Aladdin's UltraBio™ Red Fluorescent DNA Loading Buffer (6X, Bromophenol Blue) is used for loading DNA samples onto agarose or polyacrylamide gels for electrophoresis. It contains NA-Red (Nucleic Acid Red) fluorescent dye that binds to nucleic acids at the moment of mixing and emits red fluorescence under UV light.This product is easy to use. A simple three-step procedure includes adding 1/6 volume of 6X DNA Loading Buffer to DNA, loading onto gel and electrophoresis. There is no need to add any dye in agarose gels or stain gels after electrophoresis.This product is safe, sensitive, and highly stable. NA-Red is a significantly safer alternative to ethidium bromide, with very low mutagenicity and no cytotoxicity. It allows the detection of ＞0.6ng of DNA, and has good thermal and photo-stability. Please refer to Figure 1 for the sensitivity of this product.Figure 1. Image of different amounts of DNA loaded with Aladdin's UltraBio™ Red Fluorescent DNA Loading Buffer (6X, Bromophenol Blue) on agarose gel after electrophoresis. The actual results may vary due to differences in DNA quality and detection instrument used.Imaging System documented with the same filters as ethidium bromide can be used. Red fluorescence is detected at a wavelength of about 300nm, which is very close to the excitation and emission light of EB.This product uses bromophenol blue as an indicator for easy visual tracking of DNA migration during electrophoresis. Bromophenol blue comigrates with 200-500 bp DNA fragments, which has more or less interference with the observation of DNA fragments around this size. When it happens, please use Aladdin's UltraBio™ Red Fluorescent DNA Loading Buffer (6X, Red).UltraBio™™ Red Fluorescent DNA Ladders should be used in parallel with this product. You can also use the regular DNA ladder supplemented with an appropriate amount of Aladdin's UltraBio™ Red Fluorescent DNA Marker dye (50X). Please refer to Figure 2 for the stain result of DNA samples loaded with this product and the UltraBio™ Red Fluorescent DNA Ladder .Figure 2. Image of the UltraBio™ Red Fluorescent DNA Ladder and DNA samples of different lengths loaded with Aladdin's UltraBio™ Red Fluorescent DNA Loading Buffer (6X, Bromophenol Blue) on agarose gel after electrophoresis.
Precautions：
This product should be kept in the dark. Exposure to regular indoor light during the electrophoresis does not cause any change in its performance.This product can be added directly to DNA samples for loading and electrophoresis. Agarose gels after electrophoresis are not recommended for reuse.The gel can be observed directly after electrophoresis using a gel imager. If the background is too high, the gel can be washed with nuclease-free water to reduce the background.This product can stain dsDNA, ssDNA, and RNA.When smear or unsatisfactory band separation is observed, the following methods can be attempted: use freshly prepared electrophoresis buffer; reduce the loading amount of DNA; reduce the agarose concentration; use a longer gel; decrease the voltage for electrophoresis and extend the time of electrophoresis to improve DNA separation, etc.This product is compatible with commonly used electrophoresis buffers, such as TAE and TBE.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.
Instructions for Use：
1. Prepare agarose gel with an appropriate concentration. Do not add any dye in the gel.2. Mix the 6X DNA Loading Buffer well before use. Add 1/6 volume of 6X DNA Loading Buffer to your DNA sample, mix well and load directly onto agarose gel for electrophoresis. Note 1: InstantView™ Red Fluorescent DNA Ladders ( Red Fluorescent DNA Loading Buffer (6X, Red) from .3. After electrophoresis, use a Gel Imager System for observation or taking photos.
