| Product Description | Aladdin's UltraBio™ Primary Antibody Dilution Buffer for Western Blot can enhance the specific binding between antigen and antibody in Western assay, thus improving the sensitivity of Western assay up to more than ten times. This product can also be used for sensitization of other immunoassays involving antigen-antibody binding such as Immunofluorescence, Immunohistochemistry, Dot Blot and ELISA.Western blot is one of the main methods to detect protein expression level with antibodies. The main procedures include SDS-PAGE electrophoresis, membrane transfer, blocking, primary antibody incubation, secondary antibody incubation and bot development. For detailed Western blot procedures, please refer to http
Precautions： To ensure the best results, we recommend using this product in conjunction with Quickblock™ Western Blocking Buffer or Blocking Buffer for Western Blot , and Wash Buffer for Western Blot .A small amount of white precipitates may occur in this product after stored at low temperatures or frozen at -20°C. Before use, the precipitates should be completely dissolved by incubating the bottle in a 30-50°C water bath for an appropriate period of time.Primary antibodies diluted and prepared with this product can be stored at 4ºC for multiple uses in Western blot, but should be discarded when there is turbidity, precipitation, or a decrease of assay efficacy.Diluted antibodies should be stored at 4ºC immediately after use to enable multiple uses.This product is recommended for primary antibody dilution, but not for blocking membranes nor coating antigens or antibodies in ELISA.For primary antibody dilution, there is no need to add other blocking reagents such as BSA, skim milk powder, and casein, as this product has been optimized to have good blocking effect. The addition of other blocking reagents may have some negative effects.The effect of this product varies for different target proteins and antibodies.If the signal of the target protein is very strong, the non-specific signals will be enhanced accordingly. If the non-specific signal intensity is very close to that of target bands, attempt to reduce the protein loading volume, decrease the concentration of antibody, or shorten the exposure time appropriately.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.
Instructions for Use： 1. Dilute the primary antibody with this product at an appropriate ratio according to manufacture's instructions of the antibody used and the content of target protein in samples. The diluted primary antibody can be recovered and stored at 4ºC for later use in Western blot. 2. For detailed procedures of Western blot, please refer to http://www.aladdin-e.com/support/western.htm.Related Products:产品编号产品名称包装E6001MiniProGel™ Protein Gel-Cast and Electrophoresis System (4-gel)1SetE6005MiniProGel™ Protein Gel-Cast and Electrophoresis System (2-gel)1SetE6050MiniBlot™ Electrophorectic Transfer Cell1SetE6080 Elite Power Supply (300V/600mA/100W)1SetE6085 Prime Power Supply (300V/2000mA/200W)1SetE6150MiniProGel™ Protein Gel-Cast, Electrophoresis and Transfer System (2-gel/2-blot)1SetE6155MiniProGel™ Protein Gel-Cast, Electrophoresis and Transfer System (4-gel/2-blot)1SetE6159MiniProGel™ Protein Gel-Cast, Electrophoresis and Transfer System (4-gel/4-blot)1SetE6600TissueMaster™ Handheld Homogenizer1SetEI600 600 Chemiluminescent Imaging System1SetFFP24PVDF Membrane (6.6×8.5cm, 0.2μm)20 pcs/PackageFFP32PVDF Membrane (6.6×8.5cm, 0.45μm)20 pcs/PackageFFP51Filter Paper for Blotting Transfer100 pcs/PackageFFX050Western Membrane Washing Box (9.0×6.0×3.3cm)1/PackageP0006Bradford Protein Assay Kit1000TP0012BCA Protein Assay Kit500TP0012ASDS-PAGE Gel Preparation Kit30-50 gelsP0012ACSDS-PAGE Gel Quick Preparation Kit30-50 gelsP0013Cell Lysis Buffer for Western or IP100mlP0013BRIPA Lysis Buffer 100mlP0013DRIPA Lysis Buffer (Weak)100mlP0014ASDS-PAGE Electrophoresis Buffer with Tris-Gly, Powder1LP0014BSDS-PAGE Electrophoresis Buffer with Tris-Gly, Powder10LP0014CSDS-PAGE Electrophoresis Buffer with Tris-Gly, 10X100mlP0014DSDS-PAGE Electrophoresis Buffer with Tris-Gly, 10X500mlP0015SDS-PAGE Sample Loading Buffer (5X)2mlP0015FSDS-PAGE Sample Loading Buffer (6X)2mlP0015LSDS-PAGE Sample Loading Buffer (5X)15mlP0017F Coomassie Blue Super Fast Staining Solution250mlP0018M Prestained Color Protein Marker, or Prestained Color Protein Ladder200μlP0078 Prestained Color Protein Marker, or Prestained Color Protein Ladder200μlP0097-100MLEnhanced Immunostaining Permeabilization Buffer100mlP0098-100MLImmunol Staining Fix Solution100mlP0099-100ML4% Paraformaldehyde Fix Solution, 4% PFA Fix Solution100mlP0102Immunol Staining Blocking Solution100mlP0103Immunol Staining Primary Antibody Dilution Solution100mlP0106Immnol Staining Wash Buffer250mlP0108Immnol Fluorescence Staining Secondary Antibody Dilution Solution100mlP0220QuickBlock™ Blocking Buffer(PBS)100mlP0235QuickBlock™ Blocking Buffer(10X)100mlP0252-100MLQuickBlock™ WesternBlocking Buffer100mlP0256-100MLQuickBlock™ Primary Antibody Dilution Buffer for Western Blot100mlP0258-100MLQuickBlock™ Secondary Antibody Dilution Buffer for Western Blot100mlP0260QuickBlock™ Blocking Buffer for Immunol Staining100mlP0262QuickBlock™ Primary Antibody Dilution Buffer for Immunol Staining100mlP0265QuickBlock™ Secondary Antibody Dilution Buffer for Immunofluorescence100mlP0267QuickBlock™ Secondary Antibody Dilution Buffer for Immunohistochemistry100mlP0272-100MLSignalUp™ Western Blot Enhancer100mlP0273-100MLSignalUp™ Primary Antibody Dilution Buffer for Western Blot100mlP0274-100MLSignalUp™ Secondary Antibody Dilution Buffer for Western Blot100mlP0276-100MLSignalUp™ Immunostaining Enhancer100mlP0277-100MLSignalUp™ Primary Antibody Dilution Buffer for Immunostaining100mlP0278-100MLSignalUp™ Secondary Antibody Dilution Buffer for Immunostaining100mlP0280InstantView™ SDS-PAGE Protein Staining and Loading Buffer (5X)1mlP0448S Plus Precast PAGE Gel for Tris-Gly System10P0472S Plus Precast PAGE Gel for Tris-Gly System10P0501S Plus Precast PAGE Gel(6%, 10well)10P0528S Plus Precast PAGE Gel(8-20%, 15well)10P0552-100ML Plus SDS-PAGE Hepes Electrophoresis Buffer100mlP1005Protease inhibitor cocktail for general use, 100X1mlP1031Protease inhibitor cocktail for purification of His-tagged proteins, 100X5mlP1045Protease and phosphatase inhibitor cocktail for general use , 50X2mlP1066Protease and phosphatase inhibitor cocktail for bacterial cell extracts, 50X10mlP1081Phosphatase inhibitor cocktail A, 50X2mlP1097Phosphatase inhibitor cocktail D ,50X10mlP1112Deacetylase Inhibitor Cocktail,100X1mlP1113Deacetylase Inhibitor Cocktail ,100X5ml
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