UltraBio™ Elite Precast PAGE Gel for Tris-Gly System

Item Number

E753596

• Product Name: UltraBio™ Elite Precast PAGE Gel for Tris-Gly System

Product Description

Aladdin's UltraBio™ Elite Precast PAGE Gel for Tris-Gly System is a high-quality, ready-to-use gel in regular size. With a Tris -Glycine electrophoresis buffer, it only takes 40-60 minutes to complete electrophoresis, and very flat and sharp bands can be obtained. This precast gel has a 1.5cm high 4% stacking gel, with a very good protein separation effect. At the same time, this product has glass plates with reduced non-specific adsorptions. This product has identical or even better performance compared to home-made PAGE gels and can be routinely used for PAGE and Western analysis.

Aladdin provides a variety of UltraBio™ Elite gradient/fixed gels at different concentrations. Gradient gels are available in concentrations of 4-15%, 4-20% and 8-20%. Fixed gels include 6%, 8%, 10%, 12% and 15% gels. The gradient gels of 4-20% are most commonly used. Please refer to the table below for parameters of UltraBio™ Elite Precast PAGE Gels.

Product No.	Gel Concentration	Number of Wells	Maximum Loading Volume	Electrophoresis/Transfer Buffer System	Protein Separation Range
E753596-A1-10EA	10%	10	50μl	Tris-Glycine	20-80kDa
E753596-A1-50EA	10%	10	50μl	Tris-Glycine	20-80kDa
E753596-B1-10EA	10%	15	30μl	Tris-Glycine	20-80kDa
E753596-B1-50EA	10%	15	30μl	Tris-Glycine	20-80kDa
E753596-A2-10EA	12%	10	50μl	Tris-Glycine	12-60kDa
E753596-A2-50EA	12%	10	50μl	Tris-Glycine	12-60kDa
E753596-B2-10EA	12%	15	30μl	Tris-Glycine	12-60kDa
E753596-B2-50EA	12%	15	30μl	Tris-Glycine	12-60kDa
E753596-A3-10EA	15%	10	50μl	Tris-Glycine	10-40kDa
E753596-A3-50EA	15%	10	50μl	Tris-Glycine	10-40kDa
E753596-B3-10EA	15%	15	30μl	Tris-Glycine	10-40kDa
E753596-B3-50EA	15%	15	30μl	Tris-Glycine	10-40kDa
E753596-A4-10EA	4-15%	10	50μl	Tris-Glycine	20-180kDa
E753596-A4-50EA	4-15%	10	50μl	Tris-Glycine	20-180kDa
E753596-B4-10EA	4-15%	15	30μl	Tris-Glycine	20-180kDa
E753596-B4-50EA	4-15%	15	30μl	Tris-Glycine	20-180kDa
E753596-A5-10EA	4-20%	10	50μl	Tris-Glycine	10-180kDa
E753596-A5-50EA	4-20%	10	50μl	Tris-Glycine	10-180kDa
E753596-B5-10EA	4-20%	15	30μl	Tris-Glycine	10-180kDa
E753596-B5-50EA	4-20%	15	30μl	Tris-Glycine	10-180kDa
E753596-A6-10EA	8-16%	10	50μl	Tris-Glycine	20-90kDa
E753596-A6-50EA	8-16%	10	50μl	Tris-Glycine	20-90kDa
E753596-B6-10EA	8-16%	15	30μl	Tris-Glycine	20-90kDa
E753596-B6-50EA	8-16%	15	30μl	Tris-Glycine	20-90kDa
E753596-A7-10EA	8-20%	10	50μl	Tris-Glycine	10-90kDa
E753596-A7-50EA	8-20%	10	50μl	Tris-Glycine	10-90kDa
E753596-B7-10EA	8-20%	15	30μl	Tris-Glycine	10-90kDa
E753596-B7-50EA	8-20%	15	30μl	Tris-Glycine	10-90kDa

The ratio of acrylamide to bisacrylamide is 29:1, and the gel thickness is 1.5mm. The maximum loading volume per well of 10-well and 15-well gels is 60μl and 30μl, respectively. The gel plate size is 98×84×4.1mm (width × height × thickness) with a gel size of 81×74×1.5mm (width × height × thickness).

Polyacrylamide gel electrophoresis (PAGE) technology is widely used in protein separation, purification, detection, identification, molecular weight determination and other experiments. It is one of the most basic experimental techniques in life science research.

Unlike conventional Tris-Glycine gels, UltraBio™ Elite PAGE Gels are prepared with a slightly alkaline buffer without SDS, and can be used for both denaturing and native PAGE.

Selection of 10-well and 15-well precast gels: Protein bands obtained by 10-well precast gels are flatter and sharper, but when the number of samples to be examined is large or protein quantification is required, we recommend using the 15-well precast gel which has greater throughput and is more convenient for statistical quantitation analysis.

This product is safe and convenient to use. There is no need for gel preparation and casting, thus avoiding contact with toxic and irritating reagents. After electrophoresis, the gel can be easily removed from the glass plates.

The quality of this product is stable, with high consistency between batches.

This product has good protein resolution performance. Proteins in the gel can be transferred efficiently.

The electrophoresis time is short. The electrophoresis can be completed in 40-60 minutes at a 180V voltage or 60-70 minutes at 150V. The run time varies depending on experimental requirements. 

This product has a long shelf life. It can be stored at 4℃ for up to 12 months. As shown in Figure 1, the product shows no significant change of performance after being stored at 37℃ for 6 days.

 

Figure 1. Electrophoresis of prestained protein ladders resolved on UltraBio™ Elite PAGE Precast Gels (Tris-Gly) stored at 4℃ or at 37℃ for 6 days. Aladdin's Prestained Protein Ladders  were resolved on 4-20% or 10% UltraBio™ Elite PAGE Precast Gels (Tris-Gly) at 180V for 40 minutes. This figure is for reference only, which may vary due to different experimental conditions. 

Precautions：

This precast gel is transported at room temperature. It should not be stored at room temperature (around 22℃) for more than one month. When stored at room temperature, it should be placed in a cool place to avoid drastic temperature changes and direct sunlight.

The electrophoresis buffer in the inner chamber of the electrophoresis tank and the transfer buffer should be freshly prepared. Low-quality buffers, recycled buffers, or buffers that have been kept for too long period of time will result in poor protein resolution and transfer.

This product is for R&D only. Not for drug, household, or other uses.

For your safety and health, please wear a lab coat and disposable gloves during the operation.