| Product Description | Aladdin's UltraBio™ Transfer Buffer (20X, for Bis-Tris Gels), also known as UltraBio™ Bis-Tris Adhesive Transfer Liquid (20X), is a 20X concentrated solution for wet transfer during Western blot. This product is specially designed for use with Bis-Tris gels, but can also be used for Tris-Acetate gels.This product can maintain a neutral pH environment during the transfer, thereby protecting the amino acid side chain from modification. It is compatible with the Edman degradation technology to determine the N-terminal amino acid sequences of proteins [1].When transferring reducing proteins, it is recommended to add 0.1% of Aladdin's UltraBio™ Antioxidant (400X) in this transfer buffer to promote the transfer of reducing proteins.This product is a 20X concentrated solution prepared with high-quality Bicine, Bis-Tris, EDTA and other reagents. It can be used for protein transfer after being diluted to 1X with distilled water, without the need of pH retitration. Investigators can also purchase the UltraBio™ Transfer Buffer (Powder, for Bis-Tris Gels) for use.For protein transfer from Bis-Tris and Tris-Acetate gels, it is also feasible to use the Western Transfer Buffer with the Tris-Glycine buffer system.This transfer buffer can be recycled for 1-2 times.
Precautions： We do not recommend transferring reducing protein samples and non-reducing protein samples together. For transferring reducing proteins, it is recommended to add 0.1% Antioxidant (400X) (, P0737) in the transfer buffer.Transfer buffer containing 10% ethanol or methanol enables optimal protein transfer of a single gel. For simultaneous protein transfer from two gels, increase the ethanol or methanol content to 20% to ensure efficient transfer of proteins.This product is suitable for Bis-Tris and Tris-Acetate gel systems, not suitable for gels with other buffer systems.If the current or voltage during the transfer is high, the generated Joule heating leads to a lower resistance of the transfer buffer, which ultimately affects the transfer efficiency, the buffering capacity of the transfer buffer, and even causes the gel to melt or adhere to the membrane. Therefore, we recommend performing the transfer in an ice box or at 4℃.When using this product for protein transfer and setting the constant current at 400mA, the voltage is generally 150-200V at the beginning no matter there is 1 or 2 pieces of membrane in the transfer tank. As the temperature of the transfer buffer will gradually increase and the resistance will become smaller, the voltage is generally 100-150V at the end. The transfer efficiency is related to the electric field strength (E) which is mainly related to the voltage and the distance between the electrodes, i.e., E=U/d.If an error occurs during the transfer, check whether it is an overload of voltage or current, or abnormal conditions such as too much or too little transfer buffer, short circuit or breakage of the transfer tank, etc. Some power supplies will automatically reduce the voltage or current, while others will report an error and stop running. When the transfer stops, it is necessary to reduce the current or voltage, or choose a power supply with more power.The temperature of the transfer buffer during the transfer, the parameters of the gel (e.g., number, thickness, ionic concentration, acrylamide concentration, etc.), the thickness of the filter paper, the number of transfer tanks used simultaneously, the ionic concentration of the transfer buffer, the volume of the transfer buffer, and the number of times the transfer buffer is used may all affect the current and voltage. Among them, the number of simultaneous transfer tanks used for one electrophoresis is the most critical factor. In case of constant current, each additional transfer tank will reduce the current and voltage of each tank accordingly. If 2 or more transfer tanks need to be used at the same time, it is recommended to use a high power supply with high current, or set a lower current or voltage for a longer transfer time.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.
Instructions for Use： 1. Prepare the transfer buffer according to the table below.ReagentReduced SamplesNon-Reduced Samples Transfer Buffer(20X, for Bis-Tris Gels)50ml50ml Antioxidant (400X) 1ml-Ethanol or Methanol100-200ml100-200mlH2OTo 1000mlTo 1000mlTotal Volume1000ml1000mlNote: Better transfer result can be obtained when using the ice-cold transfer buffer for the transfer. Unused transfer buffer can be stored at room temperature or 4℃ for up to two weeks, but should be discarded when its color turns light brown or yellowish brown.2. Transfer conditions: Protein transfer can be completed in one hour at 300mA constant current or 100V constant voltage (external ice bath is recommended). The transfer time can be appropriately reduced for small molecular weight proteins or increased for larger proteins. This transfer buffer is also suitable for rapid transfer, such as 500mA constant current for 15 minutes.2. For detailed Western procedures, please refer to http://www.aladdin-e.com/support/western.htm.Related Products:产品编号产品名称包装P0731-10ml LDS Sample Buffer (4X)10mlP0731-2ml LDS Sample Buffer (4X)2mlP0733-10ml Sample Reducing Agent (10X)10mlP0733-2ml Sample Reducing Agent (10X)2mlP0737-10ml Antioxidant (400X)10mlP0745-100ml MES SDS Running Buffer (20X)100mlP0745-500ml MES SDS Running Buffer (20X)500mlP0750-2ml Tricine SDS Sample Buffer (2X)2mlP0752-100ml Tricine-SDS Anode Running Buffer (10X)100mlP0753-100ml Transfer Buffer (20X, for Bis-Tris Gels)100mlP0755-1L Transfer Buffer (Powder, for Bis-Tris Gels)1L
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