| Product Description | Aladdin’s
UltraBio™
Basement Membrane Matrix, also known as Extracellular Matrix (ECM) or Basement Membrane Extract (BME) gel, is free of lactate dehydrogenase elevating virus (LDEV). Matrix gel is widely used in angiogenesis, tumor cell migration, in vivo tumor modeling, 3D tumor cell models, and organoid studies. This product has good performance and can be used as a substitute of Corning® Matrigel®. The matrix gel is a basement membrane matrix extracted from the Engelbreth-Holm-Swarm (EHS) mouse tumors rich in extracellular matrix proteins. Besides the main component laminin, it also contains type IV collagen, heparan sulfate glycoprotein, entactin, various growth factors, tissue fibrinogen activator and trace amounts of matrix metalloproteinases.
Aladdin’s UltraBio™
product series are free of LDEV virus or other viruses of mouse and tumor origin. Under room temperature conditions, matrix gels can rapidly polymerize to form biologically active three-dimensional (3D) culture matrix that mimics the structure, composition, physical properties and functions of cellular basement membrane in vivo, thus promoting the attachment, maintenance, growth and differentiation of different cells such as vascular endothelial cells, epithelial cells, hepatocytes, neurons, melanoma cells, thyroid cells and hair follicle cells, by which cell morphology, biochemical functions, migration, invasion and gene expression can be investigated.
Aladdin’s UltraBio™ product series include Standard Formulation, Growth Factor Reduced (GFR), High Concentration (HC), High Concentration & Growth Factor Reduced (HC, GFR), for Stem Cells, for Organoids, and many other types of matrix gels with different growth factors, protein levels, and different applications. The Matrix-Gel™ products without phenol red are suitable for fluorescent and colorimetric assays. The matrix gel with growth factors reduced (GFR) is suitable for experiments requiring highly defined compositions, such as studies of cell signaling pathways and cytokines, while the matrix gel with high protein concentration (HC) is suitable for studies of angiogenesis, tumor cell migration and in vivo tumor models. An appropriate matrix gel can be selected by referring to the table below.
| Product No. |
Name |
Specification |
Package |
Source |
Protein Conc. |
VEGF Conc. |
Buffer |
Applications |
| B753082-SR-1ml |
UltraBio™ Basement Membrane Matrix |
Standard;with phenol red |
1ml |
EHS mouse tumors |
8-13mg/ml |
5.0-7.5ng/ml |
Low-sugar DMEM culture medium with 50 μg/ml gentamicin; compatible with all culture media. |
Routine cellular experiments such as 2D and 3D cell culture, angiogenesis, cell invasion, etc. Depending on the protein concentration required, it can also be used for in vivo experiments. |
| B753082-SR-5ml |
UltraBio™ Basement Membrane Matrix |
Standard;with phenol red |
5ml |
EHS mouse tumors |
8-13mg/ml |
5.0-7.5ng/ml |
Low-sugar DMEM culture medium with 50 μg/ml gentamicin; compatible with all culture media. |
Routine cellular experiments such as 2D and 3D cell culture, angiogenesis, cell invasion, etc. Depending on the protein concentration required, it can also be used for in vivo experiments. |
| B753082-SR-10ml |
UltraBio™ Basement Membrane Matrix |
Standard;with phenol red |
10ml |
EHS mouse tumors |
8-13mg/ml |
5.0-7.5ng/ml |
Low-sugar DMEM culture medium with 50 μg/ml gentamicin; compatible with all culture media. |
Routine cellular experiments such as 2D and 3D cell culture, angiogenesis, cell invasion, etc. Depending on the protein concentration required, it can also be used for in vivo experiments. |
| B753082-SR-50ml |
UltraBio™ Basement Membrane Matrix |
Standard;with phenol red |
50ml |
EHS mouse tumors |
8-13mg/ml |
5.0-7.5ng/ml |
Low-sugar DMEM culture medium with 50 μg/ml gentamicin; compatible with all culture media. |
Routine cellular experiments such as 2D and 3D cell culture, angiogenesis, cell invasion, etc. Depending on the protein concentration required, it can also be used for in vivo experiments. |
| B753082-SP-1ml |
UltraBio™ Basement Membrane Matrix |
Standard;without phenol red |
1ml |
EHS mouse tumors |
8-13mg/ml |
5.0-7.5ng/ml |
Low-sugar DMEM culture medium with 50 μg/ml gentamicin; compatible with all culture media. |
