Aladdin's UltraBio™ Anti-Myc Magnetic Beads are based on amino magnetic beads, with 200nm particle size, covalently coupled with high-quality mouse monoclonal antibody that recognizes the Myc peptide sequence (EQKLISEEDL). This product can specifically bind with Myc-tagged proteins expressed in animals, plants, and microorganisms, and is recommended to use for immunoprecipitation (IP), co-immunoprecipitation (Co-IP), and protein purification.The Myc-tag consisting of 10 amino acid residues (EQKLISEEDL) can be fused either to the N-terminus or the C-terminus of a target protein to facilitate protein purification and immunoassays. Myc-tag has the following advantages
Precautions：
Repeated freezing and thawing of this product up to 3 times does not cause obvious changes in its performance.The pH of Mag™ Anti-Myc Magnetic Beads should be maintained at 6-8. Do not centrifuge, dry, or freeze the magnetic beads. Long-time exposure of the beads to a magnetic field will causes beads to aggregate.Resuspend Mag™ Anti-Myc Magnetic Beads evenly in solution by gentle pipetting prior to use. Do not vortex.We recommend including both negative and positive controls when performing IP assays.Protein samples should be purified after collection as soon as possible and kept at 4℃ or on ice to minimize protein degradation or denaturation. Protein degradation can also be inhibited by adding appropriate protease inhibitors (, P1005/P1006, P1048/P1049, P1010/P1011, P1050/P1051).If using a vacuum pump to aspirate supernatant, the strength of the vacuum pump should be controlled properly to avoid the aspiration of magnetic beads.It is normal to see magnetic beads aggregate when an acidic solution is used for the elution, which can be prevented effectively by containing 0.1% non-ionic detergent (such as Triton X-100, Tween-20, or NP-40) without affecting the performance of this product.High concentrations of DTT, mercaptoethanol, or anidin hydrochloride might affect the binding of the target protein to this product, but it is compatible with Cell Lysis Buffer for Western and IP , RIPA Lysis Buffer or NP-40 Lysis Buffer http://www.aladdin-e.com/support/lysis-buffer.htm.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.
Instructions for Use：
1. Preparation of protein samples:a. Lysis cells or tissues with appropriate lysis buffer. We recommend using Anti-Flag Magnetic Beads0.5mlP2115-2ml Anti-Flag Magnetic Beads2mlP2118-0.5ml Anti-Myc Magnetic Beads0.5mlP2118-10ml Anti-Myc Magnetic Beads10mlP2118-2ml Anti-Myc Magnetic Beads2mlP2121-0.5ml Anti-HA Magnetic Beads0.5mlP2121-2ml Anti-HA Magnetic Beads2mlP9801-1mg3X Flag Peptide5mg/ml×0.2mlP9801-25mg3X Flag Peptide25mgP9801-5mg3X Flag Peptide5mg/ml×1mlP9805-1mgc-Myc Peptide5mg/ml×0.2mlP9805-25mgc-Myc Peptide25mgP9805-5mgc-Myc Peptide5mg/ml×1mlP9808-1mgHA Peptide5mg/ml×0.2mlP9808-25mgHA Peptide25mgP9808-5mgHA Peptide5mg/ml×1ml
Product Specifications
Storage Temp
Store at -20°C
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ for up to 2 years, or -20℃ for long-term storage.
