Product Introduction
Purified and cultured primary yeast is used as raw material, and special extraction and preparation processes are adopted to maximize the enrichment and retention of nutritional factors in the primary yeast. It is a medium supplement without animal sources.
Description
Light yellow powder, soluble in water, easy to absorb moisture;
Components
Rich in free amino acids (especially glutamic acid and alanine), peptides (especially rich in small peptides), B vitamins, nucleic acids, nucleotides, and other nutritional factors
Application
As a high-efficiency organic nutrient source for the cultivation of difficult-to-cultivation and auxotrophic or severe microorganisms, for example, for the rapid identification and cultivation of mycoplasma, influenza and Haemophilus parainfluenzae, preparation of diphtheria, tetanus antigen culture medium, etc. It is used as a nutrient supplement or supplement for serum-free cell culture medium in cell culture applications.
Directions
After opening the product, it can be directly added in proportion or formulated into a certain concentration solution, and added in proportion to use after filtration and sterilization. The unused products shall be sealed and preserved immediately. Recommended dosage: 0.5%-1.5% (w/v) for animal mycoplasma culture; 1%-2.5% (w/v) for avian mycoplasma culture.
Physical-chemical characteristics
Test items
| index
| Solubility (%, normal temperature)
| 30%, clear and translucent
| pH (2% solution)
| 5.5-7.0 | Moisture (%) | 4-6 | Total nitrogen (on dry basis, %)
| ＞9.0 | Amino nitrogen (on a dry basis, %)
| ＞4.5 | Ash content (%)
NaCl（%）
| ≤15 ≤2
| Endotoxin (EU/g)
| ≤200
|
Microbiology
Test items
| index
| Total number of colonies (cfu/g)
| ≤10000 | Coliform group (MPN/g) Yeast (cfu/g)
Mold (cfu/g)
Salmonella/25g
Shigella/25g
| ≤0.3
≤10.0
≤10.0
Not check out
Not check out
| Staphylococcus aureus, /25g
| Not check out
|
Average vitamin nucleotide composition
Test items
| index
| B1（ppm）
B2（ppm）
Biotin (ug/100g)
CMP （%）
UMP （%）
GMP （%）
| 3.8
80 630
0.17
0.15
0.05
| AMP （%） | 0.22
|
ps :The above indicators are for reference only, the specific values are subject to the actual measurement of each batch
molecular weight distribution
＞2000 | 2000-1000 | 1000-400 | 400-180 | ＜180 | 0 | 0.68 | 12.18 | 64.232 | 22.91 |
Amino acid composition
| free | hydrolysis
| ASP Thr
| 0.47
0.97 | 3.48 1.38
|
|
|
| Ser | 0.75 | 1.37 | Glu | 7.56 | 15.60 | Gly | 0.27 | 1.51 | Ala | 9.76 | 10.00 | Cys-cys | 0.76 | 0.99 | Val | 1.65 | 2.32 | Met | 0.13 | 0.36 | Ile | 0.50 | 1.02 |
Leu | 0.75 | 1.45 | Tyr | 0.41 | 0.77 | Phe | 0.70 | 1.25 | Lys | 0.55 | 1.78 | NH3 | 0.64 | 1.55 | His | 0.22 | 0.54 | Arg | 1.29 | 1.78 | Pro | 0.38 | 0.77 | sum | 27.76 | 47.92 |
| 
