UltraBio™ Blocking Buffer(PBS)

Item Number

B751616

• Product Name: UltraBio™ Blocking Buffer(PBS)

Product Description

Instructions for Use：

1. Procedure for blocking membranesa. After completing the protein transfer, wash the membrane with Western washing buffer for 1-2 minutes.b. Based on the size of the membrane, add a sufficient volume of QuickBlock™ Blocking Buffer (PBS) in a petri dish or other appropriate container. For regular Western Blot, use 10ml of Blocking Buffer for a membrane of 6.6×8.5cm. Tween-20 or Triton X-100 can be supplemented in the Blocking Buffer to a final concentration of 0.05%-0.1% if required for the experiment. c. Clamp one corner of the membrane with a flat-tip tweezer and place the membrane in QuickBlock™ Blocking Buffer (PBS). Make sure that the membrane is covered completely by the Blocking Buffer. Incubate for 10 minutes with agitation on a horizontal shaker. The incubation time can be from 5 to 15 minutes. Generally, 10 minutes of blocking can achieve better blocking results than 1 hour’s blocking with BSA.d. The blocked membrane can be used for subsequent procedures such as primary antibody incubation. For detailed Western Blot procedures, please visit our website at http://www.aladdin-e.com/support/western.htm.2. Procedure for blocking in IF, IHC, or other experimentsIn the experimental procedures, simply replace the traditional blocking solution with QuickBlock™ Blocking Buffer (PBS) and reduce the duration of blocking to 10 minutes. If necessary, Tween-20 or Triton X-100 can be supplemented in the Blocking Buffer to a final concentration of 0.05%-0.1%. We have tested multiple primary antibodies and found that similar blocking results were obtained when the blocking time ranged from 10 minutes to 20 minutes. The results of blocking for 10 minutes with this product were equal to or significantly better than that obtained from the conventional blocking methods.Related Products:产品编号产品名称包装P0220QuickBlock™ Blocking Buffer(PBS)100mlP0222QuickBlock™ Blocking Buffer(PBSTw)100mlP0226QuickBlock™ Blocking Buffer(PBSTx)100mlP0228QuickBlock™ Blocking Buffer(TBS)100mlP0231QuickBlock™ Blocking Buffer(TBSTw)100mlP0233QuickBlock™ Blocking Buffer(TBSTx)100mlP0235QuickBlock™ Blocking Buffer(10X)100mlP0252-100mlQuickBlock™ WesternBlocking Buffer100mlP0256-100MLQuickBlock™ Primary Antibody Dilution Buffer for Western Blot100mlP0258-100MLQuickBlock™ Secondary Antibody Dilution Buffer for Western Blot100mlP0260QuickBlock™ Blocking Buffer for Immunol Staining100mlP0262QuickBlock™ Primary Antibody Dilution Buffer for Immunol Staining100mlP0265QuickBlock™ Secondary Antibody Dilution Buffer for Immunofluorescence100mlP0267QuickBlock™ Secondary Antibody Dilution Buffer for Immunohistochemistry100ml