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Urease from Canavalia ensiformis (Jack bean) - ≥45 units/mg dry weight, high purity , CAS No.9002-13-5

Item Number
U128713
Grouped product items
SKUSizeAvailabilityPrice Qty
U128713-250mg
250mg
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$408.90
U128713-1g
1g
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$1,142.90
U128713-5g
5g
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$3,999.90
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hydrolase (21) All Research Area

Basic Description

Specifications & PurityEnzymoPure™, ≥45 units/mg dry weight
Biochemical and Physiological MechanismsUrease catalyzes the hydrolysis of urea into carbon dioxide and ammonia. Urease is involved in nitrogen metabolism and urea degradation. Urease from Canavalia ensiformis binds 2 nickel ions per subunit.
Storage TempStore at -20°C
Shipped InIce chest + Ice pads
GradeEnzymoPure™
Product Description

Jespersen (1975) reports that ammonium carbamate is produced in citrate and Tris buffer.
Urease occurs in many bacteria, several species of yeast and a number of higher plants. Varner (1960) has reviewed it. Two of the best sources are: Jack beans (Canavlia ensiformis) from which it has been crystallized and thoroughly studied, and Bacillus pasteurii.
The enzyme is important in assaying for urea. See Guilbault and Montalvo (1970). Its immobilization has been reported: et al. (1974), James and Pring (1975), Messing (1974), Nakamoto et al. (1975), Sundaram (1973) and Tran-Minh and Broun (1975).
Characteristics of Urease from Jack Bean:
Km: 1.3mM in Tris⋅HCl (Cesareo and Langton, 1992).
Specificity
Urease is specific for urea and hydroxyurea (Fishbein and Carbone 1965). See also Sundaram and Laidler (1970).
Composition
Monomeric (a)urease can polymerize to form six unit polymers of about three million daltons. (Fishbein et al. 1970; Fishbein and Nagarajan 1972a). Andrews and Reithel (1970) report on the sulfhydryl groups. Contaxis and Reithel (1971) indicate the molecule can be split in half with no loss of activity. See also Contaxis and Reithel (1972), Fishbein and Nagarajan (1972b), Lynn (1970), and Bailey and Boulter (1969).
Optimal pH:7.4 
Stabilizers
EDTA in concentrations of 1 X 10-3 M. 50% glycerol solutions protect urease crystalline suspension for several months at 4°C.
Inhibitors
Heavy metals. NH4+ ions formed. See also Fishbein and Carbone (1965). Sodium and potassium ions are inhibitors (Cesareo and Langton, 1992).
Urease Assay
Method
Worthington has adopted an assay method where the hydrolysis of urea is measured by coupling ammonia production to a glutamate dehydrogenase reaction.
formulas
One unit results in the oxidation of one micromole of NADH per minute at 25°C and pH 7.6 under the specified conditions. In addition to increased sensitivity, the assay method possesses the advantage that it can be manipulated to permit quantitation of urea.
Reagents
0.1 M Potassium phosphate buffer, pH 7.6
0.023 M Adenosine-5'-diphosphate (ADP) in phosphate buffer
0.0072 M NADH in phosphate buffer
0.026 M a-Ketoglutarate in phosphate buffer
1.8 M Urea in phosphate buffer
Glutamate Dehydrogenase: Dilute to approximately 500 units/ml in 50% glycerol or phosphate buffer. Store cold during use.
Enzyme
Dissolve enzyme at one mg/ml in 0.1 M phosphate buffer, pH 7.6. Immediately prior to use, dilute further in buffer to obtain a rate of 0.02-0.04 ΔA/minute.
Procedure
Adjust spectrophotometer to 340 nm and 25°C. Pipette into each cuvette as follows:

0.10 M Phosphate buffer, pH 7.6 2.4 ml
0.023 M ADP 0.1 ml
0.0072 M NADH 0.1 ml
0.026 M α-Ketoglutarate 0.1 ml
1.8 M Urea 0.1 ml
GLDH (500 units/ml) 0.1 ml

Incubate in spectrophotometer at 25°C for 5-10 minutes to achieve temperature equilibration and establish blank rate, if any. A slight change in absorbance may be observed due to trace ammonia in reagents. Upon obtaining a zero change in absorbance, add 0.1 ml appropriately diluted enzyme. Record decrease in A340 for 8-10 minutes. Determine ΔA340/minute from the linear portion of the curve. A slight lag may occur.

