UltraBio™ Plus Precast PAGE Gel(Hepes)

Item Number

P753525

• Product Name: UltraBio™ Plus Precast PAGE Gel(Hepes)

Product Description

UltraBio™ Plus Precast PAGE Gel(Hepes) is a high-quality, ready-to-use gel in regular size. With a Hepes electrophoresis buffer, it only takes 40-50 minutes to complete electrophoresis, and very flat and sharp bands can be obtained. This precast gel has a 1.5cm high 4% stacking gel, with a very good protein separation effect. At the same time, this product uses glass plates to reduce non-specific adsorptions. This product has identical or even better performance than home-made PAGE gels and can be routinely used for PAGE and Western analysis.

Aladdin provides a variety of UltraBio™ precast gradient/fixed gels of different concentrations with 10 or 15 loading wells. Gradient gels are available in concentrations of 4-15%, 4-20% and 8-20%. Fixed gels include 6%, 8%, 10%, 12% and 15% gels. The gradient gels of 4-20% are most commonly used. Please refer to the table below for the optimal protein separation range for each type of UltraBio™ Plus Precast PAGE Gels (Hepes).

Product No.	Gel Concentration	Number of Wells	Maximum Loading Volume	Electrophoresis Buffer System	Membrane Transfer Buffer System	Optimal Separation Range
P753525-A1-10EA	6%	10	60μl	Hepes	Tris-Glycine	50-150kD
P753525-A1-50EA	6%	10	60μl	Hepes	Tris-Glycine	50-150kD
P753525-B1-10EA	6%	15	30μl	Hepes	Tris-Glycine	50-150kD
P753525-B1-50EA	6%	15	30μl	Hepes	Tris-Glycine	50-150kD
P753525-A2-10EA	8%	10	60μl	Hepes	Tris-Glycine	30-90kD
P753525-A2-50EA	8%	10	60μl	Hepes	Tris-Glycine	30-90kD
P753525-B2-10EA	8%	15	30μl	Hepes	Tris-Glycine	30-90kD
P753525-B2-50EA	8%	15	30μl	Hepes	Tris-Glycine	30-90kD
P753525-A3-10EA	10%	10	60μl	Hepes	Tris-Glycine	20-80kD
P753525-A3-50EA	10%	10	60μl	Hepes	Tris-Glycine	20-80kD
P753525-B3-10EA	10%	15	30μl	Hepes	Tris-Glycine	20-80kD
P753525-B3-50EA	10%	15	30μl	Hepes	Tris-Glycine	20-80kD
P753525-A4-10EA	12%	10	60μl	Hepes	Tris-Glycine	12-60kD
P753525-A4-50EA	12%	10	60μl	Hepes	Tris-Glycine	12-60kD
P753525-B4-10EA	12%	15	30μl	Hepes	Tris-Glycine	12-60kD
P753525-B4-50EA	12%	15	30μl	Hepes	Tris-Glycine	12-60kD
P753525-A5-10EA	15%	10	60μl	Hepes	Tris-Glycine	10-40kD
P753525-A5-50EA	15%	10	60μl	Hepes	Tris-Glycine	10-40kD
P753525-B5-10EA	15%	15	30μl	Hepes	Tris-Glycine	10-40kD
P753525-B5-50EA	15%	15	30μl	Hepes	Tris-Glycine	10-40kD
P753525-A6-10EA	4-15%	10	60μl	Hepes	Tris-Glycine	20-180kD
P753525-A6-50EA	4-15%	10	60μl	Hepes	Tris-Glycine	20-180kD
P753525-B6-10EA	4-15%	15	30μl	Hepes	Tris-Glycine	20-180kD
P753525-B6-50EA	4-15%	15	30μl	Hepes	Tris-Glycine	20-180kD
P753525-A7-10EA	4-20%	10	60μl	Hepes	Tris-Glycine	10-180kD
P753525-A7-50EA	4-20%	10	60μl	Hepes	Tris-Glycine	10-180kD
P753525-B7-10EA	4-20%	15	30μl	Hepes	Tris-Glycine	10-180kD
P753525-B7-50EA	4-20%	15	30μl	Hepes	Tris-Glycine	10-180kD
P753525-A8-10EA	8-20%	10	60μl	Hepes	Tris-Glycine	10-90kD
P753525-A8-50EA	8-20%	10	60μl	Hepes	Tris-Glycine	10-90kD
P753525-B8-10EA	8-20%	15	30μl	Hepes	Tris-Glycine	10-90kD
P753525-B8-50EA	8-20%	15	30μl	Hepes	Tris-Glycine	10-90kD

The ratio of acrylamide to bisacrylamide is 29:1, and the gel thickness is 1.5mm. The maximum loading volume per well of 10-well and 15-well gels is 60μl and 30μl, respectively. The plate size is 98×84×4.1mm (width × height × thickness), with a gel size of 81×74×1.5mm (width × height × thickness).

Polyacrylamide gel electrophoresis (PAGE) technology is widely used in protein separation, purification, detection, identification, molecular weight determination and other experiments. It is one of the most basic experimental techniques in life science research.

Unlike traditional Tris-Glycine gels, UltraBio™ Plus PAGE precast gels for Hepes system are prepared with neutral pH Hepes buffer and do not contain SDS. They can be used for both denaturing and native protein electrophoresis.

After electrophoresis, protein can be transferred from the gel to blot membrane with the Tris-Glycine buffer system.

Selection of 10-well and 15-well precast gels: Protein bands obtained by 10-well precast gels are flatter and sharper, but when the number of samples to be examined is large or protein quantification is required, we recommend using the 15-well precast gel which has greater throughput and is more convenient for statistical quantitation analysis.

This product is safe and convenient to use. There is no need for gel preparation and casting, thus avoiding contact with toxic and irritating reagents. After electrophoresis, the gel can be easily removed from the glass plates.

The quality of this product is stable, with high consistency between batches.

This product has good protein resolution performance. Proteins in the gel can be transferred efficiently as well.

The electrophoresis time is short. The electrophoresis can be completed in 40-50 minutes at a 150V voltage. The run time varies depending on experimental requirements.

Precautions:

Do NOT freeze this product at temperatures below 0℃.

The electrophoresis buffer in the inner chamber of the electrophoresis tank and the transfer buffer should be freshly prepared. Low-quality buffers, recycled buffers, or buffers that have been kept for too long period of time will result in poor protein resolution and transfer.

This product is for R&D only. Not for drug, household, or other uses.

For your safety and health, please wear a lab coat and disposable gloves during the operation.