| Product Description | Aladdin's UltraBio™ Protein G Plus Magnetic Beads are based on nano-scale amino magnetic beads covalently coupled with optimized high-quality recombinant Protein G. Compared with UltraBio™ Protein G Magnetic Beads , Protein G Plus Magnetic Beads have stronger ability to specifically bind the corresponding antibodies. With the same amount of magnetic beads, Protein G Plus Magnetic Beads can bind about 30-50% more antibodies. Protein G Plus Magnetic Beads are mainly used for immunoprecipitation (IP), co-immunoprecipitation (Co-IP) or chromatin immunoprecipitation (Ch-IP), and can also be used for antibody purification from serum, cell culture supernatant, or ascites fluid.Protein G is an immunoglobulin binding protein expressed by Streptococcal bacteria type C or G. Protein A is a cell wall surface protein found in Staphylococcus aureus with a molecular weight of 42kDa. Protein A and Protein G are functionally similar and can bind specifically to mammalian immunoglobulin (Ig), usually to the Fc region of the immunoglobulin, but it has been reported that Protein A also binds to the Fab region of the human VH3 family, and Protein G sometimes binds to the Fab region as well. At the same time, Protein A and Protein G differ in their ability to bind to different immunoglobulin subclasses. Recombinant Protein A and G with appropriate modifications bound in a certain way to magnetic beads can be used for immunoprecipitation or antibody purification. The Protein G Plus used in this magnetic bead optimizes the steric hindrance effect of Protein G to bind 3 IgG molecules at the same time without changing the basic performance of Protein G, and has a stronger ability to specifically bind the corresponding antibody. With the same amount of magnetic beads in some cases, Protein G Plus magnetic beads can bind approximately 30-50% more antibodies.Like Protein G Magnetic Beads, Protein G Plus Magnetic Beads are suitable for immunoprecipitation of human IgG1,IgG2,IgG3,IgG4,mouse IgG1,IgG2a,IgG2b,IgG3,rat IgG1,IgG2a,IgG2b, IgG2c,and polyclonal antibodies from rabbit and goat. Protein A magnetic beads are suitable for immunoprecipitation of human IgG1,IgG2,IgG4,mouse IgG2a,IgG2band rabbitrabbit IgG. The following table shows the binding capability of Aladdin's Protein A, Protein G and Protein A/G magnetic beads to common immunoglobulin subclasses of human, mouse and rat, and the total binding capacity of different species.SpeciesIgProtein AProtein GA+GHumanIgG1++++++++++++IgG2++++++++++++IgG3-++++++++IgG4++++++++++++IgA++-++IgD++-++IgE++-++IgM++-++MouseIgG1+++++++++IgG2a++++++++++++IgG2b+++++++++IgG3++++++++IgM+/--+/-RatIgG1-++IgG2a-++++++++IgG2b-++++IgG2c+++++IgM+/--+/-Total IgProtein AProtein GA+GHuman++++++++++++Mouse+++++++++Rat+/-++++Rabbit+++++++++++Goat-++++Chicken-++Cow++++++++++Guinea Pig++++++++++Hamster+++++Horse++++++++++Pig+++++++++Sheep+/-++++ ++++, Strong Binding ++~+++, Medium Binding +, Weak Binding +/-, Weak or No Binding -, No BindingThe recombinant Protein G Plus in this product binds specifically to the Fc region of most mammalian IgGs and has a molecular weight of approximately 25kDa. The recombinant Protein G Plus has been modified to reduce non-specific binding by retaining only the amino acid sequences that bind with the Fc region of IgG. Each Protein G Plus molecule can bind up to 3 IgG molecules.UltraBio™ Protein G Plus Magnetic Beads can specifically bind to the relevant antibodies and can be applied to experiments such as immunoprecipitation or purification of target proteins or their protein complexes with the aid of a magnetic stand. Please refer to Figure 1 for the flow chart of this product for immunoprecipitation.Figure 1. The flow chart for immunoprecipitation with Aladdin's UltraBio™ Protein G Plus magnetic beads .High specificity and high binding capacity
Precautions： It has been tested that repeated freeze-thaws for more than 3 times will not affect the performance of this product.This product should be maintained at a pH of 6-8. Do not centrifuge, dry or freeze the magnetic beads. Long time exposure of the beads to magnetic field will cause beads to agglomerate.This product should be properly resuspended in solution by gentle pipetting prior to use. Do not vortex to avoid the denaturation of the antibody.When performing immunoprecipitation, it is recommended to set up both positive and negative controls appropriately.Protein samples should be purified as soon as possible after collection and should always be placed at 4℃ or on ice to minimize protein degradation or denaturation. Protein degradation can also be inhibited by adding appropriate protease inhibitors, such as Protease Inhibitor Cocktail for General Use ,Protease and Phosphatase Inhibitor Cocktail for General Use (MS-safe, 50X) , Protease Inhibitor Cocktail for Mammalian Cell and Tissue Extracts , Protease and Phosphatase Inhibitor Cocktail for Mammalian Cell and Tissue Extracts , etc.If using a vacuum pump to aspirate the supernatant, the strength of the vacuum pump should be controlled properly to avoid the aspiration of magnetic beads.The beads may agglomerate when eluted with acidic solutions, which is normal and does not affect the normal use of the beads. 0.1% non-ionic detergent (e.g., Triton X-100, Tween-20 or NP-40) is effective in preventing agglomeration and does not affect the antibody binding efficiency of the beads.High concentrations of DTT, mercaptoethanol, guanidine hydrochloride, etc. may have a certain effect on the binding of this product to tagged proteins, but Cell lysis buffer forWestern and IP RIPA Lysis Buffer orNP-40 Lysis Buffer etc. are fully applicable. For selection guide of different lysis buffers produced by , please refer to our website at: http://www.aladdin-e.com/support/lysis-buffer.htm。This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.
Instructions for Use： 1.Preparation of protein samples.a.Choose an appropriate lysis buffer for preparing cell or tissue lysates, such as Protein A Magnetic Beads 1ml P2102-5ml Protein A Magnetic Beads 5ml P2105-1ml Protein G Magnetic Beads 1ml P2105-5ml Protein G Magnetic Beads 5ml P2106-1ml Protein G Plus Magnetic Beads 1ml P2106-5ml Protein G Plus Magnetic Beads 5ml P2108-1ml Protein A+G Magnetic Beads 1ml P2108-5ml Protein A+G Magnetic Beads 5ml
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