Calculation 


Names and Identifiers

Enzyme Commission Number3.5.1.5
WGK Germany 1

Certificates

C of A & Other Certificates(BSE/TSE, COO)

Find and download the COA for your product by matching the lot number on the packaging.

9 results found

Lot NumberCertificate TypeDateItem
J2409536Certificate of AnalysisSep 26, 2024 U128713
J2409537Certificate of AnalysisSep 26, 2024 U128713
J2409538Certificate of AnalysisSep 26, 2024 U128713
G2317037Certificate of AnalysisJul 06, 2023 U128713
G2317079Certificate of AnalysisJul 06, 2023 U128713
G2317096Certificate of AnalysisJul 06, 2023 U128713
G2426152Certificate of AnalysisJul 06, 2023 U128713
B2318515Certificate of AnalysisJul 20, 2022 U128713
H2209752Certificate of AnalysisJul 20, 2022 U128713

Chemical and Physical Properties

SensitivityMoisture Sensitive

Safety and Hazards(GHS)

Pictogram(s) GHS08,   GHS07
Signal Danger
Hazard Statements

H315:Causes skin irritation

H319:Causes serious eye irritation

H335:May cause respiratory irritation

H334:May cause allergy or asthma symptoms or breathing difficulties if inhaled

Precautionary Statements

P261:Avoid breathing dust/fume/gas/mist/vapors/spray.

P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do - continue rinsing.

P342+P311:IF experiencing respiratory symptoms: Call a POISON CENTER/doctor/...

WGK Germany 1

Related Documents

Citations of This Product(29)

