| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab086628-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $56.90 | |
| Ab086628-50μl | 50μl | In stock | $169.90 | |
| Ab086628-100μl | 100μl | In stock | $219.90 | |
| Ab086628-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $1,999.90 |
mAb(C5); Mouse anti Human 14-3-3 zeta Antibody; WB; Unconjugated
| Product Name | 14-3-3 zeta Mouse mAb - Primary antibody, specific to YWHAZ, Mouse IgG2b |
|---|---|
| Synonyms | 1433 zeta | 14-3-3 zeta | KCIP-1 | KCIP-1MGC126532,14-3-3-zeta | MGC111427 | MGC138156 | tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, deltapolypeptide | tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zet |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Azide Free, High performance, Lot by Lot |
| Host species | Mouse |
| Specificity | YWHAZ |
| Immunogen | Recombinant YWHAz expressed in E.coli (AA 1-245) |
| Positive Control | WB: Raji, Jurkat, HeLa, A431, MCF7, A549, NIH/3T3 and C6 cell lysates. |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, High Performance, Validated |
| Note | 20μl卖完停产，不再备货 |
| Product Description | Mouse anti Human 14-3-3 zeta Antibody, Monoclonal(C5), could be used for WB and so on . Application: WB: 0.01-2 µg/mL Protein Function Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. |
| Isotype | Mouse IgG2b |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A + Protein G purified |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
14-3-3 zeta Mouse mAb (Ab086628) - Western Blot
All lanes: 14-3-3 zeta Mouse mAb (Ab086628) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa
Exposure time: 2.5 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
| Application | Dilution info |
|---|---|
| WB |
0.01-2 µg/mL
|
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F1101813 | Certificate of Analysis | Nov 27, 2023 | Ab086628 |
| ZJ23F1101812 | Certificate of Analysis | Nov 27, 2023 | Ab086628 |
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| 2. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330] |
| 3. Dennis M Krüger,Adrian Glas,David Bier,Nicole Pospiech,Kerstin Wallraven,Laura Dietrich,Christian Ottmann,Oliver Koch,Sven Hennig,Tom N Grossmann. (2017-10-14) Structure-Based Design of Non-natural Macrocyclic Peptides That Inhibit Protein-Protein Interactions.. Journal of medicinal chemistry, 60 ((21)): (8982-8988). [PMID:29028171] |
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| 6. Dubois, T T and 7 more authors.. (1997) 14-3-3 is phosphorylated by casein kinase I on residue 233. Phosphorylation at this site in vivo regulates Raf/14-3-3 interaction.. The Journal of biological chemistry, (14): [PMID:9360956] |
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| 9. Rittinger, K K and 7 more authors.. (1999) Structural analysis of 14-3-3 phosphopeptide complexes identifies a dual role for the nuclear export signal of 14-3-3 in ligand binding.. Molecular cell, [PMID:10488331] |
| 10. Obsil, T T, Ghirlando, R R, Klein, D C DC, Ganguly, S S and Dyda, F F.. (2001) Crystal structure of the 14-3-3zeta:serotonin N-acetyltransferase complex. a role for scaffolding in enzyme regulation.. Cell, (20): [PMID:11336675] |
| 11. Ganguly, S S and 13 more authors.. (2001) Role of a pineal cAMP-operated arylalkylamine N-acetyltransferase/14-3-3-binding switch in melatonin synthesis.. Proceedings of the National Academy of Sciences of the United States of America, (3): [PMID:11427721] |
| 12. Powell, David W DW, Rane, Madhavi J MJ, Chen, Qingdan Q, Singh, Saurabh S and McLeish, Kenneth R KR.. (2002) Identification of 14-3-3zeta as a protein kinase B/Akt substrate.. The Journal of biological chemistry, (14): [PMID:11956222] |
| 13. Zhu, Peng cheng Pc and 8 more authors.. (2003) The interaction between ADAM 22 and 14-3-3zeta: regulation of cell adhesion and spreading.. Biochemical and biophysical research communications, (21): [PMID:12589811] |
| 14. Gevaert, Kris K and 6 more authors.. (2003) Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides.. Nature biotechnology, [PMID:12665801] |
| 15. Andersen, Jens S JS and 5 more authors.. (2003) Proteomic characterization of the human centrosome by protein correlation profiling.. Nature, (4): [PMID:14654843] |
| 16. Zenke, Frank T FT and 5 more authors.. (2004) p21-activated kinase 1 phosphorylates and regulates 14-3-3 binding to GEF-H1, a microtubule-localized Rho exchange factor.. The Journal of biological chemistry, (30): [PMID:14970201] |
