Yeast extract，powder - For cell culture, high purity , CAS No.8013-01-2

Item Number

Y278676

• Synonyms: 8013-01-2|2-(11H-benzo[a]fluoren-11-yl)acetic acid|11H-benzo[a]fluoren-11-ylacetic acid|2-{11H-benzo[a]fluoren-11-yl}acetic acid|CHEBI:174614|AKOS015906550|2-(11H-benzo[a]luoren-11-yl)acetic acid|2-(11H-benzo[a]fluoren-11-yl)-acetic acid
• Specifications & Purity: For cell culture
• Storage Temp: Desiccated
• Shipped In: Normal
• Grade: for cell culture

Product Description

Product Introduction

The baker's yeast cultivated by high-density fermentation control technology is used as the raw material, and the powdery product obtained by concentration and spray drying using bio-oriented degradation and membrane filtration technology. This product is rich in nutritional components such as peptides, B vitamins, nucleotides, and has nutritional factors required to maintain cell growth and proliferation and promote cell protein expression. As a non-animal source medium raw material, it can realize animal serum in the medium Substitution of components. The endotoxin content of this product is less than 100EU/g.

Description

The product is a light yellow powder with good fluidity and uniform particles, which is easy to absorb moisture and is easily soluble in water.

√ The batch uniformity can be guaranteed;

√ Dry powder is easy to store and has good solubility, and it is also suitable for storage of high-concentration mother liquor;

√ Non-animal origin, no pathogenic factors, no GMO, no allergenic ingredients, low endotoxin content;

√ The components are clear and quantifiable, and the degree of protein hydrolysis is high;

√ Promote growth, stabilize proliferation, lower serum, and increase yield;

Application

As a supplement to protein, peptide, amino acid, carbohydrate, and micronutrient factors in the culture medium, it is suitable for vaccines, recombinant protein expression, insulin, interferon, monoclonal antibodies, and mammalian cells, insect cell culture.

Physical-chemical characteristics(Typical value)

SKU	Source	Hydrolysis  method	Endotoxin	pH value	Solubility
Y278676	yeast	Enzymatic hydrolysis	<100EU/g	7±0.2	40%

SKU	AN(%)	TN(%)	AN/TN(%)	Ash content (%)	Moisture (%)
Y278676	5.56	12.09	45.99	10.30	4.08

SKU	molecular weight distribution
	≥2kDa	2-1 kDa	1k-400 Da	400-180 Da	<180 Da
Y278676	0.6	1.14	20.06	56.55	21.65

SKU	Minerals （ mg/kg）
	calcium	iron	magnesium	Potassium	sodium
Y278676	421.03	43	2149.98	37599.5	22098.87

The total amino acid content of the product (g/100g) (typical value)

SKU	Ala	Arg	Asp	Cys-cys	Glu	Lys	His	Ile	Met
Y278676	5.71	2.11	1.66	4.93	4.93	1.86	0.51	1.73	0.67

SKU	Phe	Pro	Ser	Thr	Tyr	Val	Free sum	Total amino acid
Y278676	1.36	0.66	1.48	1.91	0.88	2.31	32.05	60.53

References

Sung YH, Lim SW, Chung JY, Lee GM (2004) Yeast hydrolysate as a low-cost additive to serum-free medium for the production of human thrombopoietin in suspension cultures of Chinese hamster ovary cells. Appl Microbiol Biotechnol 63(5):527–536

Hu DD, Sun YT, Liu XP, Liu JT(2015) Understanding the intracellular effects of yeast extract on the enhancement of Fc-fusion protein production in Chinese hamster ovary cell culture. Appl Microbiol Biotechnol 99:8429–8440

Spearman M, Chan S, Jung V, Kowbel V(2016) Components of yeast (Sacchromyces cervisiae) extract as defined media additives that support the

growth and productivity of CHO cells. Journal of Biotechnology 233:129-142