1. Xinyu Shao, Lulu Cao, Lixia Lu.  (2024)  Ultrasensitive detection of glucose oxidase and alkaline phosphatase in milk based on valence regulated upconversion nanoprobes.  FOOD CHEMISTRY,  432  (137212).  [PMID:37634343] [10.1016/j.foodchem.2023.137212]
2. Zhao Chang, Xiao Yang, Chu Jian, Hu Ran, Liu Hanlong, He Xiang, Liu Yi, Jiang Xiang.  (2023)  Microfluidic experiments of biological CaCO3 precipitation in transverse mixing reactive environments.  Acta Geotechnica,  18  (10): (5299-5318).  [PMID:] [10.1007/s11440-023-01938-w]
3. Pengyu Xi, Xuecheng He, Chuan Fan, Qinglin Zhu, Zehua Li, Yuemeng Yang, Xin Du, Tailin Xu.  (2023)  Smart Janus fabrics for one-way sweat sampling and skin-friendly colorimetric detection.  TALANTA,  259  (124507).  [PMID:37058940] [10.1016/j.talanta.2023.124507]
4. Liangliang Cheng, Wanlu Zheng, Ya-Nan Zhang, Xuegang Li, Yong Zhao.  (2023)  Highly Sensitive Fiber-Optic SPR Urea Sensor Based on ZIF-8/Urease.  IEEE TRANSACTIONS ON INSTRUMENTATION AND MEASUREMENT,  72  (1-7).  [PMID:] [10.1109/TIM.2023.3259040]
5. Yuyang Bei, Kamile Arkin, Yuxin Zheng, Xuesong Ma, Jie Zhao, Huimin Jin, Qingkun Shang.  (2023)  Construction of a ratiometric fluorescent probe for visual detection of urea in human urine based on carbon dots prepared from Toona sinensis leaves and 5-carboxyfluorescein.  ANALYTICA CHIMICA ACTA,  1240  (340733).  [PMID:36641152] [10.1016/j.aca.2022.340733]
6. Wanying Cui, Chunmiao Xia, Sheng Xu, Xinke Ye, Yihao Wu, Shukai Cheng, Rongli Zhang, Cuige Zhang, Zongcheng Miao.  (2023)  Water-in-water emulsions stabilized by self-assembled chitosan colloidal particles.  CARBOHYDRATE POLYMERS,  303  (120466).  [PMID:36657862] [10.1016/j.carbpol.2022.120466]
7. Xuecui Mei, Jiao Yang, Jiang Liu, Yingchun Li.  (2023)  Wearable, nanofiber-based microfluidic systems with integrated electrochemical and colorimetric sensing arrays for multiplex sweat analysis.  CHEMICAL ENGINEERING JOURNAL,  454  (140248).  [PMID:] [10.1016/j.cej.2022.140248]
8. Ming Zhao, Zhenhai Chen, Li Hao, Huayao Chen, Xinhua Zhou, Hongjun Zhou.  (2023)  CMC based microcapsules for smart delivery of pesticides with reduced risks to the environment.  CARBOHYDRATE POLYMERS,  300  (120260).  [PMID:36372488] [10.1016/j.carbpol.2022.120260]
9. Lingxia Qin, Xinxin Ren, Kaiyue Hu, Di Wu, Zhiyong Guo, Sui Wang, Linwen Jiang, Yufang Hu.  (2022)  Supramolecular Host-Guest Interaction-Driven Electrochemical Recognition for Pyrophosphate and Alkaline Phosphatase Analysis.  CHEMBIOCHEM,  23  (20): (e202200413).  [PMID:35997506] [10.1002/cbic.202200413]
10. Deng Hao-Hua, Yang Hui-Jing, Huang Kai-Yuan, Zheng Yi-Jing, Xu Ying-Ying, Peng Hua-Ping, Liu Yin-Huan, Chen Wei, Hong Guo-Lin.  (2022)  Antenna effect of pyridoxal phosphate on the fluorescence of mitoxantrone-silicon nanoparticles and its application in alkaline phosphatase assay.  ANALYTICAL AND BIOANALYTICAL CHEMISTRY,  414  (17): (4877-4884).  [PMID:35576012] [10.1007/s00216-022-04110-7]
11. Yuan Yuan, Yuhan He, Dongni Pei, Liujuan Tong, Shengqiang Hu, Lin Liu, Xinyao Yi, Jianxiu Wang.  (2022)  Urease-Functionalized Near-Infrared Light-Responsive Gold Nanoflowers for Rapid Detection of Urea by a Portable Pressure Meter.  MICROCHEMICAL JOURNAL,  179  (107450).  [PMID:] [10.1016/j.microc.2022.107450]
12. Fei Qu, Zhuo Wang, Cong Li, Dafeng Jiang, Xian-en Zhao.  (2022)  Peptide cleavage-mediated aggregation-enhanced emission from metal nanoclusters for detecting trypsin and screen its inhibitors from foods.  SENSORS AND ACTUATORS B-CHEMICAL,  359  (131610).  [PMID:] [10.1016/j.snb.2022.131610]
13. Huan Wang, Hui Zhang, Dagan Zhang, Jie Wang, Hui Tan, Tiantian Kong.  (2021)  Enzyme-functionalized structural color hydrogel particles for urea detection and elimination.  Journal of Cleaner Production,  315  (128149).  [PMID:] [10.1016/j.jclepro.2021.128149]
14. Qiaozhen Bao, Dan Lin, Yaoran Gao, Lina Wu, Jinhua Fu, Khuslen Galaa, Xinhua Lin, Liqing Lin.  (2021)  Ultrasensitive off-on-off fluorescent nanosensor for protamine and trypsin detection based on inner-filter effect between N,S-CDs and gold nanoparticles.  MICROCHEMICAL JOURNAL,  168  (106409).  [PMID:] [10.1016/j.microc.2021.106409]