Routine cellular experiments such as 2D and 3D cell culture, angiogenesis, cell invasion, etc. Depending on the protein concentration required, it can also be used for in vivo experiments. |
| B753082-SP-5ml |
UltraBio™ Basement Membrane Matrix |
Standard;without phenol red |
5ml |
EHS mouse tumors |
8-13mg/ml |
5.0-7.5ng/ml |
Low-sugar DMEM culture medium with 50 μg/ml gentamicin; compatible with all culture media. |
Routine cellular experiments such as 2D and 3D cell culture, angiogenesis, cell invasion, etc. Depending on the protein concentration required, it can also be used for in vivo experiments. |
| B753082-SP-10ml |
UltraBio™ Basement Membrane Matrix |
Standard;without phenol red |
10ml |
EHS mouse tumors |
8-13mg/ml |
5.0-7.5ng/ml |
Low-sugar DMEM culture medium with 50 μg/ml gentamicin; compatible with all culture media. |
Routine cellular experiments such as 2D and 3D cell culture, angiogenesis, cell invasion, etc. Depending on the protein concentration required, it can also be used for in vivo experiments. |
| B753082-SP-50ml |
UltraBio™ Basement Membrane Matrix |
Standard;without phenol red |
50ml |
EHS mouse tumors |
8-13mg/ml |
5.0-7.5ng/ml |
Low-sugar DMEM culture medium with 50 μg/ml gentamicin; compatible with all culture media. |
Routine cellular experiments such as 2D and 3D cell culture, angiogenesis, cell invasion, etc. Depending on the protein concentration required, it can also be used for in vivo experiments. |
| B753082-GR-1ml |
UltraBio™ Basement Membrane Matrix |
Growth Factor Reduced;without phenol red |
1ml |
EHS mouse tumors |
8-13mg/ml |
1.0-1.5ng/ml |
Low-sugar DMEM culture medium with 50 μg/ml gentamicin; compatible with all culture media. |
With reduced interference from cytokine background, it is suitable for applications where a more highly defined gel composition is desired or gene expression is required. It can also be used for 3D cell culture, angiogenesis, cell invasion, organoid culture, etc. |
| B753082-GR-5ml |
UltraBio™ Basement Membrane Matrix |
Growth Factor Reduced;without phenol red |
5ml |
EHS mouse tumors |
8-13mg/ml |
1.0-1.5ng/ml |
Low-sugar DMEM culture medium with 50 μg/ml gentamicin; compatible with all culture media. |
With reduced interference from cytokine background, it is suitable for applications where a more highly defined gel composition is desired or gene expression is required. It can also be used for 3D cell culture, angiogenesis, cell invasion, organoid culture, etc. |
| B753082-GR-10ml |
UltraBio™ Basement Membrane Matrix |
Growth Factor Reduced;without phenol red |
10ml |
EHS mouse tumors |
8-13mg/ml |
1.0-1.5ng/ml |
Low-sugar DMEM culture medium with 50 μg/ml gentamicin; compatible with all culture media. |
With reduced interference from cytokine background, it is suitable for applications where a more highly defined gel composition is desired or gene expression is required. It can also be used for 3D cell culture, angiogenesis, cell invasion, organoid culture, etc. |
| B753082-GR-50ml |
UltraBio™ Basement Membrane Matrix |
Growth Factor Reduced;without phenol red |
50ml |
EHS mouse tumors |
8-13mg/ml |
1.0-1.5ng/ml |
Low-sugar DMEM culture medium with 50 μg/ml gentamicin; compatible with all culture media. |
With reduced interference from cytokine background, it is suitable for applications where a more highly defined gel composition is desired or gene expression is required. It can also be used for 3D cell culture, angiogenesis, cell invasion, organoid culture, etc. |
| B753082-HR-1ml |
UltraBio™ Basement Membrane Matrix |
High Concentration;without phenol red |
1ml |
EHS mouse tumors |
16-26mg/ml |
5.0-7.5ng/ml |
Low-sugar DMEM culture medium with 50 μg/ml gentamicin; compatible with all culture media. |
High protein concentration provides greater matrix stiffness and scaffold integrity. Suitable for in vivo tumorigenesis, angiogenesis, 3D cell culture and conventional cell culture. |
| B753082-HR-5ml |
UltraBio™ Basement Membrane Matrix |
High Concentration;without phenol red |
5ml |
EHS mouse tumors |
16-26mg/ml |
5.0-7.5ng/ml |
Low-sugar DMEM culture medium with 50 μg/ml gentamicin; compatible with all culture media. |
High protein concentration provides greater matrix stiffness and scaffold integrity. Suitable for in vivo tumorigenesis, angiogenesis, 3D cell culture and conventional cell culture. |
| B753082-HR-10ml |
UltraBio™ Basement Membrane Matrix |
