Aladdin's UltraBio™ Anti-His Magnetic Beads are based on nano-scale amino magnetic beads covalently coupled with high-quality mouse monoclonal antibody that recognizes the hexahistidine tag (6xHis-tag) specifically. This product can specifically bind to His-tagged proteins expressed in animals, plants or microorganisms, and is recommended to be used for immunoprecipitation (IP), co-immunoprecipitation (Co-IP) or protein purification.His-tag is a peptide consisting of 6 consecutive histidine residues (HHHHHH). The nucleic acid sequence of His-tag is linked to the 5' end or 3' end of the target gene by recombinant technology to express His-tagged target proteins. His-tag has been widely used in protein expression, purification, identification, interaction and functional studies due to the following advantages
Precautions：
This product should be maintained at a pH of 6-8. Do not centrifuge, dry or freeze the magnetic beads. Long time exposure of the beads to magnetic field will cause beads to agglomerate.This product should be properly resuspended in solution by gentle pipetting prior to use. Do not vortex to avoid the denaturation of the antibody.When performing immunoprecipitation, it is recommended to set up both positive and negative controls appropriately.Protein samples should be purified as soon as possible after collection and should always be placed at 4℃ or on ice to minimize protein degradation or denaturation. Protein degradation can also be inhibited by adding appropriate protease inhibitors, such as Protease Inhibitor Cocktail for General Use , Protease and Phosphatase Inhibitor Cocktail for General Use (MS-safe, 50X) , Protease Inhibitor Cocktail for Mammalian Cell and Tissue Extracts , Protease and Phosphatase Inhibitor Cocktail for Mammalian Cell and Tissue Extracts , etc.If using a vacuum pump to aspirate the supernatant, the strength of the vacuum pump should be controlled properly to avoid the aspiration of magnetic beads.The beads may agglomerate when eluted with acidic solutions, which is normal and does not affect the normal use of the beads. 0.1% non-ionic detergent (e.g., Triton X-100, Tween-20 or NP-40) is effective in preventing agglomeration and does not affect the antibody binding efficiency of the beads.High concentrations of DTT, mercaptoethanol, guanidine hydrochloride, etc. may have a certain effect on the binding of this product to tagged proteins, but Cell lysis buffer for Western and IP , RIPA Lysis Buffer or NP-40 Lysis Buffer etc. are fully applicable. For selection guide of different lysis buffers produced by , please refer to our website at: http://www.aladdin-e.com/support/lysis-buffer.htm.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.
Instructions for Use：
1.Preparation of protein samples.a.Choose an appropriate lysis buffer for preparing cell or tissue lysates, such as Protein A Magnetic Beads 1ml P2102-5ml Protein A Magnetic Beads 5ml P2105-1ml Protein G Magnetic Beads 1ml P2105-5ml Protein G Magnetic Beads 5ml P2108-1ml Protein A+G Magnetic Beads 1ml P2108-5ml Protein A+G Magnetic Beads 5ml P2115-0.5ml Anti-Flag Magnetic Beads 0.5ml P2115-2ml Anti-Flag Magnetic Beads 2ml P2118-0.5ml Anti-Myc Magnetic Beads 0.5ml P2118-2ml Anti-Myc Magnetic Beads 2ml P2121-0.5ml Anti-HA Magnetic Beads 0.5ml P2121-2ml Anti-HA Magnetic Beads 2ml P2135-0.5ml Anti-His Magnetic Beads 0.5ml P2135-2ml Anti-His Magnetic Beads 2ml P2151-200μl Streptavidin Magnetic Beads 200μl P2151-1ml Streptavidin Magnetic Beads 1ml P2151-5ml Streptavidin Magnetic Beads 5ml P2171-1ml Mouse IgG Magnetic Beads 1ml P2171-5ml Mouse IgG Magnetic Beads 5ml
Product Specifications
Storage Temp
Store at -20°C
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ for up to 2 years, or -20℃ for long-term storage.