| 17. Nagata-Ohashi, Kyoko K and 11 more authors.. (2004) A pathway of neuregulin-induced activation of cofilin-phosphatase Slingshot and cofilin in lamellipodia.. The Journal of cell biology, (24): [PMID:15159416] |
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| 20. Obsilova, Veronika V and 7 more authors.. (2005) 14-3-3 Protein interacts with nuclear localization sequence of forkhead transcription factor FoxO4.. Biochemistry, (30): [PMID:16114898] |
| 21. Macdonald, Neil N and 12 more authors.. (2005) Molecular basis for the recognition of phosphorylated and phosphoacetylated histone h3 by 14-3-3.. Molecular cell, (28): [PMID:16246723] |
| 22. Gu, Young-Mi YM and 5 more authors.. (2006) Protein kinase A phosphorylates and regulates dimerization of 14-3-3 epsilon.. FEBS letters, (9): [PMID:16376338] |
| 23. Angrand, Pierre-Olivier PO and 12 more authors.. (2006) Transgenic mouse proteomics identifies new 14-3-3-associated proteins involved in cytoskeletal rearrangements and cell signaling.. Molecular & cellular proteomics : MCP, [PMID:16959763] |
| 24. Chi, An A and 16 more authors.. (2006) Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes.. Journal of proteome research, [PMID:17081065] |
| 25. Olsen, Jesper V JV and 6 more authors.. (2006) Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.. Cell, (3): [PMID:17081983] |
| 26. Ottmann, Christian C and 9 more authors.. (2007) Phosphorylation-independent interaction between 14-3-3 and exoenzyme S: from structure to pathogenesis.. The EMBO journal, (7): [PMID:17235285] |
| 27. Kim, Jun-Sub JS, Diebold, Becky A BA, Babior, Bernard M BM, Knaus, Ulla G UG and Bokoch, Gary M GM.. (2007) Regulation of Nox1 activity via protein kinase A-mediated phosphorylation of NoxA1 and 14-3-3 binding.. The Journal of biological chemistry, (30): [PMID:17913709] |
| 28. Daub, Henrik H and 9 more authors.. (2008) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle.. Molecular cell, (8): [PMID:18691976] |
| 29. Brummer, Tilman T and 11 more authors.. (2008) Phosphorylation-dependent binding of 14-3-3 terminates signalling by the Gab2 docking protein.. The EMBO journal, (3): [PMID:19172738] |
| 30. Kajiwara, Yuji Y, Buxbaum, Joseph D JD and Grice, Dorothy E DE.. (2009) SLITRK1 binds 14-3-3 and regulates neurite outgrowth in a phosphorylation-dependent manner.. Biological psychiatry, (15): [PMID:19640509] |
| 31. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332] |
| 32. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692] |
| 33. Mi, Lixin and 8 more authors.. (2011) Identification of potential protein targets of isothiocyanates by proteomics.. Chemical research in toxicology, (17): [PMID:21838287] |
| 34. Bienvenut, Willy V WV and 6 more authors.. (2012) Comparative large scale characterization of plant versus mammal proteins reveals similar and idiosyncratic N-α-acetylation features.. Molecular & cellular proteomics : MCP, [PMID:22223895] |
| 35. Van Damme, Petra P and 15 more authors.. (2012) N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.. Proceedings of the National Academy of Sciences of the United States of America, (31): [PMID:22814378] |
| 36. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
| 37. Greninger, Alexander L AL, Knudsen, Giselle M GM, Betegon, Miguel M, Burlingame, Alma L AL and DeRisi, Joseph L JL.. (2013) ACBD3 interaction with TBC1 domain 22 protein is differentially affected by enteroviral and kobuviral 3A protein binding.. mBio, (9): [PMID:23572552] |
| 38. Bian, Yangyang Y and 9 more authors.. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.. Journal of proteomics, (16): [PMID:24275569] |
| 39. Yuasa, Keizo K and 5 more authors.. (2015) Suppression of death-associated protein kinase 2 by interaction with 14-3-3 proteins.. Biochemical and biophysical research communications, (14): [PMID:26047703] |
| 40. Masters, Seth L SL and 31 more authors.. (2016) Familial autoinflammation with neutrophilic dermatosis reveals a regulatory mechanism of pyrin activation.. Science translational medicine, (30): [PMID:27030597] |
| 41. Popov, Ivan K IK and 9 more authors.. (2019) A YWHAZ Variant Associated With Cardiofaciocutaneous Syndrome Activates the RAF-ERK Pathway.. Frontiers in physiology, [PMID:31024343] |
| 42. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460] |
