1,4-Dithiothreitol (DTT) - ≥97%, high purity , CAS No.3483-12-3

Item Number

D265376

• Synonyms: Dithiothreitol|DL-1,4-Dithiothreitol|dl-Dithiothreitol|3483-12-3|(2S,3S)-1,4-DIMERCAPTOBUTANE-2,3-DIOL|1,4-Dithiothreitol|D-1,4-Dithiothreitol|D-Dithiothreitol|27565-41-9|D-Dtt|1,4-Dithio-dl-threitol|threo-1,4-Dimercapto-2,3-butanediol|(2S,3S)-1,4-disulfa
• Specifications & Purity: ≥97%
• Storage Temp: Store at 2-8°C,Protected from light,Argon charged
• Shipped In: Wet ice

Product Description

Overview

Reducing agent

Application

Use 1,4-Dithiothreitol primarily to protect free SH-groups from oxidation. Use it routinely in all work with enzymes and proteins during enzyme measurement and the characterization of proteins.

• As one of the reduction and alkylation reactants of the main allergen αs1-casein in milk.

• As a component of sperm membrane removal and reactivation medium.

•Because mercaptans can effectively protect the active sites of biocatalysts and maintain the stability of enzymes.

• As a reducing agent to test the substitution of N-alkaline maleic substitution and the reaction of excess radicals.

Before SDS-PAGE, DTT can effectively reduce protein disulfide bonds in the sample buffer. Both the irritation and toxicity of DTT are reduced by 2-mercaptoethanol. Usually, the required concentration of DTT (100mM) is used. It uses one-seventh of the concentration of 2-cholesterol-based ethanol (5% v/v, 700 mM).

Benefits

1.Rely on the higher stability of DTT in comparison to 2-mercaptoethanol

in aqueous solution

2.Experience more convenience and a less disagreeable odor

3.Benefit from the reduced tendency to oxidize in air.

Specification

Appearance: White to yellowish crystallizate

DTT (with Ellman's reagent): ≥97%

Thin layer chromatography: Chromatographically homogeneous, corresponds to reference

Stability: At +2 to +8°C within specification range for 24 months. Store dry. Protect from light.