Yeast extract，powder - Yeast extract dry powder, high purity , CAS No.8013-01-2

Item Number

Y278674

• Synonyms: 8013-01-2 | 2-(11H-benzo[a]fluoren-11-yl)acetic acid | 11H-benzo[a]fluoren-11-ylacetic acid | 2-{11H-benzo[a]fluoren-11-yl}acetic acid | CHEBI:174614 | AKOS015906550 | 2-(11H-benzo[a]luoren-11-yl)acetic acid | 2-(11H-benzo[a]fluoren-11-yl)-acetic acid
• Specifications & Purity: Yeast extract dry powder
• Storage Temp: Protected from light,Desiccated
• Shipped In: Normal

Product Description

Product Introduction

Purified and cultured primary yeast is used as raw material, and special extraction and preparation processes are adopted to maximize the enrichment and retention of nutritional factors in the primary yeast. It is a medium supplement without animal sources.

Description

Light yellow powder, soluble in water, easy to absorb moisture;

Components

Rich in free amino acids (especially glutamic acid and alanine), peptides (especially rich in small peptides), B vitamins, nucleic acids, nucleotides, and other nutritional factors

Application

As a high-efficiency organic nutrient source for the cultivation of difficult-to-cultivation and auxotrophic or severe microorganisms, for example, for the rapid identification and cultivation of mycoplasma, influenza and Haemophilus parainfluenzae, preparation of diphtheria, tetanus antigen culture medium, etc. It is used as a nutrient supplement or supplement for serum-free cell culture medium in cell culture applications.

Directions

After opening the product, it can be directly added in proportion or formulated into a certain concentration solution, and added in proportion to use after filtration and sterilization. The unused products shall be sealed and preserved immediately. Recommended dosage: 0.5%-1.5% (w/v) for animal mycoplasma culture; 1%-2.5% (w/v) for avian mycoplasma culture.

Physical-chemical characteristics

Test items	index
Solubility (%, normal temperature)	30%, clear and translucent
pH (2% solution)	5.5-7.0
Moisture (%)	4-6
Total nitrogen (on dry basis, %)	＞9.0
Amino nitrogen (on a dry basis, %)	＞4.5
Ash content (%) NaCl（%）	≤15 ≤2
Endotoxin (EU/g)	≤200

Microbiology

Test items	index
Total number of colonies (cfu/g)	≤10000
Coliform group (MPN/g)  Yeast (cfu/g) Mold (cfu/g) Salmonella/25g Shigella/25g	≤0.3 ≤10.0 ≤10.0 Not check out Not check out
Staphylococcus aureus, /25g	Not check out

Average vitamin nucleotide composition

Test items	index
B1（ppm） B2（ppm） Biotin (ug/100g) CMP （%） UMP （%） GMP （%）	3.8 80 630 0.17 0.15 0.05
AMP （%）	0.22

ps :The above indicators are for reference only, the specific values are subject to the actual measurement of each batch

molecular weight distribution

＞2000	2000-1000	1000-400	400-180	＜180
0	0.68	12.18	64.232	22.91

Amino acid composition

	free	hydrolysis
ASP Thr	0.47 0.97	3.48 1.38
Ser	0.75	1.37
Glu	7.56	15.60
Gly	0.27	1.51
Ala	9.76	10.00
Cys-cys	0.76	0.99
Val	1.65	2.32
Met	0.13	0.36
Ile	0.50	1.02

Leu	0.75	1.45
Tyr	0.41	0.77
Phe	0.70	1.25
Lys	0.55	1.78
NH3	0.64	1.55
His	0.22	0.54
Arg	1.29	1.78
Pro	0.38	0.77
sum	27.76	47.92