UltraBio™ Bacterial Active Protein Extraction Reagent
Product Description
Aladdin's UltraBio™ Bacterial Active Protein Extraction Reagent allows for rapid extraction of proteins in E. coli without the need of ultrasonication or high-pressure crushing. The extracted proteins are of high quality, high yield, and high activity. It can be used not only to extract soluble proteins, but also to wash and remove cell debris adhering to the surface of inclusion bodies to obtain high-purity inclusion bodies. It should be noted that this product can not be used to extract proteins from inclusion bodies.This product is optimized to be suitable for BL21 bacteria, DH5ɑ, JM109, and other similar bacteria as well.This product is a buffer solution containing 40mM Tris (pH 8.0) and a specific mild non-denaturing detergent that has been carefully selected to ensure that proteins can be extracted efficiently with activities well preserved. The extracted proteins can maintain their original spatial structures and biological activities, and can be used in a variety of biochemistry and cell biology applications, such as purification of His- or GST-tagged proteins, ELISA, Western Blot, IP, enzyme activity assay, and fluorescent protein assay, etc.This product can quickly and efficiently lyse bacteria, extracting cytosol and nuclear protein through a one-step method (15min) without ultrasonication or high-pressure crushing. Compared with other methods such as ultrasonication, high-pressure crushing and lysozyme treatment, target protein obtained using this product usually have higher yield and activity.The protein concentration of sample lysate obtained using this product can be determined with Aladdin's BCA Protein Assay Kit or the Detergent Compatible Bradford Protein Assay Kit .