15. Zhanlin Zhang, Dandan Zhang, Bo Qiu, Wenxiong Cao, Yuan Liu, Qingjie Liu, Xiaohong Li.  (2021)  Icebreaker-inspired Janus nanomotors to combat barriers in the delivery of chemotherapeutic agents.  Nanoscale,  13  (13): (6545-6557).  [PMID:33885534] [10.1039/D0NR08853F]
16. Zhanlin Zhang, Hui Yan, Shang Li, Yuan Liu, Pan Ran, Weijia Chen, Xiaohong Li.  (2021)  Janus rod-like micromotors to promote the tumor accumulation and cell internalization of therapeutic agents.  CHEMICAL ENGINEERING JOURNAL,  404  (127073).  [PMID:] [10.1016/j.cej.2020.127073]
17. Jue Zhang, Bingqing Yan, Chao He, Yuanyuan Hao, Shudong Sun, Weifeng Zhao, Changsheng Zhao.  (2020)  Urease-Immobilized Magnetic Graphene Oxide as a Safe and Effective Urea Removal Recyclable Nanocatalyst for Blood Purification.  INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH,  59  (19): (8955–8964).  [PMID:] [10.1021/acs.iecr.0c00302]
18. Jue Zhang, Zhenqiang Shi, Chao He, Xin Song, Ye Yang, Shudong Sun, Weifeng Zhao, Changsheng Zhao.  (2020)  Urease immobilized GO core@shell heparin-mimicking polymer beads with safe and effective urea removal for blood purification.  INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,  156  (1503).  [PMID:31783078] [10.1016/j.ijbiomac.2019.11.197]
19. Zhoujiang Chen, Tian Xia, Zhanlin Zhang, Songzhi Xie, Tao Wang, Xiaohong Li.  (2019)  Enzyme-powered Janus nanomotors launched from intratumoral depots to address drug delivery barriers.  CHEMICAL ENGINEERING JOURNAL,  375  (122109).  [PMID:] [10.1016/j.cej.2019.122109]
20. Yang Hu, Yan Sun.  (2019)  Autonomous motion of immobilized enzyme on Janus particles significantly facilitates enzymatic reactions.  BIOCHEMICAL ENGINEERING JOURNAL,  149  (107242).  [PMID:] [10.1016/j.bej.2019.107242]
21. Qu Fei, Ding Yanru, Lv Xiaoxia, Xia Lian, You Jinmao, Han Wenli.  (2019)  Emissions of terbium metal–organic frameworks modulated by dispersive/agglomerated gold nanoparticles for the construction of prostate-specific antigen biosensor.  ANALYTICAL AND BIOANALYTICAL CHEMISTRY,  411  (17): (3979-3988).  [PMID:31089787] [10.1007/s00216-019-01883-2]
22. Muhammad Bilal, Hafiz M.N. Iqbal.  (2019)  Naturally-derived biopolymers: Potential platforms for enzyme immobilization.  INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,  130  (462).  [PMID:30825566] [10.1016/j.ijbiomac.2019.02.152]
23. Jue Zhang, Zhoujun Wang, Chao He, Xiaoling Liu, Weifeng Zhao, Shudong Sun, Changsheng Zhao.  (2019)  Safe and Effective Removal of Urea by Urease-Immobilized, Carboxyl-Functionalized PES Beads with Good Reusability and Storage Stability.  ACS Omega,  (2): (2853–2862).  [PMID:] [10.1021/acsomega.8b03287]
24. Li Fu, Yong Huang, Wen Cai, Huaiwei Zhang, Jiehui Yang, Dihua Wu, Weitao Su.  (2018)  Improving the hydrophilicity and chronocoulometric performance of TiO2 nanotubular arrays by Sr@Si doping.  CERAMICS INTERNATIONAL,  44  (19926).  [PMID:] [10.1016/j.ceramint.2018.07.256]
25. Jing Ouyang, Dawei Mu, Yi Zhang, Huaming Yang, Steven L. Suib.  (2018)  Selective Fabrication of Barium Carbonate Nanoparticles in the Lumen of Halloysite Nanotubes.  Minerals,  (7): (296).  [PMID:] [10.3390/min8070296]
26. Wenyue Xie, Lingli Lei, Meiling Tian, Zeying Zhang, Yingshuai Liu.  (2018)  A high-resolution colorimetric immunoassay platform realized by coupling enzymatic multicolor generation with smartphone readout.  ANALYST,  143  (12): (2901-2907).  [PMID:29808208] [10.1039/C8AN00382C]
27. Lv Miaomiao, Ma Xiaofei, Anderson Debbie P., Chang Peter R..  (2018)  Immobilization of urease onto cellulose spheres for the selective removal of urea.  CELLULOSE,  25  (1): (233-243).  [PMID:] [10.1007/s10570-017-1592-3]
28. Dong-Sheng Zhao, Li-Long Jiang, Ya-Xi Fan, Ling-Li Wang, Zhuo-Qing Li, Wei Shi, Ping Li, Hui-Jun Li.  (2017)  Investigation of Dioscorea bulbifera Rhizome-Induced Hepatotoxicity in Rats by a Multisample Integrated Metabolomics Approach.  CHEMICAL RESEARCH IN TOXICOLOGY,  30  (10): (1865–1873).  [PMID:28899093] [10.1021/acs.chemrestox.7b00176]
29. Kai Huang, Tao Zhang, Bo Jiang, Wanmeng Mu, Ming Miao.  (2016)  A coupled system involving arginase and urease for l-ornithine production.  JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC,  133  (S303).  [PMID:] [10.1016/j.molcatb.2017.01.018]