High Concentration;without phenol red |
10ml |
EHS mouse tumors |
16-26mg/ml |
5.0-7.5ng/ml |
Low-sugar DMEM culture medium with 50 μg/ml gentamicin; compatible with all culture media. |
High protein concentration provides greater matrix stiffness and scaffold integrity. Suitable for in vivo tumorigenesis, angiogenesis, 3D cell culture and conventional cell culture. |
| B753082-HR-50ml |
UltraBio™ Basement Membrane Matrix |
High Concentration;without phenol red |
50ml |
EHS mouse tumors |
16-26mg/ml |
5.0-7.5ng/ml |
Low-sugar DMEM culture medium with 50 μg/ml gentamicin; compatible with all culture media. |
High protein concentration provides greater matrix stiffness and scaffold integrity. Suitable for in vivo tumorigenesis, angiogenesis, 3D cell culture and conventional cell culture. |
| B753082-HG-1ml |
UltraBio™ Basement Membrane Matrix |
High Concentration,Growth Factor Reduced;without phenol red |
1ml |
EHS mouse tumors |
16-26mg/ml |
1.0-1.5ng/ml |
Low-sugar DMEM culture medium with 50 μg/ml gentamicin; compatible with all culture media. |
Applications where both high protein concentration and reduced cytokine are required. |
| B753082-HG-5ml |
UltraBio™ Basement Membrane Matrix |
High Concentration,Growth Factor Reduced;without phenol red |
5ml |
EHS mouse tumors |
16-26mg/ml |
1.0-1.5ng/ml |
Low-sugar DMEM culture medium with 50 μg/ml gentamicin; compatible with all culture media. |
Applications where both high protein concentration and reduced cytokine are required. |
| B753082-HG-10ml |
UltraBio™ Basement Membrane Matrix |
High Concentration,Growth Factor Reduced;without phenol red |
10ml |
EHS mouse tumors |
16-26mg/ml |
1.0-1.5ng/ml |
Low-sugar DMEM culture medium with 50 μg/ml gentamicin; compatible with all culture media. |
Applications where both high protein concentration and reduced cytokine are required. |
| B753082-HG-50ml |
UltraBio™ Basement Membrane Matrix |
High Concentration,Growth Factor Reduced;without phenol red |
50ml |
EHS mouse tumors |
16-26mg/ml |
1.0-1.5ng/ml |
Low-sugar DMEM culture medium with 50 μg/ml gentamicin; compatible with all culture media. |
Applications where both high protein concentration and reduced cytokine are required. |
| B753082-QR-1ml |
UltraBio™ Basement Membrane Matrix |
hESC-Qualified;without phenol red |
1ml |
EHS mouse tumors |
8-13mg/ml |
5.0-7.5ng/ml |
Low-sugar DMEM culture medium with 50 μg/ml gentamicin; compatible with all culture media. |
Suitable for human Embryonic Stem Cells (hESC) and induced Pluripotency Stem Cell (iPSC) |
| B753082-QR-5ml |
UltraBio™ Basement Membrane Matrix |
hESC-Qualified;without phenol red |
5ml |
EHS mouse tumors |
8-13mg/ml |
5.0-7.5ng/ml |
Low-sugar DMEM culture medium with 50 μg/ml gentamicin; compatible with all culture media. |
Suitable for human Embryonic Stem Cells (hESC) and induced Pluripotency Stem Cell (iPSC) |
| B753082-QR-10ml |
UltraBio™ Basement Membrane Matrix |
hESC-Qualified;without phenol red |
10ml |
EHS mouse tumors |
8-13mg/ml |
5.0-7.5ng/ml |
Low-sugar DMEM culture medium with 50 μg/ml gentamicin; compatible with all culture media. |
Suitable for human Embryonic Stem Cells (hESC) and induced Pluripotency Stem Cell (iPSC) |
| B753082-QR-50ml |
UltraBio™ Basement Membrane Matrix |
hESC-Qualified;without phenol red |
50ml |
EHS mouse tumors |
8-13mg/ml |
5.0-7.5ng/ml |
Low-sugar DMEM culture medium with 50 μg/ml gentamicin; compatible with all culture media. |
Suitable for human Embryonic Stem Cells (hESC) and induced Pluripotency Stem Cell (iPSC) |
| B753082-FR-1ml |
UltraBio™ Basement Membrane Matrix |
For Organoid Culture;without phenol red |
1ml |
EHS mouse tumors |
8-13mg/ml |
1.0-1.5ng/ml |
Low-sugar DMEM culture medium with 50 μg/ml gentamicin; compatible with all culture media. |
Suitable for organoid studies, providing essential growth factors, proteins and required matrix structures. Commonly used in organoid growth and differentiation studies. |
| B753082-FR-5ml |
UltraBio™ Basement Membrane Matrix |
For Organoid Culture;without phenol red |
5ml |
EHS mouse tumors |
8-13mg/ml |
1.0-1.5ng/ml |
Low-sugar DMEM culture medium with 50 μg/ml gentamicin; compatible with all culture media. |
Suitable for organoid studies, providing essential growth factors, proteins and required matrix structures. Commonly used in organoid growth and differentiation studies. |
