mAb (K05002_3F6); Mouse anti V5 tag Antibody; WB, IP; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab133823-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $59.90 | |
| Ab133823-50μl | 50μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $169.90 | |
| Ab133823-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $249.90 | |
| Ab133823-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $1,899.90 |
mAb (K05002_3F6); Mouse anti V5 tag Antibody; WB, IP; Unconjugated
| Product Name | V5 tag Mouse mAb |
|---|---|
| Specifications & Purity | Carrier Free, ExactAb™, Validated, High performance, 1.0 mg/mL |
| Host species | Mouse |
| Specificity | V5 |
| Immunogen | A fusion protein containing V5. |
| Positive Control | WB: HEK293 cells transfected with T7 tag. IP: HEK-293 cell lysates transfected with V5 tagged CDA at C-terminus; HEK-293 cell lysates transfected with V5 tagged CDA at C-terminus and KT82 was used as IgG isotype control antibody; HEK-293 cell lysates tran |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, High performance, Validated |
| Product Description |
Mouse anti V5 tag Antibody, Monoclonal (K05002_3F6), could be used for WB, IP and so on. |
| Isotype | Mouse IgG2a |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -80°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 2-8 ℃ (3 months). Store at -20 ℃ (12 months). Store at -80 ℃ (36 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
V5 tag Mouse mAb (Ab133823) - Western Blot
All lanes: V5 tag Mouse mAb (Ab133823) at 1/4000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 115 kDa
Observed band size: 50, 112 kDa
Exposure time: 1.16 s
V5 tag Mouse mAb (Ab133823) - IP
Immunoprecipitation was performed by incubation of 2.5 µg of V5 tag Mouse mAb (Ab133823) with 200 µg of lysate extracted from HEK293 transfected with V5 tagged CDA at C-terminus. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. V5 tag antibody at 1.0 µg/mL was used as the V5 tag Mouse mAb (Ab133823) and peroxidase conjugated goat anti-mouse IgG (Fc specific) was used as the secondary antibody. Lane 1: HEK293 lysate (transfected with V5 tagged CDA at C-terminus).Lane 2: V5 tagged protein immunoprecipitated from the same lysate as Lane1. Lane 3: IgG isotype control antibody instead of V5 tag Mouse mAb (Ab133823) in HEK293 lysate.Result: V5 tag Mouse mAb (Ab133823) can immunoprecipitate V5 tag.
V5 tag Mouse mAb (Ab133823) - IP
Immunoprecipitation was performed by incubation of 2.5 µg of V5 tag Mouse mAb (Ab133823) with 200 µg of lysate extracted from HEK293 transfected with V5 tagged ARF3 at N-terminus. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. V5 tag protein at 1.0 µg/mL was used as the V5 tag Mouse mAb (Ab133823) and peroxidase conjugated goat anti-mouse IgG (Fc specific) was used as the secondary antibody.Lane 1: HEK-293 lysate (transfected with V5 tagged ARF3 at N-terminus).Lane 2: V5 tagged protein immunoprecipitated from the same lysate as Lane 1.Lane 3: IgG isotype control antibody instead of V5 tag Mouse mAb (Ab133823) in HEK293 lysate.Result: V5 tag Mouse mAb (Ab133823) can immunoprecipitate V5 tag.
V5 tag Mouse mAb (Ab133823) - IP
Immunoprecipitation was performed by incubation of 2.5 µg of V5 tag Mouse mAb (Ab133823) with untransfected HEK293 lysate containing 200 µg of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. V5 tag Mouse mAb (Ab133823) at 1.0 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG (Fc specific) was used as the secondary antibody.Lane 1: Untransfected HEK293 lysate.Lane 2: Sample immunoprecipitated from untransfected HEK-293 lysate by KT25.Lane 3: IgG isotype control antibody instead of V5 tag Mouse mAb (Ab133823) in HEK293 lysate.Result: Unspecific immunoprecipitation by V5 tag Mouse mAb (Ab133823) was not obsearved.
| Application | Dilution info |
|---|---|
| IP | 1/100 |
| WB | 1/3000-1/4000 |
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0708830 | Certificate of Analysis | Jul 26, 2024 | Ab133823 |
| ZJ24F0708829 | Certificate of Analysis | Jul 26, 2024 | Ab133823 |
| ZJ24F0708828 | Certificate of Analysis | Jul 26, 2024 | Ab133823 |