References

1. Xinyu Shao, Lulu Cao, Lixia Lu.  (2024)  Ultrasensitive detection of glucose oxidase and alkaline phosphatase in milk based on valence regulated upconversion nanoprobes.  FOOD CHEMISTRY,  432  (137212).  [PMID:37634343] [10.1016/j.foodchem.2023.137212]
2. Zhao Chang, Xiao Yang, Chu Jian, Hu Ran, Liu Hanlong, He Xiang, Liu Yi, Jiang Xiang.  (2023)  Microfluidic experiments of biological CaCO3 precipitation in transverse mixing reactive environments.  Acta Geotechnica,  18  (10): (5299-5318).  [PMID:] [10.1007/s11440-023-01938-w]
3. Pengyu Xi, Xuecheng He, Chuan Fan, Qinglin Zhu, Zehua Li, Yuemeng Yang, Xin Du, Tailin Xu.  (2023)  Smart Janus fabrics for one-way sweat sampling and skin-friendly colorimetric detection.  TALANTA,  259  (124507).  [PMID:37058940] [10.1016/j.talanta.2023.124507]
4. Liangliang Cheng, Wanlu Zheng, Ya-Nan Zhang, Xuegang Li, Yong Zhao.  (2023)  Highly Sensitive Fiber-Optic SPR Urea Sensor Based on ZIF-8/Urease.  IEEE TRANSACTIONS ON INSTRUMENTATION AND MEASUREMENT,  72  (1-7).  [PMID:] [10.1109/TIM.2023.3259040]
5. Yuyang Bei, Kamile Arkin, Yuxin Zheng, Xuesong Ma, Jie Zhao, Huimin Jin, Qingkun Shang.  (2023)  Construction of a ratiometric fluorescent probe for visual detection of urea in human urine based on carbon dots prepared from Toona sinensis leaves and 5-carboxyfluorescein.  ANALYTICA CHIMICA ACTA,  1240  (340733).  [PMID:36641152] [10.1016/j.aca.2022.340733]
6. Wanying Cui, Chunmiao Xia, Sheng Xu, Xinke Ye, Yihao Wu, Shukai Cheng, Rongli Zhang, Cuige Zhang, Zongcheng Miao.  (2023)  Water-in-water emulsions stabilized by self-assembled chitosan colloidal particles.  CARBOHYDRATE POLYMERS,  303  (120466).  [PMID:36657862] [10.1016/j.carbpol.2022.120466]
7. Xuecui Mei, Jiao Yang, Jiang Liu, Yingchun Li.  (2023)  Wearable, nanofiber-based microfluidic systems with integrated electrochemical and colorimetric sensing arrays for multiplex sweat analysis.  CHEMICAL ENGINEERING JOURNAL,  454  (140248).  [PMID:] [10.1016/j.cej.2022.140248]
8. Ming Zhao, Zhenhai Chen, Li Hao, Huayao Chen, Xinhua Zhou, Hongjun Zhou.  (2023)  CMC based microcapsules for smart delivery of pesticides with reduced risks to the environment.  CARBOHYDRATE POLYMERS,  300  (120260).  [PMID:36372488] [10.1016/j.carbpol.2022.120260]
9. Lingxia Qin, Xinxin Ren, Kaiyue Hu, Di Wu, Zhiyong Guo, Sui Wang, Linwen Jiang, Yufang Hu.  (2022)  Supramolecular Host-Guest Interaction-Driven Electrochemical Recognition for Pyrophosphate and Alkaline Phosphatase Analysis.  CHEMBIOCHEM,  23  (20): (e202200413).  [PMID:35997506] [10.1002/cbic.202200413]
10. Deng Hao-Hua, Yang Hui-Jing, Huang Kai-Yuan, Zheng Yi-Jing, Xu Ying-Ying, Peng Hua-Ping, Liu Yin-Huan, Chen Wei, Hong Guo-Lin.  (2022)  Antenna effect of pyridoxal phosphate on the fluorescence of mitoxantrone-silicon nanoparticles and its application in alkaline phosphatase assay.  ANALYTICAL AND BIOANALYTICAL CHEMISTRY,  414  (17): (4877-4884).  [PMID:35576012] [10.1007/s00216-022-04110-7]