| B753082-FR-10ml |
UltraBio™ Basement Membrane Matrix |
For Organoid Culture;without phenol red |
10ml |
EHS mouse tumors |
8-13mg/ml |
1.0-1.5ng/ml |
Low-sugar DMEM culture medium with 50 μg/ml gentamicin; compatible with all culture media. |
Suitable for organoid studies, providing essential growth factors, proteins and required matrix structures. Commonly used in organoid growth and differentiation studies. |
| B753082-FR-50ml |
UltraBio™ Basement Membrane Matrix |
For Organoid Culture;without phenol red |
50ml |
EHS mouse tumors |
8-13mg/ml |
1.0-1.5ng/ml |
Low-sugar DMEM culture medium with 50 μg/ml gentamicin; compatible with all culture media. |
Suitable for organoid studies, providing essential growth factors, proteins and required matrix structures. Commonly used in organoid growth and differentiation studies. | |
The composition of this product is similar to that of conserved, early developing basement membranes. The main components of this matrix gel are approximately 60% laminin, 30% type IV collagen and 8% entactin [7-8], where entactin is a bridging molecule that interacts with laminin and type IV collagen to contribute to the formation of extracellular matrix. Besides the main components, the matrix gel also contains some proteoglycans and growth factors, such as perlecan, vascular endothelial growth factor (VEGF), transforming growth factor-β (TGF-β), epidermal growth factor (EGF), insulin-like growth factor (IGF), fibroblast growth factor (FGF), tissue fibrinogen activator (PLAT) and growth factors naturally present in tumors, as well as residual matrix metalloproteinase derived from tumor cells [9-10]. This matrix gel rapidly polymerizes at room temperature. The matrix gel will start to polymerize above 10℃. At 22-37℃, the large molecules of the matrix gel can chemically bond to each other, promoting rapid polymerization to form a 3D gel matrix. However, at low temperatures (e.g., 4℃), the matrix gel is in a liquid state because there is not enough energy to prompt chemical bonding. This matrix gel can be used for different experimental purposes. The higher the protein concentration of the matrix gel, the higher the viscosity. The protein concentration of non-high concentration matrix gel and high concentration matrix gel is 8-13mg/ml and 16-26mg/ml, respectively. The minimum protein concentration of matrix gel is 3mg/ml, but to avoid incomplete gel polymerization, the final protein concentration of matrix gel used for in vivo studies should be 4mg/ml at least. This matrix gel is subjected to strict quality control and is of high quality. Each batch of related products is strictly tested for protein concentration, endotoxin, gel, angiogenesis, 3D culture of tumor cells, organoid culture, bioactivity, and sterility (bacteria, fungi, viruses, mycoplasma), etc.
Precautions: To ensure the best performance of this product, aliquot this product at the first thawing to avoid repeated freeze-thaw. The 1ml package is also available from aladdin. This product must be stored at temperatures below -20℃ in the dark. It is not recommended to store this product in a frost-free refrigerator because the automatic defrost function of the frost-free refrigerator may affect the temperature inside the refrigerator. This product should be used aseptically to avoid microbial contamination. After thawing, place this product in a sterile area and spray 70% ethanol on the top to sterilize. Thawed matrix gels can remain liquid for about 30 days when placed in a 4℃ refrigerator or on ice in the dark, but it is still recommended to make aliquot right after thawing. Due to the actions of carbon dioxide, bicarbonate, and phenol red (if present), the color of this product may change during freeze-thaw, which is normal and have no effect on its performance. When handling this product, keep hands away from matrix gel part of the bottle to avoid polymerization due to high body temperature. Hold the bottle/tube above the matrix gel with figures. After the experiment is completed, the remaining matrix gel should not be kept for future use. This product is for R&D only. Not for drug, household, or other uses. For your safety and health, please wear a lab coat and disposable gloves during the operation. |
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