effective. It is recommended to use SDS-PAGE loading buffer for elution.The pH of Mag™ Anti-GST Magnetic Beads should be maintained at 6-8. Do not centrifuge, dry, or freeze the magnetic beads. Long-time exposure of the beads to a magnetic field will causes beads to aggregate.Resuspend Mag™ Anti-GST Magnetic Beads evenly in solution by gentle pipetting prior to use. Do not vortex.We recommend including both negative and positive controls when performing IP assays.Protein samples should be purified after collection as soon as possible and kept at 4℃ or on ice to minimize protein degradation or denaturation. Protein degradation can also be inhibited by adding appropriate protease and phosphatase inhibitors (, P1005/P1006, P1048/P1049, P1010/P1011, P1050/P1051).If using a vacuum pump to aspirate supernatant, the strength of the vacuum pump should be controlled properly to avoid the aspiration of magnetic beads.It is normal to see magnetic beads aggregate when an acidic solution is used for the elution, which can be prevented effectively by containing 0.1% non-ionic detergent (such as Triton X-100, Tween-20, or NP-40) without affecting the performance of this product.High concentrations of DTT, mercaptoethanol, or anidin hydrochloride might affect the binding of the target protein to this product, but it is compatible with ’s Cell Lysis Buffer for Western and IP , RIPA Lysis Buffer or NP-40 Lysis Buffer . For detailed information about the Lysis Buffers produced by , please refer to the following webpage:http://www.aladdin-e.com/support/lysis-buffer.htm.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.
Instructions for Use： 1.Preparation of protein samplesa.Lysis cells or tissues with an appropriate lysis buffer, such as Protein A Magnetic Beads1mlP2102-5ml Protein A Magnetic Beads5mlP2105-1ml Protein G Magnetic Beads1mlP2105-5ml Protein G Magnetic Beads5mlP2108-1ml Protein A+G Magnetic Beads1mlP2108-5ml Protein A+G Magnetic Beads5mlP2115-0.5ml Anti-Flag Magnetic Beads0.5mlP2115-2ml Anti-Flag Magnetic Beads2mlP2118-0.5ml Anti-Myc Magnetic Beads0.5mlP2118-2ml Anti-Myc Magnetic Beads2mlP2121-0.5ml Anti-HA Magnetic Beads0.5mlP2121-2ml Anti-HA Magnetic Beads2mlP2132-0.5ml Anti-GFP Magnetic Beads 0.5mlP2132-2ml Anti-GFP Magnetic Beads 2mlP2135-0.5ml Anti-His Magnetic Beads 0.5mlP2135-2ml Anti-His Magnetic Beads 2mlP2138-0.5ml Anti-GST Magnetic Beads0.5mlP2138-2ml Anti-GST Magnetic Beads2mlP2141-0.5ml Anti-V5 Magnetic Beads0.5mlP2141-2ml Anti-V5 Magnetic Beads2mlP2151-200μl Streptavidin Magnetic Beads200μlP2151-1ml Streptavidin Magnetic Beads1mlP2151-5ml Streptavidin Magnetic Beads5mlP2171-1ml Mouse IgG Magnetic Beads1mlP2171-5ml Mouse IgG Magnetic Beads5mlP2173-1ml Rabbit IgG Magnetic Beads 1mlP2173-5ml Rabbit IgG Magnetic Beads 5ml
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