11. Yuan Yuan, Yuhan He, Dongni Pei, Liujuan Tong, Shengqiang Hu, Lin Liu, Xinyao Yi, Jianxiu Wang.  (2022)  Urease-Functionalized Near-Infrared Light-Responsive Gold Nanoflowers for Rapid Detection of Urea by a Portable Pressure Meter.  MICROCHEMICAL JOURNAL,  179  (107450).  [PMID:] [10.1016/j.microc.2022.107450]
12. Fei Qu, Zhuo Wang, Cong Li, Dafeng Jiang, Xian-en Zhao.  (2022)  Peptide cleavage-mediated aggregation-enhanced emission from metal nanoclusters for detecting trypsin and screen its inhibitors from foods.  SENSORS AND ACTUATORS B-CHEMICAL,  359  (131610).  [PMID:] [10.1016/j.snb.2022.131610]
13. Huan Wang, Hui Zhang, Dagan Zhang, Jie Wang, Hui Tan, Tiantian Kong.  (2021)  Enzyme-functionalized structural color hydrogel particles for urea detection and elimination.  Journal of Cleaner Production,  315  (128149).  [PMID:] [10.1016/j.jclepro.2021.128149]
14. Qiaozhen Bao, Dan Lin, Yaoran Gao, Lina Wu, Jinhua Fu, Khuslen Galaa, Xinhua Lin, Liqing Lin.  (2021)  Ultrasensitive off-on-off fluorescent nanosensor for protamine and trypsin detection based on inner-filter effect between N,S-CDs and gold nanoparticles.  MICROCHEMICAL JOURNAL,  168  (106409).  [PMID:] [10.1016/j.microc.2021.106409]
15. Zhanlin Zhang, Dandan Zhang, Bo Qiu, Wenxiong Cao, Yuan Liu, Qingjie Liu, Xiaohong Li.  (2021)  Icebreaker-inspired Janus nanomotors to combat barriers in the delivery of chemotherapeutic agents.  Nanoscale,  13  (13): (6545-6557).  [PMID:33885534] [10.1039/D0NR08853F]
16. Zhanlin Zhang, Hui Yan, Shang Li, Yuan Liu, Pan Ran, Weijia Chen, Xiaohong Li.  (2021)  Janus rod-like micromotors to promote the tumor accumulation and cell internalization of therapeutic agents.  CHEMICAL ENGINEERING JOURNAL,  404  (127073).  [PMID:] [10.1016/j.cej.2020.127073]
17. Jue Zhang, Bingqing Yan, Chao He, Yuanyuan Hao, Shudong Sun, Weifeng Zhao, Changsheng Zhao.  (2020)  Urease-Immobilized Magnetic Graphene Oxide as a Safe and Effective Urea Removal Recyclable Nanocatalyst for Blood Purification.  INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH,  59  (19): (8955–8964).  [PMID:] [10.1021/acs.iecr.0c00302]
18. Jue Zhang, Zhenqiang Shi, Chao He, Xin Song, Ye Yang, Shudong Sun, Weifeng Zhao, Changsheng Zhao.  (2020)  Urease immobilized GO core@shell heparin-mimicking polymer beads with safe and effective urea removal for blood purification.  INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,  156  (1503).  [PMID:31783078] [10.1016/j.ijbiomac.2019.11.197]
19. Zhoujiang Chen, Tian Xia, Zhanlin Zhang, Songzhi Xie, Tao Wang, Xiaohong Li.  (2019)  Enzyme-powered Janus nanomotors launched from intratumoral depots to address drug delivery barriers.  CHEMICAL ENGINEERING JOURNAL,  375  (122109).  [PMID:] [10.1016/j.cej.2019.122109]
20. Yang Hu, Yan Sun.  (2019)  Autonomous motion of immobilized enzyme on Janus particles significantly facilitates enzymatic reactions.  BIOCHEMICAL ENGINEERING JOURNAL,  149  (107242).  [PMID:] [10.1016/j.bej.2019.107242]
21. Qu Fei, Ding Yanru, Lv Xiaoxia, Xia Lian, You Jinmao, Han Wenli.  (2019)  Emissions of terbium metal–organic frameworks modulated by dispersive/agglomerated gold nanoparticles for the construction of prostate-specific antigen biosensor.  ANALYTICAL AND BIOANALYTICAL CHEMISTRY,  411  (17): (3979-3988).  [PMID:31089787] [10.1007/s00216-019-01883-2]
22. Muhammad Bilal, Hafiz M.N. Iqbal.  (2019)  Naturally-derived biopolymers: Potential platforms for enzyme immobilization.  INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,  130  (462).  [PMID:30825566] [10.1016/j.ijbiomac.2019.02.152]
23. Jue Zhang, Zhoujun Wang, Chao He, Xiaoling Liu, Weifeng Zhao, Shudong Sun, Changsheng Zhao.  (2019)  Safe and Effective Removal of Urea by Urease-Immobilized, Carboxyl-Functionalized PES Beads with Good Reusability and Storage Stability.  ACS Omega,  (2): (2853–2862).  [PMID:] [10.1021/acsomega.8b03287]
24. Li Fu, Yong Huang, Wen Cai, Huaiwei Zhang, Jiehui Yang, Dihua Wu, Weitao Su.  (2018)  Improving the hydrophilicity and chronocoulometric performance of TiO2 nanotubular arrays by Sr@Si doping.  CERAMICS INTERNATIONAL,  44  (19926).  [PMID:] [10.1016/j.ceramint.2018.07.256]
25. Jing Ouyang, Dawei Mu, Yi Zhang, Huaming Yang, Steven L. Suib.  (2018)  Selective Fabrication of Barium Carbonate Nanoparticles in the Lumen of Halloysite Nanotubes.  Minerals,  (7): (296).  [PMID:] [10.3390/min8070296]
26. Wenyue Xie, Lingli Lei, Meiling Tian, Zeying Zhang, Yingshuai Liu.  (2018)  A high-resolution colorimetric immunoassay platform realized by coupling enzymatic multicolor generation with smartphone readout.  ANALYST,  143  (12): (2901-2907).  [PMID:29808208] [10.1039/C8AN00382C]
27. Lv Miaomiao, Ma Xiaofei, Anderson Debbie P., Chang Peter R..  (2018)  Immobilization of urease onto cellulose spheres for the selective removal of urea.  CELLULOSE,  25  (1): (233-243).  [PMID:] [10.1007/s10570-017-1592-3]
28. Dong-Sheng Zhao, Li-Long Jiang, Ya-Xi Fan, Ling-Li Wang, Zhuo-Qing Li, Wei Shi, Ping Li, Hui-Jun Li.  (2017)  Investigation of Dioscorea bulbifera Rhizome-Induced Hepatotoxicity in Rats by a Multisample Integrated Metabolomics Approach.  CHEMICAL RESEARCH IN TOXICOLOGY,  30  (10): (1865–1873).  [PMID:28899093] [10.1021/acs.chemrestox.7b00176]
29. Kai Huang, Tao Zhang, Bo Jiang, Wanmeng Mu, Ming Miao.  (2016)  A coupled system involving arginase and urease for l-ornithine production.  JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC,  133  (S303).  [PMID:] [10.1016/j.molcatb.2017.01.018]

Solution Calculators